A molecular study on skin barrier dysfunction in atopic dermatitis
| Project/Area Number |
24248055
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
MATSUDA Hiroshi 東京農工大学, (連合)農学研究科(研究院), 教授 (80145820)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Akane 東京農工大学, 大学院農学研究院, 教授 (80418673)
OHMORI Keitaro 東京農工大学, 大学院農学研究院, 講師 (20466915)
MATSUDA Akira 東京農工大学, 大学院農学研究院, 助教 (90613969)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥32,370,000 (Direct Cost: ¥24,900,000、Indirect Cost: ¥7,470,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2013: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2012: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
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| Keywords | トランスレーショナルリサーチ / 動物 / 酵素 / ストレス |
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| Outline of Final Research Achievements |
Raised skin pH may result in enzymatic activation of kallikrein 5 (KLK5), a serine protease thought to play a crucial role in skin barrier destruction. Although skin surface pH is increased in patients with atopic dermatitis (AD), contribution of pH to initiation of AD has been unclear. By using NC/Tnd mice, a model for human AD, skin pH and transepidermal water loss (TEWL), as well as KLK5, protease-activated receptor 2 (PAR2), and thymic stromal lymphopoietin (TSLP) were measured. Acidification of the skin reduced KLK5 activity in the epidermis and ameliorated the eczema. In contrast, alkalization of the skin increased epidermal expression of KLK5 and TEWL, resulting in development of AD, even in SPF NC/Tnd mice without AD. Skin surface pH modulates KLK5 production, which might directly be responsible for barrier dysfunction, itch sensation, and allergic inflammation by activating a PAR2 and TSLP cascade in the epidermis.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Dihomo-γ-linolenic acid prevents the development of atopic dermatitis through prostaglandin D1 production in NC/Tnd mice.2015
Author(s)
Amagai Y, Oida K, Matsuda A, Jung K, Kakutani S, Tanaka T, Matsuda K, Jang H, Ahn G, Xia Y, Kawashima H, Shibata H, Matsuda H, Tanaka A.
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Journal Title
J. Dermatol. Sci.
Volume: In press
Issue: 1
Pages: 30-37
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Ultra-pure soft water improves skin barrier function in children with atopic dermatitis: a randomized, double-blind, placebo-controlled, crossover pilot study.2014
Author(s)
Togawa Y, Kambe N, Shimojo N, Nakano T, Sato Y, Mochizuki H, Tanaka A, Matsuda H, Matsue H.
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Journal Title
J. Dermatol. Sci.
Volume: 76
Issue: 3
Pages: 269-271
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Daily intake of Jeju groundwater improves the skin condition of the model mouse for human atopic dermatitis.2013
Author(s)
Tanaka A, Jung K, Matsuda A, Jang H, Kajiwara N, Amagai Y, Oida K, Ahn G, Ohmori K, Kang KG, Matsuda H
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Journal Title
Journal of Dermatology
Volume: 40
Issue: 3
Pages: 193-200
DOI
Related Report
Peer Reviewed
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[Journal Article] Supplementation of the fermented soy product ImmuBalanceTM effectively reduces itching behavior of atopic NC/Tnd mice2012
Author(s)
Matsuda A, Tanaka A, Pan W, Okamoto N, Oida K, Kingyo N, Amagai Y, Xia Y, Jang H, Nishikawa S, Kajiwara N, Ahn G, Ohmori K, Matsuda H
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Journal Title
J. Dermatol. Sci
Volume: 67(2)
Issue: 2
Pages: 130-139
DOI
Related Report
Peer Reviewed
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