Defence mechanism against environmental chemicals in livestock animals
Project/Area Number |
24248056
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Boundary agriculture
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Research Institution | Hokkaido University |
Principal Investigator |
ISHIZUKA MAYUMI 北海道大学, (連合)獣医学研究科, 教授 (50332474)
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Co-Investigator(Kenkyū-buntansha) |
IKENAKA Yoshinori 北海道大学, 大学院獣医学研究科, 准教授 (40543509)
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Co-Investigator(Renkei-kenkyūsha) |
TSUBOTA Toshio 北海道大学, 大学院獣医学研究科, 教授 (10207441)
KONNAI Satoru 北海道大学, 大学院獣医学研究科, 准教授 (40396304)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥45,240,000 (Direct Cost: ¥34,800,000、Indirect Cost: ¥10,440,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2012: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
|
Keywords | シトクロムP450 / グルクロン酸抱合 / 環境汚染 / 産業動物 / グルクロン酸抱合酵素 / 獣医学 / グルクロン酸転移酵素 / 硫酸転移酵素 / グルタチオン転移酵素 |
Outline of Final Research Achievements |
In livestock animals, we identified the enzymatic characters of cytochrome P450 as typical phase I enzyme for xenobiotics. We also analyzed glucronyltrasnferase and sulfotransferase dependent metabolites in urine samples from mammalians including livestock animals, to elucidate the ability of xenobiotic conjugating detoxification. In field surveillance at Asia and African countries, heavy metals are major accumulated environmental pollutants in cattle, and the accumulating pattern is unique in ruminants. In case of poultry species, we clarify the expressed cytochrome P450 and phase II enzymes in liver using transcriptome analyses method and in silico analyses, and we compared the structure of xenobiotic metabolizing enzymes in poultry species to those of mammals.
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Report
(5 results)
Research Products
(116 results)
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[Journal Article] Lead poisoning in children from townships in the vicinity of a lead-zinc mine in Kabwe, Zambia.2015
Author(s)
Yabe J, Nakayama SMM, Ikenaka Y, Yohannes YB, Bortey-Sam N, Oroszlany B, Muzandu K, Choongo K, Kabalo AN, Ntapisha J, Mweene A, Umemura T, Ishizuka M
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Journal Title
Chemosphere
Volume: 119
Pages: 941-947
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Mercury concentrations in primary feathers reflect pollutant exposure in discrete non-breeding grounds used by Short-tailed Shearwaters2015
Author(s)
Watanuki Y, Yamamoto T, Yamashita A, Ishii C, Ikenaka Y, Nakayama SMM, Ishizuka M, Suzuki Y, Niizuma Y, Meathrel CE, Phillips RA
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Journal Title
Journal of Ornithology
Volume: inpress
Pages: inpress
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Identification of inter-specific differences in phase II reactions: Determination of metabolites in the urine of 16 mammalian species exposed to environmental pyrene.2015
Author(s)
Saengtienchai A, Ikenaka Y, Nakayama SMM, Mizukawa H, Kakehi M, Bortey-Sam N, Darwish W, Tsubota T, Terasaki M, Poapolathep A, Ishizuka M
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Journal Title
Environmental Toxicology and Chemistry
Volume: 33
Pages: 2062-2069
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Contamination status and accumulation characteristics of metals and a metalloid in birds on Teuri Island, Hokkaido, Japan2014
Author(s)
Ishii C, Ikenaka Y, Nakayama SMM, Suzuki Y, Watanuki Y, Watanabe Y, Yohannes YB, Mizukawa H, Ishizuka M
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Journal Title
The Japanese Journal of Veterinary Research
Volume: 62
Pages: 143-149
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Radiosensitization of tumor cells through endoplasmic reticulum stress induced by PEGylated nanogel containing gold nanoparticles.2014
Author(s)
Yasui H, Takeuchi R, Nagane M, Meike S, Nakamura Y, Yamamori T, Ikenaka Y, Kon Y, Murotani H, Oishi M, Nagasaki Y, Inanami O
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Journal Title
Cancer Letters
Volume: 347
Pages: 151-158
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Metal distribution in tissues of free-range chickens near a lead-zinc mine in Kabwe, Zambia.2013
Author(s)
Yabe, J., Nakayama, S.M.M., Ikenaka, Y., Muzandu, K., Choongo, K., Mainda, G., Kabeta, N., Ishizuka, M. and Umemura, T
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Journal Title
Environ. Toxicol. Chem.
Volume: 32
Pages: 189-192
Related Report
Peer Reviewed
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[Journal Article] Characterization of Phase-II conjugation reaction of polycyclic aromatic hydrocarbons in fish species: unique pyrene metabolism and species specificity observed in fish species2013
Author(s)
Ikenaka, Y., Oguri, M., Saengtienchai, A., Nakayama, S.M.M., Ijiri, S. and Ishizuka, M. 2013.
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Journal Title
Environ. Toxicol. Pharmacol.
Volume: 36
Pages: 567-578
DOI
Related Report
Peer Reviewed
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[Journal Article] De novo sequence analysis of cytochrome P450 1–3 genes expressed in ostrich liver with highest expression of CYP2G19.2013
Author(s)
Kawai, Y.K., Watanabe, K.P., Ishii, A., Onuma, A., Sawa, H., Ikenaka, Y. and Ishizuka, M.
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Journal Title
Comp. Biochem. Physiol. D
Volume: 8
Pages: 201-208
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular evaluation of a new highly sensitive aryl hydrocarbon receptor in ostriches.2013
Author(s)
Fujisawa, N., Darwish, W.S., Ikenaka, Y., Kim, E., Lee, J., Iwata. H., Nakayama, S.M.M. and Ishizuka, M
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Journal Title
Poult. Sci.
Volume: 92
Pages: 1921-1929.
DOI
Related Report
Peer Reviewed
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[Journal Article] Effects of environmental lead contamination on cattle in a lead/zinc mining area: changes in cattle immune systems on exposure to lead in vivo and in vitro.2012
Author(s)
Ikenaka Y, Nakayama SMM, Muroya T, Yabe J, Konnai S, Darwish WS, Muzandu K, Choongo K, Mainda G, Teraoka H, Umemura T, Ishizuka M.
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Journal Title
Environ Toxicol Chem.
Volume: 31
Pages: 2300-2305
DOI
Related Report
Peer Reviewed
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[Journal Article] TranscriACptional profiling of inflammatory cytokine genes in African buffaloes (Syncerus caffer) infected with Theileria parva.2012
Author(s)
Okagawa T, Konnai S, Mekata H, Githaka N, Suzuki S, Kariuki E, Gakuya F, Kanduma E, Shirai T, Ikebuchi R, Ikenaka Y, Ishizuka M, Murata S, Ohashi K.
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Journal Title
Vet Immunol Immunopathol.
Volume: 148
Pages: 373-379
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular cloning and characterization of Th1 and Th2 cytokines of African buffalo (Syncerus caffer).2012
Author(s)
Suzuki S, Konnai S, Okagawa T, Githaka NW, Kariuki E, Gakuya F, Kanduma E, Shirai T, Ikebuchi R, Ikenaka Y, Ishizuka M, Murata S, Ohashi K
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Journal Title
Int J Immunogenet.
Volume: 39
Pages: 170-182
DOI
Related Report
Peer Reviewed
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[Presentation] Metabolism and biotransformation of organohalogen compounds in the liver microsomes of cats and dogs.2015
Author(s)
Hazuki Mizukawa, Kei Nomiyama, Nozomu, Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Yoshinori Ikenaka, Shouta M.M. Nakayama,Misaki Maehara, Shinsuke Tanabe, Mayumi Ishizuka.
Organizer
SETAC North America 36th Annual Meeting,
Place of Presentation
Salt Palace Convention Center, Salt Lake City, USA
Year and Date
2015-11-01 – 2015-11-05
Related Report
Int'l Joint Research
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[Presentation] Vector-control pesticides disseminating via international waterways to accumulate in free-ranging freshwater and marine fish species2015
Author(s)
Lesa A Thompson, Yoshinori Ikenaka, Johannes J van Vuren, Victor, Wepener, Nico Smit, Wynand Vlok, Yared B. Yohannes, Hazuki Mizukawa, Shouta M.M. Nakayama, Mayumi Ishizuka.
Organizer
Vth International Wildlife Management Congress
Place of Presentation
Sapporo Convention Center, Sapporo, Japan
Year and Date
2015-07-26 – 2015-07-30
Related Report
Int'l Joint Research
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[Presentation] Lead poisoning and source identification in raptors from Japan.2015
Author(s)
Chihiro Ishii, Shouta M.M. Nakayama, Yoshinori Ikenaka, Hazuki Mizukawa, Yared B. Yohannes, Hokuto Nakata, Keisuke Saito, Yukiko Watanabe, Shinsuke Tanabe, Kei Nomiyama, Terutake Hayashi, Yasushi Masuda, Kentaro Q. Sakamoto, Mayumi Ishizuka
Organizer
Vth International Wildlife Management Congress
Place of Presentation
Sapporo Convention Center, Sapporo, Japan
Year and Date
2015-07-26 – 2015-07-30
Related Report
Int'l Joint Research
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[Presentation] 外来化学物質の代謝の種差2015
Author(s)
石塚真由美、中山翔太、水川葉月、池中良徳
Organizer
第31回日本毒性病理学シンポジウム
Place of Presentation
タワーホール船堀、東京
Year and Date
2015-01-29 – 2015-01-29
Related Report
Invited
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[Presentation] Lead pollution in soil, domestic animals and children from townships near a lead-zinc mine in Kabwe, Zambia.2014
Author(s)
Shouta M.M. Nakayama, John Yabe, Yoshinori Ikenaka, Yared B. Yohannes, Balazs Oroszlany, Nesta Bortey-Sam, Kaampwe Muzandu, Kennedy Choongo, Abel Nketani Kabalo, John Ntapisha, Aaron Mweene, Takashi Umemura, Mayumi Ishizuka
Organizer
SOT 53rd Annual Meeting
Place of Presentation
Phoenix Convention Center, Phoenix, Arizona, USA
Year and Date
20140323-20140327
Related Report
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[Presentation] Distribution of toxic metals in free-range chicken, goat and sheep in Ghana and other African countries; and assessment of potential risks to human health through consumption2014
Author(s)
Nesta Bortey-Sam, Shouta M.M. Nakayama, Yoshinori Ikenaka, Yared Beyene Yohannes, Elvis Baidoo, Hazuki Mizukawa, Osei Akoto, Mayumi Ishizuka
Organizer
SETAC North America 35th Annual Meeting
Place of Presentation
Vancouver Convention Centre, Vancouver, Canada
Year and Date
2014-11-09 – 2014-11-13
Related Report
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[Presentation] Contamination status and biotransformation of organohalogen compounds in cats and dogs2014
Author(s)
Hazuki Mizukawa, Kei Nomiyama, Susumu Nakatsu, Akira Kubota, Hisato Iwata, Yoshinori Ikenaka, Shouta M.M. Nakayama, Shinsuke Tanabe, Mayumi Ishizuka
Organizer
12th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
Place of Presentation
Conference Hall of the Kyoto International Foundation, Kyoto, Japan
Year and Date
2014-09-24 – 2014-09-28
Related Report
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[Presentation] Mammalian interspecies differences in xenobiotic phase II reaction2014
Author(s)
Yoshinori Ikenaka, Saengtienchai Aksorn, Mayu Kakehi, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
Organizer
12th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
Place of Presentation
Conference Hall of the Kyoto International Foundation, Kyoto, Japan
Year and Date
2014-09-24 – 2014-09-28
Related Report
Invited
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[Presentation] Species differences of UDP-glucuronosyltransferases (UGTs) in Carnivores2014
Author(s)
Mayu Kakehi, Yoshinori Ikenaka, Shouta MM Nakayama, Hazuki Mizukawa,Kensuke P Watanabe, Kentaro Sakamoto, Akihiko Wada, Kaoru Hattori,Shinsuke Tanabe, Kei Nomiyama, Mayumi Ishizuka
Organizer
12th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
Place of Presentation
Conference Hall of the Kyoto International Foundation, Kyoto, Japan
Year and Date
2014-09-24 – 2014-09-28
Related Report
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[Presentation] Preliminary survey of lead poisoning in raptors from Japan2014
Author(s)
Chihiro Ishii, Shouta M.M. Nakayama, Yoshinori Ikenaka, Hazuki Mizukawa, Yared B. Yohannes, Hokuto Nakata, Keisuke Saito, Yukiko Watanabe, Shinsuke Tanabe, Kei Nomiyama, Terutake Hayashi, Yasushi Masuda, Kentaro Q. Sakamoto, Mayumi Ishizuka
Organizer
6th International Toxicology Symposium in Africa
Place of Presentation
Johannesburg, South Africa
Year and Date
2014-08-29 – 2014-08-29
Related Report
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[Presentation] Investigation of heavy metal contamination and assessment of impact on health in Dandora dumping site, Nairobi2014
Author(s)
Hokuto Nakata, Shouta M.M. Nakayama, Hazuki Mizukawa, Yoshinori Ikenaka, Chihiro Ishii, Yared Beyene Yohannes, John Yabe, Satoru Konnai, Mayumi Ishizuka
Organizer
6th International Toxicology Symposium in Africa
Place of Presentation
Johannesburg, South Africa
Year and Date
2014-08-29 – 2014-08-29
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[Presentation] Human and ecological health risk assessment of pesticides used for vector control: Current situation and problems of DDT use in Africa2014
Author(s)
Yoshinori Ikenaka, Yared Beyene Yohannes, Lesa Thompson, Shouta M.M. Nakayama, Hazuki Mizukawa, Johannes J. van Vuren, Victor Wepener, Nico J. Smit, Volk Wynand, Mayumi Ishizuka
Organizer
6th International Toxicology Symposium in Africa
Place of Presentation
Johannesburg, South Africa
Year and Date
2014-08-29 – 2014-08-29
Related Report
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[Presentation] Effects of international land-based use of vector-control pesticides on a marine ecosystem: organochlorine analysis of fish from Maputo Bay, Mozambique2014
Author(s)
Lesa Thompson, Yoshinori Ikenaka, Yared Beyene Yohannes, Shouta M.M. Nakayama, Hazuki Mizukawa, Johannes J. van Vuren, Victor Wepener, Nico J. Smit, Volk Wynand, Mayumi Ishizuka
Organizer
6th International Toxicology Symposium in Africa
Place of Presentation
Johannesburg, South Africa
Year and Date
2014-08-29 – 2014-08-29
Related Report
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[Presentation] Pb excretion in feces and urine of exposed children from polluted townships in Kabwe, Zambia2014
Author(s)
John Yabe, Shouta M.M. Nakayama, Yoshinori Ikenaka, Yared B Yohannes, Nesta Bortey-Sam, Balazs Oroszlany, Kaampwe Muzandu, Kennedy Choongo, Abel Nketani Kabalo, John Ntapisha, Aaron Mweene, Takashi Umemura, Mayumi Ishizuka
Organizer
6th International Toxicology Symposium in Africa
Place of Presentation
Johannesburg, South Africa
Year and Date
2014-08-29 – 2014-08-29
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[Presentation] Health Risk Assessment of Heavy Metal Residues in Tissues of Cattle and Goats in Benin City, Nigeria2014
Author(s)
Emmanuel Temiotan Ogbomida, Isioma Tongo, Alex Ajeh Enuneku, Martins Oshioriamhe Ainerua, Shouta M.M. Nakayama, Nesta Bortey-Sam, Balazs Oroszlany, Lawrence Ikechukwu Ezemonye, Mayumi Ishizuka
Organizer
6th International Toxicology Symposium in Africa
Place of Presentation
Johannesburg, South Africa
Year and Date
2014-08-29 – 2014-08-29
Related Report
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[Presentation] Antibiotics residues in different poultry meats marketed in Egypt2014
Author(s)
Wageh Sobhy Darwish, Yoshinori Ikenaka, Shouta Nakayama, Mayumi Ishizuka, Eldaly Elsaid, El-Abbasy Mohamed, El-Ghareeb Walid, Tharwat Ahmed, El-Desoky Kamal, Elged Mohamed
Organizer
6th International Toxicology Symposium in Africa
Place of Presentation
Johannesburg, South Africa
Year and Date
2014-08-29 – 2014-08-29
Related Report
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[Presentation] Lead pollution in the children in Kabwe mining area, Republic of Zambia2014
Author(s)
Shouta M.M. Nakayama,John Yabe,Yoshinori Ikenaka,Yared Beyene, Balazs Oroszlany, Nesta Bortey-Sam, Kaampwe Muzandu, Kennedy Choongo, Abel Kabalo, John Ntapisha, Aaron Mweene, Takashi Umemura, Mayumi Ishizuka
Organizer
23rd Symposium on Environmental Chemistry
Place of Presentation
Kyoto University
Year and Date
2014-05-14 – 2014-05-16
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[Presentation] Species difference of warfarin sensitivity among birds can be explained by the two enzymes, cytochrome P450 and vitamin K epoxide reductase.2013
Author(s)
Kensuke P. Watanabe, Yusuke K. Kawai, Minami Kawata, Shouta M.M. Nakayama, Yoshinori Ikenaka, Mayumi Ishizuka,
Organizer
10th International ISSX
Place of Presentation
Westin Harbour Castle Hotel, Toronto, Ontario, Canada
Year and Date
20130929-20131003
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[Presentation] Lead and cadmium pollution in soil and edible tissues of cattle and chickens from townships near a lead-zinc mine in Kabwe, Zambia2013
Author(s)
Shouta M.M. Nakayama, John Yabe, Yoshinori Ikenaka, Kaampwe Muzandu, Kennedy Choongo, Takashi Umemura, Mayumi Ishizuka,
Organizer
5th International Toxicology Symposium in Africa
Place of Presentation
Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Year and Date
20130912-20130913
Related Report
Invited
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