Effects of stresses via CNS on fish energy metabolisms and immunological systems

• Project/Area Number: 24248037
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: USHIO Hideki 東京大学, 農学生命科学研究科, 教授 (50251682)
• Co-Investigator(Kenkyū-buntansha): HIRONO Ikuo 東京海洋大学, 大学院 (00270926) ; KONDO Hidehiro 東京海洋大学, 大学院 (20314635) ; NAGASAKA Reiko 東京海洋大学, 大学院 (90444132) ; KANEKO Gen 東京大学, 大学院 (30466809)
• Project Period (FY): 2012-10-31 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥42,120,000 (Direct Cost: ¥32,400,000、Indirect Cost: ¥9,720,000); Fiscal Year 2014: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000); Fiscal Year 2013: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2012: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
• Keywords: 水産業 / シグナル伝達 / 発現制御 / ストレス / 細胞・組織 / 脳・神経

Outline of Final Research Achievements

Evaluation methods of fish stress responses via central nervous system including visual stress, overcrowding stress, etc. were developed. Proteofragmentome analysis using NanoLC-MS/MS developed in this research suggested that proteolyses related to autophagy, proteasome system, and apoptosis occur in normal fish muscle tissue, and that the proteolyses are upregulated by the stresses. Immunological responses against the stresses were partly different among fish species. gamma Oryzanol is a stress-suppressor.

Research Products

• [Journal Article] Identification and gene expression profile analysis of a major type of lipoprotein lipase in adult medaka Oryzias latipes (2015)
  • Author(s): Lu Wang, Gen Kaneko, Shin-Ichiro Takahashi, Shugo Watabe and Hideki Ushio
  • Journal Title: Fisheries Science Volume: 81 Issue: 1 Pages: 163-173
  • DOI: 10.1007/s12562-014-0826-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Development of sandwich ELISA for detection and quantification of invertebrate major allergen tropomyosin by a monoclonal antibody (2014)
  • Author(s): Hong Zhang, Ying Lu, Hideki Ushio, Kazuo Shiomi
  • Journal Title: Food Chemistry Volume: 150 Pages: 151-157
  • DOI: 10.1016/j.foodchem.2013.10.154
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hormone-sensitive lipase in Japanese flounder Paralichthys olivaceus: putative function of the inclinator muscle of fin as a lipid storage site. (2014)
  • Author(s): Khieokhajonkhet A, Kaneko G, Ohara K, Shirakami H, Ushio H
  • Journal Title: Fish Sci Volume: 80 Issue: 2 Pages: 341-355
  • DOI: 10.1007/s12562-013-0695-5
  • Peer Reviewed
• [Journal Article] DNA microarray analysis on gene candidates possibly related to tetrodotoxin accumulation in pufferfish (2014)
  • Author(s): Holger Feroudj, Takuya Matsumoto, Yohei Kurosu, Gen Kaneko, Hideki Ushio, Katsuaki Suzuki, Hidehiro Kondo, Ikuo Hirono, Yuji Nagashima, Seiji Akimoto, Kazushige Usui, Shigeharu Kinoshita, Shuichi Asakawa, Masaaki Kodama, Shugo Watabe
  • Journal Title: Toxicon Volume: 77 Pages: 68-72
  • DOI: 10.1016/j.toxicon.2013.10.030
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] DNA microarray analysis on the genes differentially expressed in the liver of the pufferfish Takifugu rubripes following an intramuscular administration of tetrodotoxin. (2014)
  • Author(s): Matsumoto T, Feroudj H, Kikuchi R, Kawana Y, Kondo H, Hirono I, Aki S, Mochizuki T, Nagashima Y, Kaneko G, Ushio H, Kodama M, Watabe S.
  • Journal Title: Microarrays Volume: 3 Issue: 4 Pages: 226-244
  • DOI: 10.3390/microarrays3040226
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] EPA and DHA status of South Asian and White Canadians living in the National Capital Region of Canada (2014)
  • Author(s): Reiko Nagasaka, Claude Gagnon, Eleonora Swist, Isabelle Rondeau, Isabelle Massarelli, Winnie Cheung, Walisundera M.N. Ratnayake
  • Journal Title: Lipids Volume: 49 Issue: 10 Pages: 1057-1069
  • DOI: 10.1007/s11745-014-3942-3
  • Peer Reviewed / Open Access
• [Journal Article] Effects of short-term cold acclimation on FoF1-ATPase activity in skeletal muscle of red seabream Pagrus major (Temminck & Schlegel). (2014)
  • Author(s): Kaneko G, Sawada A, Ushio H, Watabe S.
  • Journal Title: Aquaculture Res Volume: 未定 Pages: 1-4
  • DOI: 10.1111/are.12132
  • Peer Reviewed
• [Journal Article] Molecular cloning and localization of GABAA receptor associated protein in the rotifer Brachionus plicatilis. (2014)
  • Author(s): Marcial HS, Suga K, Kinoshita S, Kaneko G, Hagiwara A, Watabe S
  • Journal Title: Int Rev Hydrobiol Volume: 99 Issue: 1-2 Pages: 188-197
  • DOI: 10.1002/iroh.201301720
  • Peer Reviewed
• [Journal Article] Proteins degradation value in cured meat product made from M. Cutaneous-omo brahialis muscle of bovine (2014)
  • Author(s): Ahhmed AM, Kaneko G, Ushio H, Karaman S, Inomata T, Sakata R, Yetim H
  • Journal Title: Eur Food Res Technol Volume: 238 Issue: 3 Pages: 387-396
  • DOI: 10.1007/s00217-013-2109-4
  • Peer Reviewed
• [Journal Article] Assessment of Commercial Quality Evaluation of Yellowfin Tuna <i>Thunnus albacares</i> Meat Based on Myoglobin Properties (2013)
  • Author(s): Nurilmala M, Ushio H, Kaneko G, Ochiai Y
  • Journal Title: Food Science and Technology Research Volume: 19 Issue: 2 Pages: 237-243
  • DOI: 10.3136/fstr.19.237
  • NAID: 10031163908
  • ISSN: 1344-6606, 1881-3984
  • Peer Reviewed
• [Journal Article] Comparison in taste and extractive components of boiled dorsal muscle and broth from half-smooth golden puffer Lagocephalus spadiceus caught in Japan with those of the same fish imported (2013)
  • Author(s): Hiroko Yamaguchi, Misako Nakaya,Gen Kaneko, Chie Yoneda, Toshitaka Mochizuki,Katsuya Fukami, Hideki Ushio,Shugo Watabe
  • Journal Title: Fish Sci Volume: 79 Issue: 2 Pages: 327-334
  • DOI: 10.1007/s12562-012-0585-2
  • NAID: 10031138671
  • Peer Reviewed
• [Journal Article] Differences in lipid distribution and expression of peroxisome proliferator-activated receptor gamma and lipoprotein lipase genes in torafugu and red seabream (2013)
  • Author(s): Kaneko G, Yamada T, Han Y, Hirano Y, Khieokhajonkhet A, Shirakami H, Nagasaka R, Kondo H, Hirono I, Ushio H, Watabe S
  • Journal Title: Gen Comp Endocrinol Volume: 184 Pages: 51-60
  • DOI: 10.1016/j.ygcen.2013.01.003
  • Peer Reviewed
• [Journal Article] Molecular phylogenetic relationship of Tetraodon pufferfish based on mitochondrial DNA analysis. (2013)
  • Author(s): Igarashi Y, Doi H, Yamanoue Y, Kinoshita S, Ishibashi T, Ushio H, Asakawa S, Nishida M, Watabe S.
  • Journal Title: Fisheries Science Volume: 79 Issue: 2 Pages: 243-250
  • DOI: 10.1007/s12562-013-0598-5
  • Peer Reviewed
• [Journal Article] Distribution of adipocyte-related cells in skeletal muscle of rainbow trout Oncorhynchus mykiss (2013)
  • Author(s): Yuna Han, Gen Kaneko, Reiko Nagasaka, Hidehiro Kondo, Ikuo Hirono, Shin-Ichiro Takahashi, Shugo Watabe, Hideki Ushio
  • Journal Title: Fisheries Science Volume: 79 Issue: 1 Pages: 143-148
  • DOI: 10.1007/s12562-012-0579-0
  • NAID: 10031138650
  • Peer Reviewed
• [Journal Article] Oxidized arachidonic acid and hexanal enhance mouse taste perception of monosodium glutamate (2013)
  • Author(s): Susumu Yamaguchi, Hidenori Fujiwara, Ikukazu Tashima, Hideki Ushio
  • Journal Title: Nutritional Neuroscience Volume: 16 Pages: 54-60
  • Peer Reviewed
• [Journal Article] Reliability of the Commercial Quality Evaluation on Yellowfin Tuna Thunnus albacares Meat (2013)
  • Author(s): Mala NURILMALA, Hideki USHIO, Gen KANEKO, Yoshihiro OCHIAI
  • Journal Title: Food Science and Technology Research Volume: in press
  • Peer Reviewed
• [Presentation] ヒラメ肝臓におけるペルフルオロオクタンスルホン酸の毒性影響 (2015)
  • Author(s): 佐藤根妃奈，陳盈光，浅川修一，潮 秀樹
  • Organizer: 日本水産学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-29
• [Presentation] リジン過剰飼料がニジマスの脂質代謝に及ぼす影響 (2015)
  • Author(s): 吉永葉月，金子元，潮秀樹，高橋伸一郎・，佐藤秀一
  • Organizer: 日本水産学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-28
• [Presentation] 魚類の筋タンパク質分解に関する研究 (2014)
  • Author(s): 長阪玲子，針ケ谷敦子，近藤秀裕，廣野育生，金子元，潮秀樹
  • Organizer: 日本水産学会
  • Place of Presentation: 福岡
  • Year and Date: 2014-09-21
• [Presentation] Analysis of tetrodotoxin dose dependent gene expression patterns in liver tissues of pufferfish Takifugu rubripes. (2014)
  • Author(s): Holger F，平野雪，佐藤根妃奈，松本拓也，金子元，陳盈光，浅川修一，渡部終五，潮秀樹.
  • Organizer: 日本水産学会
  • Place of Presentation: 福岡
  • Year and Date: 2014-09-20
• [Presentation] 魚類の短期ストレスが及ぼす筋代謝への影響 (2014)
  • Author(s): 金城春菜，石川雄樹，長阪玲子，近藤秀裕，廣野育生，金子元，潮秀樹
  • Organizer: 日本水産学会
  • Place of Presentation: 福岡
  • Year and Date: 2014-09-20
• [Presentation] Norie Kaneshige*, Hidehiro Kondo and Ikuo Hirono. Effect of temperature on innate immune response of Japanese flounder (Paralichthys olivaceus) (2014)
  • Author(s): Norie Kaneshige*, Hidehiro Kondo and Ikuo Hirono
  • Organizer: 7th International Symposium on Aquatic Animal Health
  • Place of Presentation: Portland
  • Year and Date: 2014-09-04
• [Presentation] 魚肉筋タンパク質の分解に関する研究 (2014)
  • Author(s): 長阪玲子，針ヶ谷敦子，近藤秀裕，廣野育生，金子元，潮秀樹
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学（北海道）
• [Presentation] γオリザノールの経口投与がニジマスのエネルギー代謝に及ぼす影響 (2014)
  • Author(s): Han Yuna，金子元，潮秀樹，長阪玲子，近藤秀裕，廣野育生
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学（北海道）
• [Presentation] 魚類のストレス応答が及ぼす筋代謝への影響 (2014)
  • Author(s): 金城春菜，石川雄樹，長阪玲子，近藤秀裕，廣野育生，金子元，潮秀樹
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学（北海道）
• [Presentation] 高密度飼育がトラフグのストレスおよび生体防御関連遺伝子群の発現動態におよぼす影響 (2014)
  • Author(s): 小林圭吾(海洋大)・潮秀樹(東大院農)・廣野育生・ 近藤秀裕
  • Organizer: 平成26年度 日本水産学会春季大会
  • Place of Presentation: 北海道大学（北海道）
• [Presentation] Biotechnology in food science. (2013)
  • Author(s): Kaneko G and Ushio H
  • Organizer: The First Turkish-International Circle's Workshop on Global Food Science and Technology
  • Place of Presentation: Erciyes University, Kayseri, Turkey
  • Invited
• [Presentation] Tissue distribution of lipid and transcript of hormone-sensitive lipase in Japanese flounder Paralichthys olivaceus (2013)
  • Author(s): Khieokhajonkhet A，Kaneko G，Ushio H
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 三重大学（三重県）
• [Presentation] トラフグおよびマダイにおける組織および血清リポタンパク質のTAG含量の比較 (2013)
  • Author(s): 平野雪，金子元，吉永葉月，潮秀樹
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 三重大学（三重県）
• [Presentation] 魚類の脂質代謝を制御するアミノ酸に関する研究 (2013)
  • Author(s): 大場萌未，金子元，潮秀樹，高橋伸一郎
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 三重県
• [Presentation] 魚類におけるTORシグナリングに影響を及ぼすアミノ酸に関する研究 (2013)
  • Author(s): 大原和幸，金子元，潮秀樹，高橋伸一郎
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 三重大学（三重県）
• [Presentation] トラフグ筋肉における成長ホルモンシグナルの解析 (2013)
  • Author(s): 平野雪，金子元，潮秀樹
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: ポートアイランド（兵庫県）
• [Presentation] トラフグ転写因子FoxO1の局在制御機構および標的遺伝子に関する研究 (2013)
  • Author(s): 金子雄亮，金子元，潮秀樹
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: ポートアイランド（兵庫県）
• [Presentation] The change of lipid metabolism related genes on lipid accumulated fish cells
  • Author(s): Kazuyuki Ohara, Gen Kaneko, Hideki Ushio, and Shi-Ichiro Takahashi
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡県博多区福岡国際会議場
• [Presentation] cDNA cloning, gene expression and nutritional regulation of the hormone sensitive lipase genes in red seabream (Pagrus major)
  • Author(s): Anurak Khieokhajonkhet, Gen Kaneko, Lu Wang, Muhammad Mehedi Hasan, Shugo Watabe and Hideki Ushio
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡県博多区福岡国際会議場
• [Presentation] In silico investigations of an endogenous cellulase from Teredo navalis
  • Author(s): Honein Karim, Kaneko Gen, Katsuyama Ichiro, Matsumoto Masaki, Kawashima Yukio, Yamada Masao, Ushio Hideki, Watabe Shugo
  • Organizer: 第35回日本分子生物学会大会
  • Place of Presentation: 福岡県博多区福岡国際会議場
• [Presentation] Whole transcriptome analysis of Teredo navalis in search of lignocellulose degrading genes
  • Author(s): Karim Honein, Gen Kaneko, Masao Yamada, Engkong Tan, Shuichi Asakawa, Hideki Ushio
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京都港区東京海洋大学
• [Presentation] ミドリフグ・ミオシン重鎖遺伝子TnMYHM2528-1の異なる塩分条件下での発現変動
  • Author(s): 五十嵐洋治，木下滋晴，土井啓行，石橋敏章，潮 秀樹，渡部終五
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京都港区東京海洋大学
• [Presentation] クロマグロ・ミオシン重鎖および転写因子Sox6遺伝子の筋肉部位による発現の違い
  • Author(s): 大西愛美，小山寛喜，木下滋晴，金子 元，浅川修一，室井洋佑，宮崎誠尚，渡部終五，潮 秀樹
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京都港区東京海洋大学
• [Presentation] エビ類の遊泳脚および幼生体からのミオシン重鎖遺伝子クローニング
  • Author(s): 小山寛喜，Sanit Piyapattanakorn，潮 秀樹，安元 剛，神保 充，渡部終五
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京都港区東京海洋大学
• [Presentation] 低アミノ酸飼料がニジマスの脂質代謝に及ぼす影響
  • Author(s): 吉永葉月，金子 元，潮 秀樹，高橋伸一郎，佐藤秀一
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京都港区東京海洋大学
• [Presentation] ゼブラフィッシュ培養細胞を利用したトラフグ転写因子FoxO1の機能解析
  • Author(s): 金子雄亮，金子 元，潮 秀樹
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京都港区東京海洋大学
• [Presentation] シログチおよびスケトウダラすり身ゲル形成能に及ぼす加熱温度履歴の影響について
  • Author(s): 松岡洋子，植木暢彦，鈴木大資，万建栄，潮 秀樹，渡部終五
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京都港区東京海洋大学
• [Presentation] ヒラメにおける低濃度および高濃度トリブチルスズの影響
  • Author(s): 佐藤根妃奈，金子 元，潮 秀樹，渡部終五
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京都港区東京海洋大学
• [Book] 増補改訂版魚類生理学の基礎 (2013)
  • Author(s): 潮 秀樹（曾田勝美，金子豊二編）
  • Total Pages: 264
  • Publisher: 恒星社厚生閣
• [Patent(Industrial Property Rights)] 飼育魚類の脂質含量の低減方法及びそのための飼料 (2015)
  • Inventor(s): 吉永，大場，潮，金子，高橋，佐藤，芳賀
  • Industrial Property Rights Holder: 東京大学，東京海洋大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-02-19
  • Overseas
• [Patent(Industrial Property Rights)] アディポネクチン分泌調節剤 (2014)
  • Inventor(s): 大原和幸，潮 秀樹，佐藤根妃奈(東京大学)，服部武史，上野友哉
  • Industrial Property Rights Holder: 東京大学，焼津水産化学工業
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-156767
  • Filing Date: 2014-07-31
• [Patent(Industrial Property Rights)] 飼育魚類の脂質含量の低減方法及びそのための飼料 (2014)
  • Inventor(s): 吉永葉月，大場萌未，金子元，潮秀樹，高橋伸一郎，芳賀穣，佐藤秀一
  • Industrial Property Rights Holder: 東京大学，東京海洋大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-033630
  • Filing Date: 2014-02-20
• [Patent(Industrial Property Rights)] 飼育魚類の筋肉内脂質含量増加方法及びそのための飼料 (2014)
  • Inventor(s): 大場萌未，吉永葉月，金子元，潮秀樹，高橋伸一郎，佐藤秀一
  • Industrial Property Rights Holder: 東京大学，東京海洋大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-03-14
  • Overseas
• [Patent(Industrial Property Rights)] 飼育魚類の筋肉内脂質含量増加方法及びそのための飼料 (2013)
  • Inventor(s): 大場萌未，吉永葉月，潮 秀樹，金子 元，高橋伸一郎，佐藤秀一
  • Industrial Property Rights Holder: 東京大学，東京海洋大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-02-21