The molecular mechanism of Suguhiratake-acute encephalopathy;

• Project/Area Number: 24248021
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Shizuoka University
• Principal Investigator: KAWAGISHI Hirokazu 静岡大学, グリーン科学技術研究所, 教授 (70183283)
• Co-Investigator(Kenkyū-buntansha): KAN Toshiyuki 静岡県立大学, 薬学部, 教授 (10221904) ; NAGAI Kaoru 山梨大学, 総合研究部, 准教授 (20340953) ; HIRAI Hirofumi 静岡大学, 農学研究科, 教授 (70322138) ; MORITA Tatsuya 静岡大学, 農学研究科, 教授 (90332692)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000); Fiscal Year 2014: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000); Fiscal Year 2013: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000); Fiscal Year 2012: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
• Keywords: キノコ / スギヒラタケ / 急性脳症 / pleurocybellaziridine / 分痔機構

Outline of Final Research Achievements

We have a hypothesis of the molecular mechanism of Suguhiratake-acute encephalopathy; the complex between two macromolecules, a lectin (PPL) and B3, destroyed blood brain barrier (BBB), then a low-molecular-weight toxin, pleurocybellaziridine (PA) attacked the brain and caused the unique lesion. To confirm the hypothesis, we obtained the following results.
1) PA was stable when it coexisted with PPL and B3. 2) PA showed toxicity against oligodendrocyte. 3) Genome database of Suguhiratake was established. 4) Expression systems of recombinant PPL and B3 were successfully constructed. 5) The complex of rB3 and rPPL exhibited proteinase activity and destroyed BBB.

Research Products

• [Journal Article] A-WINGS: an integrated genome database for Pleurocybella porrigens (Angel’s wing oyster mushroom, Sugihiratake) (2014)
  • Author(s): Naoki Yamamoto, Tomohiro Suzuki, Masaaki Kobayashi, Hideo Dohra, Yohei Sasaki, Hirofumi Hirai, Koji Yokoyama, Hirokazu Kawagishi and Kentaro Yano
  • Journal Title: BMC Research Notes Volume: 7 Issue: 1 Pages: 866-866
  • DOI: 10.1186/1756-0500-7-866
  • NAID: 120005575802
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Heterologous expression of a lectin from Pleurocybella porrigens (PPL) in Phanerochaete sordida YK-624 (2014)
  • Author(s): T. Suzuki, H. Dohra, S. Omae, Y. Takeshima, J-H. Choi, H. Hirai, H. Kawagishi
  • Journal Title: J. Microbiol. Meth. Volume: 100 Pages: 70-76
  • DOI: 10.1016/j.mimet.2014.02.016
  • NAID: 120005401892
  • Peer Reviewed
• [Journal Article] A new omics data resource of Pleurocybella porrigens for gene discovery (2013)
  • Author(s): T. Suzuki, K. Igarashi, H. Dohra, T. Someya, T. Takano, K. Harada, S. Omae, S., H. Hirai, K. Yano, H. Kawagishi
  • Journal Title: PloS ONE Volume: 8 Issue: 7 Pages: e69681-e69681
  • DOI: 10.1371/journal.pone.0069681
  • NAID: 120005304932
  • Peer Reviewed
• [Presentation] スギヒラタケ由来毒性高分子群異種発現系の構築 (2015)
  • Author(s): 亀澤哲弥、竹島愛乃、鈴木智大、河岸洋和、平井浩文
  • Organizer: 第65回日本木材学会大会
  • Place of Presentation: タワーホール船堀・東京都
  • Year and Date: 2015-03-18
• [Presentation] スギヒラタケ毒による急性脳症の分子機構とレクチンの関与 (2014)
  • Author(s): 河岸洋和
  • Organizer: 第33回日本糖質学会年会ワークショップ「真価を発揮し始めたGlyco-decoder分子群『レクチン』：構造生物学と進化工学の進展と医薬応用」
  • Place of Presentation: 名古屋大学・愛知県
  • Year and Date: 2014-08-11
  • Invited
• [Presentation] The angel’s wing mystery-Attempt to disclose the molecular mechanism of acute encephalopathy caused by eating angel’s wing oyster mushroom (Sugihiratake) (2012)
  • Author(s): Kawagishi, H.
  • Organizer: International Symposium on Natural Products Chemistry and Chemical Biology 2012
  • Place of Presentation: Hangzhou (China)
  • Invited
• [Presentation] Chemical Investigation of the Food-Poisoning Epidemics Caused by the Mushroom Pleurocybella porrigens
  • Author(s): Tomohiro Suzuki, Saori Omae, Motohiro Fujita, Tomohiro Asakawa, Toshiyuki Wakimoto, Kaoru Nagai, Hirofumi Hirai, Toshiyuki Kan, Hirokazu Kawagishi
  • Organizer: 13th International Conference on the Chemistry of Antibiotics and other bioactive compounds (ICCA)
  • Place of Presentation: 富士ビューホテル （山梨県）
• [Presentation] スギヒラタケレクチン（PPL）の糖鎖構造解析
  • Author(s): 荒井勇人、鈴木智大、崔宰熏、道羅英夫、平井浩文、舘野浩章、平林淳、小林夕香、河岸洋和
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学 （神奈川県）
• [Presentation] スギヒラタケ由来毒性物質の異種発現系の構築
  • Author(s): 亀澤哲弥、竹島愛乃、鈴木智大、崔宰熏、平井浩文、河岸洋和
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学 （神奈川県）
• [Presentation] キノコが産生するレクチンの特異な機能とその応用
  • Author(s): 河岸洋和
  • Organizer: 日本応用糖質科学会中部支部澱粉関連講演会
  • Place of Presentation: 愛知県産業労働センター （愛知県）
  • Invited
• [Presentation] スギヒラタケレクチンの異種発現解析
  • Author(s): 河岸洋和
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学
• [Presentation] スギヒラタケレクチン（PPL）の糖鎖構造解析
  • Author(s): 河岸洋和
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学
• [Presentation] 次世代シーケンサーを用いたスギヒラタケのゲノム及びトランスクリプトーム解析
  • Author(s): 河岸洋和
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学
• [Book] Lectins Methods and Protocols (2014)
  • Author(s): Kobayashi, Y and Kawagishi, H.
  • Total Pages: 613
  • Publisher: Springer