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Cell-cell communications in developing skeletal muscle

Research Project

Project/Area Number 24248045
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionHokkaido University

Principal Investigator

NISHIMURA Takanori  北海道大学, (連合)農学研究科(研究院), 教授 (10237729)

Co-Investigator(Kenkyū-buntansha) TATSUMI Ryuichi  九州大学, 農学研究院, 准教授 (40250493)
OJIMA Koichi  独立行政法人農業, 食品産業技術総合研究機構・畜産草地研究所, 畜産物研究領域 (60415544)
HOSAKA Yoshinao  鳥取大学, 農学部共同獣医学科, 教授 (00337023)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥43,420,000 (Direct Cost: ¥33,400,000、Indirect Cost: ¥10,020,000)
Fiscal Year 2014: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2013: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2012: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Keywords骨格筋 / 筋細胞 / 脂肪細胞 / 神経細胞 / 生理活性因子 / 細胞間コミュニケーション / 細胞外マトリックス
Outline of Final Research Achievements

As skeletal muscle tissue consists of not only muscle cells but also other types of cells, including nerve cells and fat cells, the formation of skeletal muscle must be precisely regulated by these types of cells. Thus, we investigated the mechanisms of cell-cell communications in skeletal muscle. Myogenic cells secreted more than 400 proteins and approximately 8% of them were categorized as myokines. The levels of myokines that promote myotube formation peaked during early differentiation, whereas myokines contributing to repellent activity against nerve cells or suppression of adipogenesis decreased after differentiation. Myostatin secreted from myotubes suppressed the lipid accumulation in adipocytes. Some extracellular molecules modulated the action of myostatin to adipocytes. Satellite cells, resident myogenic stem cells, controlled neuritogenesis by up-regulating a secreted neural chemorepellent semaphorin 3A, which were induced by HGF that was secreted from macrophages.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (33 results)

All 2015 2014 2013 2012

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (22 results)

  • [Journal Article] Proteomic analysis of secreted proteins from skeletal muscle cells during differentiation2014

    • Author(s)
      Ojima K, Oe M, Nakajima I, Shibata M, Chikuni K, Muroya S, and Nishimura T.
    • Journal Title

      EuPA Open Proteomics

      Volume: 5 Pages: 1-9

    • DOI

      10.1016/j.euprot.2014.08.001

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cold Exposure Increases Slow-Type Myosin Heavy Chain 1 (MyHC1) Composition of Soleus Muscle in Wistar Rats2014

    • Author(s)
      Wataru Mizunoya, Yohei Iwamoto, Yusuke Sato, Ryuichi A Tatsumi, Yoshihide Ikeuchi
    • Journal Title

      Animal Science Journal

      Volume: 85 Issue: 3 Pages: 293-304

    • DOI

      10.1111/asj.12143

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Supplementary immunocytochemistry of hepatocyte growth factor production in activated macrophages early in muscle regeneration.2014

    • Author(s)
      Sawano S, Suzuki T, Do MKQ, Ohtsubo H, Mizunoya W, Ikeuchi Y, Tatsumi R.
    • Journal Title

      Animal Science Journal

      Volume: 85 Issue: 12 Pages: 994-1000

    • DOI

      10.1111/asj.12264

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 筋幹細胞が分泌するSema3Aによる筋線維型自律制御機構2014

    • Author(s)
      鈴木貴弘, 大屋雄暉, 尾嶋孝一, 大坪秀明, 小宮佑介, 水野谷航, 中村真子, 池内義秀, 古瀬充宏, 辰巳隆一
    • Journal Title

      栄養生理研究会報

      Volume: 58 Pages: 19-25

    • NAID

      40020020774

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Preliminary Time-Course Study of Antiinflammatory Macrophage lnfiltration in Crush-Injured Skeletal Muscle.2013

    • Author(s)
      Shono J. Sakaguchi S. Suzuki T, et al.
    • Journal Title

      Animal Science Journal

      Volume: 84 Issue: 11 Pages: 744-750

    • DOI

      10.1111/asj.12105

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Laminin binds to myostatin and attenuates its signaling2013

    • Author(s)
      Naofumi YASAKA
    • Journal Title

      Animal Science Jornal

      Volume: 84 Issue: 9 Pages: 663-668

    • DOI

      10.1111/asj.12052

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Decorin activates Akt down stream of IGF-IR and promotes myoblast differentiation2013

    • Author(s)
      Keisuke SUZUKI
    • Journal Title

      Animal Science Jornal

      Volume: 84 Issue: 9 Pages: 669-668

    • DOI

      10.1111/asj.12055

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite ceels in vitro toward understanding its role in modulating2013

    • Author(s)
      Suzuki T et aj.
    • Journal Title

      ternational Journal of Biochemistry and Cell Biolo

      Volume: 45 Issue: 2 Pages: 476-482

    • DOI

      10.1016/j.biocel.2012.10.003

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Satellite ceels produce neural chemorepellent semaphorin 3A upon muscle injury.2013

    • Author(s)
      Sato Y, et al.
    • Journal Title

      Animal Science Journal

      Volume: 84 Issue: 2 Pages: 185-189

    • DOI

      10.1111/asj.12014

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Myostatin regulates proliferation and extracellular matrix mRNA expression in NIH3T3 fibroblasts2012

    • Author(s)
      Hosaka Yoshinao
    • Journal Title

      Biomedical Research

      Volume: 33 Pages: 355-361

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle.2012

    • Author(s)
      DO MK, et al.
    • Journal Title

      Animal Science Journal

      Volume: 83 Issue: 10 Pages: 712-717

    • DOI

      10.1111/j.1740-0929.2012.01057.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 骨格筋細胞が増殖から分化に至る過程で分泌する因子のプロテオーム解析2015

    • Author(s)
      尾嶋孝一, 大江美香, 中島郁世, 室谷進, 西邑隆徳
    • Organizer
      日本畜産学会第119回大会
    • Place of Presentation
      宇都宮市(宇都宮大学)
    • Year and Date
      2015-03-27 – 2015-03-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] 筋芽細胞の増殖時・分化時におけるSema3AとPax7発現の相互作用2015

    • Author(s)
      木 欄, 尾嶋孝一, 中村真子, 水野谷航, 辰巳隆一, 池内義秀
    • Organizer
      日本畜産学会第119回大会
    • Place of Presentation
      宇都宮大学(宇都宮市)
    • Year and Date
      2015-03-27 – 2015-03-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Sema3A ligand secreted from satellite cells promotes aneural nAChR clustering2015

    • Author(s)
      ド マイコイ, 水野谷航, 尾嶋孝一, 中村真子, 池内義秀, 辰巳隆一
    • Organizer
      日本畜産学会第119回大会
    • Place of Presentation
      宇都宮大学(宇都宮市)
    • Year and Date
      2015-03-27 – 2015-03-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] 筋幹細胞特異的 Sema3A cKO マウスの解析2015

    • Author(s)
      鈴木貴弘, 西松伸一郎, 寺田久美子, 片瀬直樹, 濃野 勉, 水野谷航, 池内義秀, 辰巳隆一
    • Organizer
      日本畜産学会第119回大会
    • Place of Presentation
      宇都宮大学(宇都宮市)
    • Year and Date
      2015-03-27 – 2015-03-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Morphological study of muscle regeneration following glycerol injury in mice and rats2015

    • Author(s)
      Miyake Yae, Hosaka Yoshinao Z.
    • Organizer
      The 5th Congress of Asian Association of Veterinary Anatomist
    • Place of Presentation
      Bali, Indonesia
    • Year and Date
      2015-02-12 – 2015-02-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dynamics of secreted proteins from skeletal muscle cells during muscle differentiation2014

    • Author(s)
      Ojima K, Oe M, Nakajima I, Muroya S, and Nishimura T.
    • Organizer
      2014 Annual Meeting of the American Society for Cell Biology
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2014-12-06 – 2014-12-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Anti-inflammatory macrophages implicate in regenerative moto-neuritogenesis, by promoting myoblast migration and Sema3A expression2014

    • Author(s)
      Sakaguchi, S., Shono, J., Suzuki, T., Sawano, S., Anderson, J.E., Do, M.-K.Q., Ohtsubo, H., Mizunoya, W., Nakamura, M., Furuse, M., Ikeuchi, Y., and Tatsumi, R.
    • Organizer
      Animal Science Congress 2014 of the Asian-Australian Association of Animal Production Societies (AAAP)
    • Place of Presentation
      Yogyakarta, Indonesia
    • Year and Date
      2014-11-10 – 2014-11-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Functional interaction between Sema3A and Pax7 in proliferation and differentiation of myoblasts2014

    • Author(s)
      Mulan, Nakamura, M., Suzuki, T., Mizunoya, W., Ojima, K., Tatsumi, R. and Ikeuchi, Y.
    • Organizer
      International Symposium on Agriculture, Forestry, Environment and Life Sciences in Asia, 2014
    • Place of Presentation
      Chuncheon, Gangwon, Korea
    • Year and Date
      2014-10-28 – 2014-10-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Muscle regeneration dynamics: satellite cells may do more2014

    • Author(s)
      Tatsumi, R., Anderson, J.E. and Allen, R.E.
    • Organizer
      A Meeting-of-Minds Muscle Symposium
    • Place of Presentation
      Winnipeg, MB R3T 2N2, Canada
    • Year and Date
      2014-09-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] M2 macrophages may implicate in regenerative moto-neuritogenesis, by promoting myoblast migration and Sema3A expression.2014

    • Author(s)
      Tatsumi, R., Sakaguchi, S., Shono, J., Suzuki, T., Sawano, S., Anderson, J.E., Do, M.-K.Q., Ohtsubo, H., Mizunoya, W., Nakamura, M., Furuse, M., and Ikeuchi, Y.
    • Organizer
      2014 FASEB Science Research Conference on “Skeletal Muscle Satellite and Stem Cells”
    • Place of Presentation
      Steamboat Springs, CO, USA
    • Year and Date
      2014-07-20 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] APOBEC2 deficiency up-regulates myoblast differentiation2014

    • Author(s)
      Ohtsubo, H., Sato, Y., Suzuki, T., Mizunoya, W., Nakamura, M., Tatsumi, R., and Ikeuchi, Y.
    • Organizer
      2014 FASEB Science Research Conference on “Skeletal Muscle Satellite and Stem Cells”
    • Place of Presentation
      Steamboat Springs, CO, USA
    • Year and Date
      2014-07-20 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Muscle cells suppress the differentiation of adipocytes vie myostatin in co-culture system2013

    • Author(s)
      Matsumoto, S., Wakamatsu, J., & Nishimura, T.
    • Organizer
      The 2013 Annual Meeting The American Society for Cell Biology (ASCB)
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2013-12-14 – 2013-12-18
    • Related Report
      2013 Annual Research Report
  • [Presentation] Semaphorin 3A secreted from myogenic stem cells promotes slow-twitch muscle fiber generation2013

    • Author(s)
      Takahiro Suzuki, Koichi Ojima, Mai-Khoi Q. Do, Minako Hara, Wataru Mizunoya, Mako Nakamura, Yoshihide Ikeuchi, Judy E. Andeson, Ryuichi Tatsumi
    • Organizer
      2013 EMBO Workshop on “Semaphorin Function and Mechanism in Action”
    • Place of Presentation
      Cemay-la-Ville, France
    • Year and Date
      2013-10-30
    • Related Report
      2013 Annual Research Report
  • [Presentation] 筋肥大・再生における筋幹細胞・運動神経末端・マクロファージのコミュニケーションダイナミクス2013

    • Author(s)
      辰巳 隆一
    • Organizer
      第43回日本栄養・食糧学会 北海道支部会シンポジウム「「組織の機能を支える細胞間クロストーク」
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2013-10-26
    • Related Report
      2013 Annual Research Report
  • [Presentation] Muscle regeneration dynamics mediated by resident myogenic stem cells: a possible implication in moto-neuritogenesis and fiber-type regulation2013

    • Author(s)
      Ryuichi Tatsumi, Judy E. Andeson, Ronald E. Allen
    • Organizer
      Tucson Symposium 2013 on "The Biology of Muscle Growth and Repair"
    • Place of Presentation
      Tucson, USA
    • Year and Date
      2013-09-27
    • Related Report
      2013 Annual Research Report
  • [Presentation] 筋幹細胞由来の分泌因子Sema3Aによる筋線維型自律制御機構の解明2013

    • Author(s)
      鈴木貴弘, 尾嶋孝一, 大屋雄暉, 大坪秀明, 水野谷 航, 中村 真子, 池内 義秀, 辰巳 隆一
    • Organizer
      第117回日本畜産学会大会
    • Place of Presentation
      新潟大学(新潟市)
    • Year and Date
      2013-09-09
    • Related Report
      2013 Annual Research Report
  • [Presentation] 筋肉内結合組織を培養基質とした脂肪細胞培養系の確立2013

    • Author(s)
      雷 暁瑩,鈴木健司,若松純一,西邑隆徳
    • Organizer
      第117回日本畜産学会大会
    • Place of Presentation
      新潟大学(新潟市)
    • Year and Date
      2013-09-09
    • Related Report
      2013 Annual Research Report
  • [Presentation] 筋細胞との共培養が脂肪前駆細胞の分化に及ぼす影響2013

    • Author(s)
      松本しおり
    • Organizer
      第116回日本畜産学会大会
    • Place of Presentation
      安田女子大学、広島市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Syndecan-3 may implicate in regulation of neural chemorepellent Sema3A expression in satellite cells2013

    • Author(s)
      ド・マイコイ
    • Organizer
      第116回日本畜産学会大会
    • Place of Presentation
      安田女子大学、広島市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 筋幹細胞による筋線維型の自律制御機構2013

    • Author(s)
      鈴木貴弘
    • Organizer
      第116回日本畜産学会大会
    • Place of Presentation
      安田女子大学、広島市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Heparan/chondroitin sulfate chains may mediate HGF/FGF2-induced up-regulation of neural chemorepellent Sema3A in satellite cell cultures2012

    • Author(s)
      Do, M.Q.
    • Organizer
      2012 FASEB Science Research Conference on “Skeletal Muscle Satellite & Stem Cells”
    • Place of Presentation
      Renaissance Tuscany Il Ciocco Resort & Spa, Barga, Lucca, Italy
    • Related Report
      2012 Annual Research Report
  • [Presentation] Semaphorin 3A regulates the early differentiation of satellite cells2012

    • Author(s)
      Suzuki, T.
    • Organizer
      2012 FASEB Science Research Conference on “Skeletal Muscle Satellite & Stem Cells”
    • Place of Presentation
      Renaissance Tuscany Il Ciocco Resort & Spa, Barga, Lucca, Italy
    • Related Report
      2012 Annual Research Report

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Published: 2013-05-15   Modified: 2019-07-29  

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