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Identification of the factor in the upstream of the cascade of regulation of follicular growth and enhancement of OPU technology by using of the identified factor

Research Project

Project/Area Number 24248047
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionNational Livestock Breeding Center, Incorporated Administrative Agency (2013-2015)
Tohoku University (2012)

Principal Investigator

Sato Eimei  独立行政法人家畜改良センター, その他部局等, 理事長 (80093243)

Co-Investigator(Kenkyū-buntansha) TANEMURA Kentaro  東北大学, 大学院農学研究科, 教授 (20332322)
HOSHINO Yumi  広島大学, 大学院生命圏科学研究科, 助教 (10451551)
HASHIYADA Yutaka  独立行政法人家畜改良センター, 企画調整部, 技術専門役 (50462536)
YAMANOUCHI Tasayuki  独立行政法人家畜改良センター, 企画調整部, 係長 (30713581)
Project Period (FY) 2012-10-31 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥42,510,000 (Direct Cost: ¥32,700,000、Indirect Cost: ¥9,810,000)
Fiscal Year 2015: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2014: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2013: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2012: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Keywords卵胞発育 / 血管増殖 / OPU / FGT-OPU / IVMFC / 和牛 / 卵母細胞 / 体外成熟 / 体外受精 / 牛 / 黒毛和種 / 褐毛和種 / 排卵誘発 / FAK / 卵巣 / OPU-IVP / FSH / 体内成熟卵子 / 未成熟卵子 / 卵子卵丘複合体 / 体内成熟卵 / 体外成熟(IVM) / 抗酸化作用 / オートファジー / LC3 / コネキシン43 / 卵胞発育調節因子 / グリコサミノグリカン / OPU
Outline of Final Research Achievements

Practical OPU technology in wagyu beef cattle has been established by improvement of bovine OPU technology using follicular growth-stimulating factors. The recovery-rate of oocytes from medium and large sized follicles has been improved by the stimulation of the factor identified in this study. The percent of embryo developed to blastocyst by IVMFC is also improved in this study. These improved results were obtained in brown beef Wagyu as well as black beef Wagyu. FGT-OPU method developed in this study showed the increase of healthy oocytes and fertilization of oocytes and development of fertilized eggs. The original culture methods(hanging drop) was established for analyzing the biological safety of the agricultural chemicals in oocyte maturation, and clarified that some of the agricultural chemicals showed the harmful activity in spindle formation.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (43 results)

All 2016 2015 2014 2013 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results,  Open Access: 1 results) Presentation (29 results) (of which Invited: 4 results) Book (2 results)

  • [Journal Article] Distribution of the sex chromosome during mouse spermatogenesis in testis tissue sections2015

    • Author(s)
      Otaka K, Hiradate Y, Kobayashi N, Shirakata Y, Tanemura K.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 61 Issue: 5 Pages: 375-381

    • DOI

      10.1262/jrd.2015-013

    • NAID

      130005104108

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The effects analysis of two neonicotinoid insecticides on <i>in vitro</i> maturation of porcine oocytes using hanging drop monoculture method2015

    • Author(s)
      Ishikawa S, Hiraga K, Hiradate Y, Tanemura K.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 77 Issue: 6 Pages: 725-728

    • DOI

      10.1292/jvms.15-0008

    • NAID

      130005085865

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intraovarian control of selective follicular growth and induction of oocyte maturation in mammals2015

    • Author(s)
      Sato,E
    • Journal Title

      Proceedings of the Japan Academy, Series B

      Volume: 91 Issue: 3 Pages: 76-91

    • DOI

      10.2183/pjab.91.76

    • NAID

      130004802304

    • ISSN
      0386-2208, 1349-2896
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of focal adhesion kinase in mouse cumulus-oocyte complexes and effect of phosphorylation at Tyr397 on cumulus expansion2015

    • Author(s)
      Ohtake J, Sakurai M, Hoshino Y, Tanemura K, Sato E.
    • Journal Title

      Molecular Reproduction and Development

      Volume: 82(3) Issue: 3 Pages: 218-231

    • DOI

      10.1002/mrd.22464

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Histone H4 Modification During Mouse Spermatogenesis2014

    • Author(s)
      Shirakata Y, Hiradate Y, Inoue H, Sato E, Tanemura K.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 60 Issue: 5 Pages: 383-387

    • DOI

      10.1262/jrd.2014-018

    • NAID

      130004677398

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 卵成熟における紡錘体の形成メカニズムと加齢による紡錘体への影響2014

    • Author(s)
      星野由美
    • Journal Title

      日本IVF学会誌

      Volume: 17(2) Pages: 10-15

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of single-oocyte culture system on in vitro maturation and developmental competence in mice.2014

    • Author(s)
      Manami Nishio, Yumi Hoshino, Kentaro Tanemura, Eimei Sato
    • Journal Title

      Reproductive Medicine and Biology

      Volume: 13 Issue: 3 Pages: 153-159

    • DOI

      10.1007/s12522-014-0177-1

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Site-specific phosphorylation of Tau protein is associated with deacetylation of microtubules in mouse spermatogenic cells during meiosis.2014

    • Author(s)
      種村 健太郎
    • Journal Title

      FEBS Lett.

      Volume: In press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative analysis in LC3-II protein in vitro maturation of porcine oocyte.2013

    • Author(s)
      佐藤 英明
    • Journal Title

      Zygote

      Volume: 12 Issue: 3 Pages: 1-7

    • DOI

      10.1017/s0967199413000269

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution and association of mTOR with its cofactors2013

    • Author(s)
      Yuhei KOGASAKA, Yumi HOSHINO, Yuki HIRADATE, Kentaro TANEMURA, Eimei SATO
    • Journal Title

      raptor and rictor, in cumulus cells and oocytes during meiotic maturation in mice, Molecular Reproduction and Development

      Volume: Vol.80, No.4 Issue: 4 Pages: 334-348

    • DOI

      10.1002/mrd.22166

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Restoring of corpus luteum angiogenesis in immature hypothyroid rdw rats after thyroxine treatment : Morphological and Molecular evidences in press, 20122013

    • Author(s)
      佐藤英明
    • Journal Title

      Theriogenology

      Volume: 79 Issue: 1 Pages: 116-126

    • DOI

      10.1016/j.theriogenology.2012.09.016

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] L-carnitine improves hydrogen peroxide-induced impairment of nuclear maturation in porcine oocytes2013

    • Author(s)
      佐藤英明
    • Journal Title

      Animal Science Journal

      Volume: 印刷中 Issue: 5 Pages: 395-402

    • DOI

      10.1111/asj.12016

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 着床しやすい胚の特徴と選び方2016

    • Author(s)
      星野由美
    • Organizer
      第39回家畜診療技術研究会
    • Place of Presentation
      東広島(広島県)
    • Year and Date
      2016-03-10
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ウシ卵子の成熟培地へのプロジェステロン添加が体外受精後の胚発生に及ぼす影響2016

    • Author(s)
      山之内忠幸、松田秀雄、大竹正樹、後藤由希、小林修司、橋谷田豊
    • Organizer
      第31回東日本受精卵移植技術研究会大会
    • Place of Presentation
      石川県立音楽堂(石川県金沢市)
    • Year and Date
      2016-02-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] Effect of corpus luteum existence for the duration of follicular growth on bovine oocyte development competence2016

    • Author(s)
      Tadayuki Yamanouchi, Hideo Matsuda, Masaki Ohtake, Yuki Goto, Yoshio Aikawa, Syuji Kobayashi and Yutaka Hashiyada
    • Organizer
      42nd IETS Annual Conference
    • Place of Presentation
      アメリカ、ルイビル
    • Year and Date
      2016-01-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] マウス卵成熟過程におけるTauの発現とリン酸化パターンの解析2015

    • Author(s)
      種村健太郎
    • Organizer
      第108回日本繁殖生物学会大会
    • Place of Presentation
      宮崎大学農学部(宮崎県宮崎市
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 成熟培地へのFSH,EGF様ペプチドおよびdbcAMPの添加がウシ体外受精胚作出に及ぼす影響について2015

    • Author(s)
      山之内忠幸、相川芳雄、松田秀雄、後藤由希、大竹正樹、小林修司、橋谷田豊
    • Organizer
      第108回日本繁殖生物学会大会
    • Place of Presentation
      宮崎大学農学部(宮崎県宮崎市)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 褐毛和種および黒毛和種の体内成熟卵子における体外受精後の卵割状況の比較2015

    • Author(s)
      山之内忠幸、松田秀雄、大竹正樹、相川芳雄、後藤由希、小林修司、橋谷田豊
    • Organizer
      第22回日本胚移植研究会大会
    • Place of Presentation
      高知大学農学部(高知県南国市)
    • Year and Date
      2015-08-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] マウス卵子および初期胚におけるPin1の局在解析2015

    • Author(s)
      星野由美
    • Organizer
      第56回日本卵子学会
    • Place of Presentation
      栃木県総合文化センター(栃木県宇都宮市
    • Year and Date
      2015-05-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] マウス卵子減数分裂時の Tau の発現とそのリン酸化動態2015

    • Author(s)
      平舘 裕希、井上 弘貴、小倉 淳郎、種村 健太郎
    • Organizer
      日本畜産学会 第119回大会
    • Place of Presentation
      宇都宮大学(栃木県宇都宮市)
    • Year and Date
      2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] ビスフェノール類の慢性投与が精子発生過程および精子に与える影響解析2015

    • Author(s)
      白形 芳樹、小林 記緒、平舘 裕希、種村 健太郎
    • Organizer
      日本畜産学会 第119回大会
    • Place of Presentation
      宇都宮大学(栃木県宇都宮市)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] MeCP2 タンパクのマウス精子における発現解析2015

    • Author(s)
      小林 記緒、大高 康佑、白形 芳樹、平舘 裕希、種村 健太郎
    • Organizer
      日本畜産学会 第119回大会
    • Place of Presentation
      宇都宮大学(栃木県宇都宮市)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 褐毛和種不妊牛からのOPU-IVPによる効率的胚作出の検討2015

    • Author(s)
      山之内忠幸、松田秀雄、大竹正樹、相川芳雄、渡邉一博、齋藤邦彦、星一浩、後藤由希、小林修司、橋谷田豊
    • Organizer
      第30回東日本家畜受精卵移植技術研究会大会
    • Place of Presentation
      秋田キャッスルホテル(秋田県秋田市)
    • Year and Date
      2015-02-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of in vivo matured oocytes collected from Japanese black cattle stimulated with different duration of follicular growth2015

    • Author(s)
      Tadayuki Yamanouchi, Hideo Matsuda, Masaki Ohtake, Yoshio Aikawa, Syuji Kobayashi, Kei Imai, Yutaka Hashiyada
    • Organizer
      41st IETS Annual Conference
    • Place of Presentation
      ヴェルサイユ(フランス)
    • Year and Date
      2015-01-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] ネオニコチノイド系農薬による中枢神経影響解析および生殖機能影響解析2014

    • Author(s)
      種村 健太郎、菅野 純
    • Organizer
      環境ホルモン学会第17回研究発表会
    • Place of Presentation
      東京大学山上会館(東京都文京区)
    • Year and Date
      2014-12-10
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Hanging Drop 法を用いた体外成熟培養系におけるマウス卵母細胞へのアセタミプリド(ACE)曝露影響2014

    • Author(s)
      猪股大貢, 種村健太郎
    • Organizer
      環境ホルモン学会第17回研究発表会
    • Place of Presentation
      東京大学山上会館(東京都文京区)
    • Year and Date
      2014-12-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 幼若期雄マウスへのアセフェート投与による遅発生殖機能影響解析2014

    • Author(s)
      植松未知, 井上弘貴, 斉藤洋克, 種村健太郎
    • Organizer
      環境ホルモン学会第17回研究発表会
    • Place of Presentation
      東京大学山上会館(東京都文京区)
    • Year and Date
      2014-12-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウスへのネオニコチノイド系農薬アセタミプリド単回曝露による遅発中枢影響の性差2014

    • Author(s)
      平賀 孔、種村 健太郎
    • Organizer
      環境ホルモン学会第17回研究発表会
    • Place of Presentation
      東京大学山上会館(東京都文京区)
    • Year and Date
      2014-12-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 幼若期雄マウスへのぺルメトリン投与による遅発生殖機能影響解析2014

    • Author(s)
      斉藤洋克, 植松未知, 白形芳樹, 種村健太郎
    • Organizer
      環境ホルモン学会第17回研究発表会
    • Place of Presentation
      東京大学山上会館(東京都文京区)
    • Year and Date
      2014-12-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 黒毛和種におけるOPU由来体内成熟卵子の体外受精成績2014

    • Author(s)
      山之内忠幸、松田秀雄、大竹正樹、相川芳雄、小林修司、橋谷田豊
    • Organizer
      第21回日本胚移植研究会大会
    • Place of Presentation
      岡山大学50周年記念館(岡山県岡山市)
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 幼若期雄マウスへのペルメトリン暴露による遅発性生殖機能影響解析2014

    • Author(s)
      斉藤 洋克、植松 未知、白形 芳樹、種村 健太郎
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 体外成熟培養系における卵母細胞へのアセタミプリド(ACE)曝露影響2014

    • Author(s)
      猪股 大貢、星野 由美、種村 健太郎
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] ブタ成熟卵母細胞のガラス化凍結保存におけるラクトフェリンの有用性評価2014

    • Author(s)
      根岸 真奈美、石川 禎将、平賀 孔、種村 健太郎
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2014-09-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウス卵子減数分裂過程におけるタウ動態2014

    • Author(s)
      平舘 裕希、井上 弘貴、小倉 淳郎、種村 健太郎
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2014-09-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Inhibition of mTOR signaling induces cumulus expansion and stimulates meiotic maturation of oocytes in mice.2014

    • Author(s)
      Yumi Hoshino
    • Organizer
      The World Congress of Reproductive Biology 2014
    • Place of Presentation
      エジンバラ(スコットランド)
    • Year and Date
      2014-09-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] ウシ体外作出胚の受胎性に関わる品質分析パラメーターによる体外成熟培地へのFSH,EGFおよびジブチリルcAMP添加効果の評価2014

    • Author(s)
      山之内忠幸、松田秀雄、相川芳雄、大竹正樹、小林修司、今井敬、橋谷田豊
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      帯広畜産大学(北海道帯広市)
    • Year and Date
      2014-08-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Evaluation of maturation competence of metaphase II oocytes in mice based on the distance between pericentriolar materials of meiotic spindle.2014

    • Author(s)
      Yumi Hoshino
    • Organizer
      The 37th Naito Conference: Bioimaging - a paradigm shift for the life sciences
    • Place of Presentation
      ヒルトンニセコビレッジ(北海道虻田郡ニセコ町)
    • Year and Date
      2014-07-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウスへのネオニコチノイド系農薬アセタミプリド単回曝露による遅発中枢影響の性差2014

    • Author(s)
      平賀 孔、種村 健太郎
    • Organizer
      第41回日本毒性学会学術年会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県神戸市)
    • Year and Date
      2014-07-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] Spindle formation-related proteins responsible for oocyte quality.2014

    • Author(s)
      Yumi Hoshino
    • Organizer
      The 3rd Korea-China-Japan Joint Symposium on Animal Reproduction
    • Place of Presentation
      済州島(韓国)
    • Year and Date
      2014-07-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 卵成熟機構からみたARTに応用できる基礎研究

    • Author(s)
      星野 由美
    • Organizer
      第31回日本受精着床学会総会・学術集会
    • Place of Presentation
      大分(別府)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ハンギングドロップ(HD)法を用いたブタ卵母細胞体外成熟培地への抗酸化物質添加の検討

    • Author(s)
      種村健太郎
    • Organizer
      第106回日本繁殖生物学会大会
    • Place of Presentation
      東京 農工大学農学部府中キャンパス
    • Related Report
      2013 Annual Research Report
  • [Book] 哺乳動物の発生工学、4:胚の全胚培養2014

    • Author(s)
      種村 健太郎
    • Total Pages
      200
    • Publisher
      朝倉書店
    • Related Report
      2013 Annual Research Report
  • [Book] シリーズ<家畜の科学>2 ブタの科学、13.1:ブタをめぐる最近のトピックス2014

    • Author(s)
      種村 健太郎
    • Total Pages
      193
    • Publisher
      朝倉書店
    • Related Report
      2013 Annual Research Report

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Published: 2012-11-27   Modified: 2019-07-29  

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