| Project/Area Number |
24248028
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Forest science
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| Research Institution | Kyoto University |
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Principal Investigator |
ISAGI Yuji 京都大学, (連合)農学研究科(研究院), 教授 (50325130)
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| Co-Investigator(Kenkyū-buntansha) |
TSUMURA Yoshihiko 筑波大学, 大学院生命環境科学研究科, 教授 (20353774)
UENO Saneyoshi 独立行政法人森林総合研究所, 研究員 (40414479)
SETOGUCHI Hiroaki 京都大学, 大学院人間・環境学研究科, 教授 (70206647)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKAGUCHI Shota 東京大学, 大学院総合文化研究科, 研究員 (50726809)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥35,750,000 (Direct Cost: ¥27,500,000、Indirect Cost: ¥8,250,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2012: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
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| Keywords | 適応進化 / 次世代シーケンサー / オーストラリア / 連鎖地図 / RAD-seq / 選択 / 進化 / 適応 / 系統解析 / RNA-seq / 種分化 / RNAseq |
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| Outline of Final Research Achievements |
We analyzed adaptation and speciation processes of Callitris species, which had been growing in various environments, by means of comparative analysis on samples from distinct populations, genotyping at thousands of loci. By evaluating phenotypic characteristics and genotypes of Callitris plants in a common garden, it was found that several morphological and physiological traits that relate to adaptation had genetic background. Candidate genomic regions relating the adaptive evolution were found by combining analysis of the information obtained by RAD-seq and RNA seq. By taking the advantage of haploid endosperm of gymnosperms, a high-density linkage map for Callitris was successfully constructed by genotyping only 1 mother tree and ca. 80 endosperms using a next generation sequencer.
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