Elucidation of structural basis of glycoconjugate functions and its application for molecular design toward developing drugs targeting the carbohydrate recognition systems

• Project/Area Number: 24249002
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Okazaki Research Facilities, National Institutes of Natural Sciences (2013-2014); Nagoya City University (2012)
• Principal Investigator: KATO Koichi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (20211849)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Takumi 分子科学研究所, 生命・錯体分子科学研究領域, 助教 (60522430) ; YAGI Hirokazu 名古屋市立大学, 薬学研究科(研究院), 講師 (70565423) ; SATOH Tadashi 名古屋市立大学, 薬学研究科(研究院), 准教授 (80532100) ; 矢木 真穂 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), その他部局等, 助教 (40608999)
• Co-Investigator(Renkei-kenkyūsha): YAGI Maho (UTSUMI Maho) 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 特任助教 (40608999)
• Project Period (FY): 2012-10-31 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥39,260,000 (Direct Cost: ¥30,200,000、Indirect Cost: ¥9,060,000); Fiscal Year 2014: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2013: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000); Fiscal Year 2012: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
• Keywords: 構造生物学 / 糖鎖認識 / 細胞内レクチン / 免疫グロブリンG

Outline of Final Research Achievements

In this research project, we have elucidated the glycan-mediated mechanisms of biological functions through structural analyses of molecular complexes and thereby provided the groundwork for developing drugs targeting the carbohydrate recognition systems. In particular, we have revealed molecular mechanisms underlying the selective trafficking and degradation in glycoprotein-fate determination in cells. Furthermore, we have established technical basis for structural analyses of antibodies and thereby obtained solution structure information of these glycoproteins produced in various production vehicles, providing valuable insights into development of antibody drugs.

Research Products

• [Journal Article] 糖鎖構造学研究の新展開 (2015)
  • Author(s): 加藤晃一
  • Journal Title: BIOTOVO Volume: 23 Pages: 2-3
  • Peer Reviewed
• [Journal Article] Emerging structural insights into glycoprotein quality control coupled with N-glycan processing in the endoplasmic reticulum (2015)
  • Author(s): T.Satoh, T.Yamaguchi, and K.Kato
  • Journal Title: Molecules Volume: 20 Issue: 2 Pages: 2475-2491
  • DOI: 10.3390/molecules20022475
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Depicting the Dynamic Conformations of Sugar Chains (2015)
  • Author(s): 山口拓実, 加藤晃一
  • Journal Title: Seibutsu Butsuri Volume: 55 Issue: 2 Pages: 081-083
  • DOI: 10.2142/biophys.55.081
  • NAID: 130005061222
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed / Open Access
• [Journal Article] Backbone 1H, 13C, and 15N resonance assignments of the Fc fragment of human immunoglobulin G glycoprotein (2015)
  • Author(s): H.Yagi, Y.Zhang, M.Yagi-Utsumi, T.Yamaguchi, S.Iida, Y.Yamaguchi, and K.Kato
  • Journal Title: Biomol. NMR Assign. Volume: - Issue: 2 Pages: 383-6
  • DOI: 10.1007/s12104-014-9586-7
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] NMR-based structural validation of therapeutic antibody produced in Nicotiana benthamiana (2015)
  • Author(s): H.Yagi, N.Fukuzawa, Y.Tasaka, K.Matsuo, Y.Zhang, T.Yamaguchi, S.Kondo, S.Nakazawa, N.Hashii, N.Kawasaki, T.Matsumura, and K.Kato
  • Journal Title: Plant Cell Rep. Volume: - Issue: 6 Pages: 959-968
  • DOI: 10.1007/s00299-015-1757-1
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Stable isotope labeling of glycoprotein expressed in silkworms using immunoglobulin G as a test molecule (2015)
  • Author(s): H.Yagi, M.Nakamura, J.Yokoyama, Y.Zhang, T.Yamaguchi, S.Kondo, J.Kobayashi, T.Kato, E.Y.Park, S.Nakazawa, N.Hashii, N.Kawasaki, and K.Kato
  • Journal Title: J. Biomol. NMR Volume: - Issue: 2 Pages: 157-167
  • DOI: 10.1007/s10858-015-9930-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 糖鎖依存的構造異常タンパク質分解に必須な糖鎖刈り込み機構を解明 (2015)
  • Author(s): 蜷川 暁, 加藤晃一, 森 和俊
  • Journal Title: 化学と生物 Volume: -
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Glycan structure and serum half-life of recombinant CTLA4Ig, an immunosuppressive agent, expressed in suspension-cultured rice cells with coexpression of human β1,4-galactosyltransferase and human CTLA4Ig (2015)
  • Author(s): S.H.Kang, H.S.Jung, S.J.Lee1, C.I.Park, S.M.Lim, H.Park, B.S.Kim, K.H.Na, G.J.Han, J.W.Bae, H.J.Park, K.C.Bang, B.T.Park, H.S.Hwang, I.-Soo .Jung, J.I.Kim, D.B.Oh, D.I.Kim, H.Yagi, K.Kato, D.K.Kim, and H.H.Kim
  • Journal Title: Glycoconjugate J. Volume: - Issue: 3-4 Pages: 161-172
  • DOI: 10.1007/s10719-015-9590-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step. (2014)
  • Author(s): Ninagawa S, Okada T, Sumitomo Y, Kamiya Y, Kato K, Horimoto S, Ishikawa T, Takeda S, Sakuma T, Yamamoto T, Mori K
  • Journal Title: J Cell Biol. Volume: 206 Issue: 3 Pages: 347-356
  • DOI: 10.1083/jcb.201404075
  • NAID: 120005468381
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Site-specific classification of N-linked oligosaccharides of the extracellular regions of Fcγ receptor IIIb expressed in baby hamster kidney cells (2014)
  • Author(s): N.Kawasaki, T.Okumoto, Y.Yamaguchi, N.Takahashi, W.H.Fridman, C.Sautes-Fridman, H.Yagi, and K.Kato
  • Journal Title: J. Glycomics Lipidomics Volume: 4 Issue: 02 Pages: 116-116
  • DOI: 10.4172/2153-0637.1000116
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Exploration of conformational spaces of high-mannose-type oligosaccharides by an NMR-validated simulation (2014)
  • Author(s): T.Yamaguchi, Y.Sakae, Y.Zhang, S.Yamamoto, Y.Okamoto, and K.Kato
  • Journal Title: Angew. Chem. Int. Ed. Volume: 53 Issue: 41 Pages: 10941-10944
  • DOI: 10.1002/ange.201406145
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Structural insight into substrate recognition by the endoplasmic reticulum folding-sensor enzyme: crystal structure of third thioredoxin-like domain of UDP-glucose:glycoprotein glucosyltransferase (2014)
  • Author(s): T.Zhu, T.Satoh, and K.Kato
  • Journal Title: Sci. Rep. Volume: 4 Issue: 1 Pages: 7322-7322
  • DOI: 10.1038/srep07322
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Recent advances in glycoprotein production for structural biology: toward tailored design of glycoforms (2014)
  • Author(s): Y.Kamiya, T.Satoh, and K.Kato
  • Journal Title: Curr. Opin. Struct. Biol. Volume: 26 Pages: 44-53
  • DOI: 10.1016/j.sbi.2014.03.008
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 糖鎖構造生物学の最前線 (2014)
  • Author(s): 矢木宏和, 矢木-内海真穂, 加藤晃一
  • Journal Title: ファルマシア Volume: 50 Pages: 746-750
  • NAID: 130005246009
  • Peer Reviewed
• [Journal Article] Structural basis for disparate sugar-binding specificities in the homologous cargo receptors ERGIC-53 and VIP36 (2014)
  • Author(s): Tadashi Satoh, Kousuke Suzuki, Takumi Yamaguchi, and Koichi Kato
  • Journal Title: PLoS ONE Volume: 9 Issue: 2 Pages: e87963-e87963
  • DOI: 10.1371/journal.pone.0087963
  • Peer Reviewed
• [Journal Article] Erol-α and PDIs constitute a hierarchical electron transfer network of endoplasmic reticulum oxidoreductases (2013)
  • Author(s): Kazutaka Araki, Shun-ichiro Iemura, Yukiko Kamiya, David Ron, Koichi Kato, Tohru Natsume, and Kazuhiro Nagata
  • Journal Title: The Journal of Cell Biology Volume: 6 Issue: 6 Pages: 861-874
  • DOI: 10.1083/jcb.201303027
  • Peer Reviewed
• [Journal Article] The unfolded protein response transducer ATF6 represents a novel transmembrane-type endoplasmic reticulum-associated degradation substrate requiring both mannose trimming and SEL1L protein. (2013)
  • Author(s): Horimoto S, Ninagawa S, Okada T, Koba H, Sugimoto T, Kamiya Y, Kato K, Takeda S, Mori K
  • Journal Title: J Biol Chem. Volume: 288 Issue: 44 Pages: 31517-31527
  • DOI: 10.1074/jbc.m113.476010
  • Peer Reviewed
• [Journal Article] AGO61-dependent GlcNAc modification primes the formation of functional glycans on α-dystroglycan. (2013)
  • Author(s): Yagi H, Nakagawa N, Saito T, Kiyonari H, Abe T, Toda T, Wu SW, Khoo KH, Oka S, Kato K.
  • Journal Title: Sci Rep Volume: 3 Issue: 1 Pages: 3288-3288
  • DOI: 10.1038/srep03288
  • NAID: 120005353504
  • Peer Reviewed
• [Journal Article] New NMR tools for characterizing the dynamic conformations and interactions of oligosaccharides (2013)
  • Author(s): Ying Zhang, Takumi Yamaguchi, and Koichi Kato
  • Journal Title: Chem. Lett. Volume: 42 Issue: 12 Pages: 1455-1462
  • DOI: 10.1246/cl.130789
  • NAID: 130004867848
  • Peer Reviewed
• [Journal Article] 神経幹細胞の幹細胞性維持における複合糖質の役割 (2013)
  • Author(s): 矢木宏和，加藤晃一
  • Journal Title: 生化学 Volume: 85 Pages: 1012-1016
  • Peer Reviewed
• [Journal Article] IgG-FcとFc受容体の複合体形成における糖鎖の役割 (2013)
  • Author(s): 矢木宏和，加藤晃一
  • Journal Title: 実験医学 Volume: 31 Pages: 1602-1606
  • Peer Reviewed
• [Journal Article] Application of metabolic ^<13>C labeling in conjunction with high-field nuclear magnetic resonance spectroscopy for comparative conformational analysis of high mannose-type oligosaccharides. (2013)
  • Author(s): Y.Kamiya, K.Yanagi, T.Kitajima, T.Yamaguchi, Y.Chiba, and K.Kato
  • Journal Title: Biomolecules Volume: 3 Issue: 1 Pages: 108-123
  • DOI: 10.3390/biom3010108
  • Peer Reviewed
• [Journal Article] Endoplasmic reticulum lectin XTP3-B inhibits endoplasmic reticulum-associated degradation of a misfolded α1-antitrypsin variant (2013)
  • Author(s): Fujimori T, Kamiya Y, Nagata K, Kato K, Hosokawa N*
  • Journal Title: FEBS J Volume: Vol. 280 Issue: 6 Pages: 1563-1575
  • DOI: 10.1111/febs.12157
  • Peer Reviewed
• [Journal Article] Terminal spin labeling of a high-mannose-type oligosaccharide for quantitative NMR analysis of its dynamic conformation (2013)
  • Author(s): Takumi Yamaguchi
  • Journal Title: Chem. Lett. Volume: 42 Issue: 5 Pages: 544-546
  • DOI: 10.1246/cl.130040
  • NAID: 10031167819
  • Peer Reviewed
• [Presentation] NMR解析に基づく分子シミュレーションによる高マンノース型糖鎖の動的立体構造の解明 (2015)
  • Author(s): 山口拓実, 榮 慶丈, 岡本祐幸, 加藤晃一
  • Organizer: 日本化学会 第95春季年会
  • Place of Presentation: 日本大学(千葉県船橋市）
  • Year and Date: 2015-03-27
• [Presentation] 複合糖質の構造生物学と創薬 (2015)
  • Author(s): 加藤晃一
  • Organizer: 第4回岐阜構造生物学・医学・論理的創薬研究会シンポジウム
  • Place of Presentation: 岐阜薬科大学（岐阜県岐阜市）
  • Year and Date: 2015-03-05
  • Invited
• [Presentation] Conformational dynamics and interactions of oligosaccharides and glycoconjugate (2015)
  • Author(s): Koichi Kato
  • Organizer: 第４回よこはまNMR研究会
  • Place of Presentation: 理化学研究所・横浜研究所交流棟ホール（神奈川県横浜市）
  • Year and Date: 2015-02-04
  • Invited
• [Presentation] Structural basis for sugar trimming reactions by glucosidase II involved glycoprotein quality control system (2015)
  • Author(s): T. Satoh, T. Toshimori, T. Zhu, G. Yan, T. Yamaguchi, and K. Kato
  • Organizer: The 5th Yonsei-IMS Joint Workshop: “Multilateral approaches for investigating biomolecular structures and functions”
  • Place of Presentation: 箱根高原ホテル（神奈川県足柄郡）
  • Year and Date: 2015-01-30 – 2015-02-01
• [Presentation] Structural aspects of glycoprotein folding sensor enzyme (UGGT): A combined X-ray crystallography and small angle X-ray scattering study (2015)
  • Author(s): T. Zhu, T. Satoh, H. Kamikubo, and K. Kato
  • Organizer: The 5th Yonsei-IMS Joint Workshop: “Multilateral approaches for investigating biomolecular structures and functions
  • Place of Presentation: 箱根高原ホテル（神奈川県足柄郡）
  • Year and Date: 2015-01-30 – 2015-02-01
• [Presentation] Elucidation of conformational dynamics of oligosaccharides by NMR spectroscopy and molecular dynamics simulation (2015)
  • Author(s): T. Yamaguchi, Y. Zhang, and K. Kato
  • Organizer: The 5th Yonsei-IMS Joint Workshop: “Multilateral approaches for investigating biomolecular structures and functions
  • Place of Presentation: 箱根高原ホテル（神奈川県足柄郡）
  • Year and Date: 2015-01-30 – 2015-02-01
• [Presentation] Structural views of glycans in physiological and pathological contexts (2015)
  • Author(s): Koichi Kato
  • Organizer: CU-IMS workshop
  • Place of Presentation: Bangkok、Thailand
  • Year and Date: 2015-01-24
  • Invited
• [Presentation] NMRを利用した抗体の高次構造解析 (2015)
  • Author(s): 加藤晃一
  • Organizer: 平成26年度先駆的医薬品・医療機器研究発掘支援事業成果発表会－彩都産学官連携フォーラム2015－
  • Place of Presentation: 千里ライフサイエンスセンター（大阪府豊中市）
  • Year and Date: 2015-01-21
  • Invited
• [Presentation] Structural insight into the ER folding sensor enzyme UDP-glucose: glycoprotein glucosyltransferase (UGGT) (2015)
  • Author(s): T. Zhu, T. Satoh, H. Kamikubo, and K. Kato
  • Organizer: The 3rd International Symposium on Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions
  • Place of Presentation: 合歓の里（三重県志摩市）
  • Year and Date: 2015-01-10 – 2015-01-11
• [Presentation] Elucidation of the molecular basis of oligosaccharide functions by NMR spectroscopy and molecular dynamics simulation (2015)
  • Author(s): T. Yamaguchi, Y. Sakae, M. Yagi-Utsumi, Y. Zhang, S. Yamamoto, Y. Okamoto, and K. Kato
  • Organizer: The 3rd International Symposium on Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions
  • Place of Presentation: 合歓の里（三重県志摩市）
  • Year and Date: 2015-01-10 – 2015-01-11
• [Presentation] Structural basis for recognition of the terminal glucose tag of N-glycans as fate-determinant of glycoproteins in cells (2014)
  • Author(s): T. Satoh, T. Toshimori, K. Suzuki, T. Yamaguchi, G. Yan, T. Zhu and K. Kato
  • Organizer: The 7th Korea-Japan Seminars on Biomolecular Sciences: Experiments and Simulations
  • Place of Presentation: Seoul、Korea
  • Year and Date: 2014-11-26 – 2014-11-27
  • Invited
• [Presentation] EDEM1/2/3 areα1,2-mannosidases essential for endoplasmic reticulum-associated degradation of glycoproteins (2014)
  • Author(s): Satoshi Ninagawa, Tetsuya Okada, Yoshiki Sumitomo, Yukiko Kamiya, Satoshi Horimoto, Tokiro Ishikawa, Shunichi Takeda, Tetsushi Sakuma, Takashi Yamamoto, Koichi Kato and Kazutoshi Mori
  • Organizer: Sarellite Symposium II “Glycans in Neuroscience”
  • Place of Presentation: Honolulu、U.S.A.
  • Year and Date: 2014-11-16
• [Presentation] NMRを利用した抗体の高次構造バリデーション (2014)
  • Author(s): 矢木宏和
  • Organizer: 第5回グライコバイオロジクス研究会
  • Place of Presentation: 臨床研究情報センター（兵庫県神戸市）
  • Year and Date: 2014-11-01
• [Presentation] X線とNMRによる抗体の構造解析 (2014)
  • Author(s): 加藤晃一
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2014-10-18
  • Invited
• [Presentation] タンパク質機能の制御に関わるN型糖鎖構造の多様性と多型性 (2014)
  • Author(s): 加藤晃一
  • Organizer: 第87回 日本生化学会大会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2014-10-17
  • Invited
• [Presentation] 古くて新しい小胞体の糖鎖生物学 (2014)
  • Author(s): 佐藤匡史
  • Organizer: 第87回 日本生化学会大会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2014-10-15
  • Invited
• [Presentation] 小胞体糖タンパク質品質管理に関わるグルコシダーゼIIの基質認識機構 (2014)
  • Author(s): 年森隆泰，佐藤匡史，Gengwei Yan，山口拓実，Tong Zhu，加藤晃一
  • Organizer: 第1回「動的秩序と機能」若手研究会
  • Place of Presentation: ラフォーレ蔵王（宮城県刈田郡）
  • Year and Date: 2014-09-29
• [Presentation] Crystal structure of third thioredoxin-like domain of ER folding sensor glucosyltransferase (2014)
  • Author(s): Tong Zhu, Tadashi Satoh, Koichi Kato
  • Organizer: 第1回「動的秩序と機能」若手研究会
  • Place of Presentation: ラフォーレ蔵王（宮城県刈田郡）
  • Year and Date: 2014-09-28
• [Presentation] 糖タンパク質品質管理システムにおける糖鎖修飾メカニズムの構造基盤Structural basis for the glycan-processing mechanisms in glycoprotein quality control system (2014)
  • Author(s): 佐藤匡史，加藤晃一
  • Organizer: 第52回 日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2014-09-26
  • Invited
• [Presentation] NMR exploration of dynamic conformations and interactions of oligosaccharides and glycoconjugates (2014)
  • Author(s): Koichi Kato
  • Organizer: The 6th Iberoamerican NMR meeting // IV Iberian NMR meeting //VII Reunion Bienal del GERMN
  • Place of Presentation: Alcala de Henares、Spain
  • Year and Date: 2014-09-24
  • Invited
• [Presentation] Structural basis for fate determination and functional regulation of proteins mediated by sugar chains (2014)
  • Author(s): Koichi Kato
  • Organizer: The Cordeliers Research Center Seminar
  • Place of Presentation: Paris、France
  • Year and Date: 2014-09-19
  • Invited
• [Presentation] Structural views of physiological and pathological roles of glycans (2014)
  • Author(s): K. Kato
  • Organizer: Academia Sinica Institutional Lecture
  • Place of Presentation: Taipei 、Taiwan
  • Year and Date: 2014-09-10 – 2014-09-13
  • Invited
• [Presentation] Exploration of conformational dynamics of oligosaccharides by stable isotope- and lanthanide- assisted NMR approaches (2014)
  • Author(s): Takumi Yamaguchi, Ying Zhang, Yukiko Kamiya, Sayoko Yamamoto, and Koichi Kato
  • Organizer: XXVIth International Council on Magnetic Resonance in Biological Systems (ICMRBS)
  • Place of Presentation: Dallas、U.S.A.
  • Year and Date: 2014-08-28
• [Presentation] 小胞体で新生タンパク質に付加されたN型糖鎖からのマンノーストリミングはEDEM2によって開始される (2014)
  • Author(s): 蜷川 暁，岡田徹也，住友嘉樹，神谷由紀子，堀本 賢，石川時郎，武田俊一，佐久間哲史，山本 卓，加藤晃一，森 和俊
  • Organizer: 第33回 日本糖質学会年会
  • Place of Presentation: 名古屋大学(愛知県名古屋市)
  • Year and Date: 2014-08-11
• [Presentation] 小胞体グルコシダーゼIIの基質認識メカニズムの構造基盤 (2014)
  • Author(s): 年森隆泰，佐藤匡史，Gengwei Yan，山口拓実，Tong Zhu，加藤晃一
  • Organizer: 第33回 日本糖質学会年会
  • Place of Presentation: 名古屋大学(愛知県名古屋市)
  • Year and Date: 2014-08-11
• [Presentation] Structural basis for substrate recognition mechanism of ER glucosidase II (2014)
  • Author(s): Tadashi Satoh, Takayasu Toshimori, Takumi Yamaguchi, Zhu Tong, and Koichi Kato
  • Organizer: 23rd Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: Montreal、Canada
  • Year and Date: 2014-08-07
• [Presentation] 糖タンパク質の細胞内プロセッシングに関わるグルコシダーゼⅡの基質認識メカニズム (2014)
  • Author(s): 年森隆泰，佐藤匡史，Gengwei Yan，山口拓実，Tong Zhu，加藤晃一
  • Organizer: 第12回 次世代を担う若手のためのフィジカル・ファーマフォーラム
  • Place of Presentation: 箱根高原ホテル（神奈川県足柄郡）
  • Year and Date: 2014-07-14
• [Presentation] 小胞体品質管理システムに関わるグルコシダーゼIIの基質認識機構Substrate recognition mechanism of glucosidase II involved in ER quality control system (2014)
  • Author(s): 佐藤 匡史，年森 隆泰，山口 拓実，Zhu Tong，加藤 晃一
  • Organizer: 第14回 日本蛋白質科学会年会
  • Place of Presentation: ワークピア横浜（神奈川県横浜市）
  • Year and Date: 2014-06-26
• [Presentation] 揺らめく糖鎖のかたちとはたらき (2014)
  • Author(s): 山口拓実
  • Organizer: 「宇宙・生命・脳・物質・エネルギー」若手研究者による Rising SunⅢ自然科学研究機構若手研究者賞記念講演
  • Place of Presentation: 日本科学未来館（東京都江東区）
  • Year and Date: 2014-06-15
• [Presentation] 小胞体でのN型糖鎖マンノースのトリミングはEDEM2によって開始される (2014)
  • Author(s): 蜷川 暁
  • Organizer: 平成26年度 比較グライコーム研究会
  • Place of Presentation: 名古屋市立大学（愛知県名古屋市）
  • Year and Date: 2014-06-07
• [Presentation] カーゴレセプターERGIC-53/MCFD2複合体による糖タンパク質認識メカニズムの構造基盤 (2014)
  • Author(s): 鈴木康介，佐藤匡史，山口拓実，西尾美穂，神谷由紀子，矢木真穂，加藤晃一
  • Organizer: 第78回 日本生化学会中部支部例会
  • Place of Presentation: 名古屋大学（愛知県名古屋市）
  • Year and Date: 2014-05-24
• [Presentation] 糖タンパク質品質管理に関わるグルコシダーゼIIの基質認識メカニズムの構造基盤 (2014)
  • Author(s): 年森隆泰，佐藤匡史，Genwei Yan，山口拓実，Zhu Tong，加藤晃一
  • Organizer: 第78回 日本生化学会中部支部例会
  • Place of Presentation: 名古屋大学（愛知県名古屋市）
  • Year and Date: 2014-05-24
• [Presentation] Structural views of glycosylation as potential drug target (2014)
  • Author(s): Koichi Kato
  • Organizer: The 4th Asia Pacific Protein Association (APPA) Conference
  • Place of Presentation: Jeju、Korea
  • Year and Date: 2014-05-20
  • Invited
• [Presentation] Structural insights into substrate recognition mechanism of glycoprotein processing enzyme ER glucosidase II (2014)
  • Author(s): Tadashi Satoh, Takayasu Toshimori, Takumi Yamaguchi, Tong Zhu, and Koichi Kato
  • Organizer: The 4th Asia Pacific Protein Association (APPA) Conference
  • Place of Presentation: Jeju、Korea
  • Year and Date: 2014-05-18
• [Presentation] 創薬と生命分子構造学 (2014)
  • Author(s): 加藤晃一
  • Organizer: 名古屋市立大学見学模擬授業
  • Place of Presentation: 名古屋市立大学(愛知県名古屋市）
  • Year and Date: 2014-05-14
  • Invited
• [Presentation] NMR spectroscopic approaches to the conformational dynamics of oligosaccharides by paramagnetic tagging (2012)
  • Author(s): Takumi Yamaguchi
  • Organizer: ISBC2012
  • Place of Presentation: 東京
• [Presentation] NMRを用いた生体分子のダイナミクスの解析
  • Author(s): 加藤晃一
  • Organizer: 大阪市立大学分子ナノ情報解析センターユーザーズミーティング
  • Place of Presentation: 大阪市立大学学術情報総合センター（大阪府）
  • Invited
• [Presentation] 生命分子のダイナミクスと自己組織化
  • Author(s): 加藤晃一
  • Organizer: 国際高等研究所研究プロジェクト 「分子基盤に基づく生体機能への揺らぎとダイナミックネットワークの解明」第１回研究会
  • Place of Presentation: 国際高等研究所（京都府）
  • Invited
• [Presentation] Structural glycobiology for biophysical decoding sweet messages
  • Author(s): Koichi Kato
  • Organizer: 8th Asian Biophysics Association Symposium (ABA Jeju 2013)
  • Place of Presentation: Jeju, Korea
  • Invited
• [Presentation] Paramagnetic NMR approaches for revealing conformational dynamics of oligosaccharides
  • Author(s): Takumi Yamaguchi, Ying Zhang, and Koichi Kato
  • Organizer: 8th Asian Biophysics Association Symposium (ABA Jeju 2013)
  • Place of Presentation: Jeju, Korea
• [Presentation] Recombinant expression of an ER folding sensor UDP-glucose:glycoprotein glucosyltransferase
  • Author(s): Tong Zhu, Maho Yagi-Utsumi, Hirokazu Yagi, Tadashi Satoh, and Koichi Kato
  • Organizer: Korea and Japan joint symposium (Structural and functional studies on biomolecules)
  • Place of Presentation: Jeju, Korea
• [Presentation] 異なる生物基材で発現させた抗体の糖鎖プロファイリング
  • Author(s): 矢木宏和
  • Organizer: 平成25年度 比較グライコーム研究会
  • Place of Presentation: 名古屋市立大学（愛知県）
• [Presentation] NMR analyses of conformational dynamics and interactions of oligosaccharides by employing paramagnetic probes
  • Author(s): Ying Zhang, Takumi Yamaguchi, Sayoko Yamamoto, Yoshinori Uekusa, Maho Yagi-Utsumi, and Koichi Kato
  • Organizer: The 22nd International Glycoconjugate Symposium (GLYCO 22)
  • Place of Presentation: Dalian, China
• [Presentation] ERGIC-53/MCFD2 複合体による糖鎖認識の構造基盤
  • Author(s): 佐藤匡史，鈴木康介，西尾美穂，神谷由紀子，矢木真穂，水島恒裕，加藤晃一
  • Organizer: 第59回 日本薬学会東海支部会
  • Place of Presentation: 名城大学（愛知県）
• [Presentation] 安定同位体標識と常磁性プローブ導入による高マンノース型糖鎖のNMR動的構造解析
  • Author(s): 山口拓実，Zhang Ying，王 進政，喜多島敏彦，千葉靖典，戸谷希一郎，神谷由紀子，加藤晃一
  • Organizer: 第32回日本糖質学会年会
  • Place of Presentation: 大阪国際交流センター（大阪府）
• [Presentation] 積荷受容体ERGIC-53/MCFD2による糖鎖認識の構造基盤
  • Author(s): 佐藤匡史，鈴木康介，西尾美穂，神谷由紀子，矢木真穂，水島恒裕，加藤晃一
  • Organizer: 第32回日本糖質学会年会
  • Place of Presentation: 大阪国際交流センター（大阪府）
• [Presentation] 立体構造からみた糖鎖の不均一性
  • Author(s): 加藤晃一
  • Organizer: 第32回日本糖質学会年会
  • Place of Presentation: 大阪国際交流センター（大阪府）
  • Invited
• [Presentation] NMRを用いた糖鎖のコンフォメーション揺らぎとクラスター特性の解析
  • Author(s): 山口拓実，加藤晃一
  • Organizer: 国際高等研研究プロジェクト「分子基盤に基づく生体機能ネットワークと ダイナミクスの解明」第2回研究会
  • Place of Presentation: 国際高等研究所（京都府）
  • Invited
• [Presentation] NMRを用いた生命分子のダイナミクス解析
  • Author(s): 加藤晃一
  • Organizer: 第2回 立命館大学BKC生体分子ネットワークセミナー
  • Place of Presentation: 立命館大学（滋賀県）
  • Invited
• [Presentation] Atomic anatomy of antibody as glycoprotein
  • Author(s): Koichi Kato
  • Organizer: World Biopharma Week China Focus 2013
  • Place of Presentation: Shanghai, China
  • Invited
• [Presentation] Paramagnetism-assisted NMR for atomic description of dynamic oligosaccharides
  • Author(s): Ying Zhang, Takumi Yamaguchi, Sayoko Yamamoto, and Koichi Kato
  • Organizer: 糖鎖科学中部拠点 第11回若手の力フォーラム
  • Place of Presentation: 名古屋市立大学（愛知県）
• [Presentation] Bacterial expression and EM analysis of UDP-glucose glycosyltransferase from a thermophilic fungus
  • Author(s): Tong Zhu, Kazuyoshi Murata, Takahasu Toshimori, Maho Yagi-Utsumi, Tadashi Sato, and Koichi Kato
  • Organizer: 糖鎖科学中部拠点 第11回若手の力フォーラム
  • Place of Presentation: 名古屋市立大学（愛知県）
• [Presentation] バイオ医薬品の構造をみる
  • Author(s): 加藤晃一
  • Organizer: 第22回日本バイオイメージング学会学術集会
  • Place of Presentation: 東京大学薬学部講堂（東京都文京区）
  • Invited
• [Presentation] 糖鎖が担う タンパク質社会の秩序維持
  • Author(s): 加藤晃一
  • Organizer: 市民公開講座・第99回分子科学フォーラム
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県）
  • Invited
• [Presentation] ランタニドイオンを活用した常磁性NMR法による糖鎖の動的構造解析
  • Author(s): 山口拓実，Zhang Ying，王 進政，戸谷希一郎，榮 慶丈，岡本祐幸，神谷由紀子，加藤 晃一
  • Organizer: 第7回バイオ関連化学シンポジウム
  • Place of Presentation: 名古屋大学(愛知県）
• [Presentation] 常磁性効果を活用した糖鎖の立体構造解析－NMRと分子動力学計算によるコンフォメーション空間の探査－
  • Author(s): 山口拓実
  • Organizer: 日本分光学会NMR分光部会
  • Place of Presentation: 名古屋大学理学研究科付属構造生物学研究センター（愛知県）
  • Invited
• [Presentation] 多次元HPLC法およびNMR法による糖鎖解析技術
  • Author(s): 加藤晃一
  • Organizer: 抗体バイオ分析セミナー－抗体/バイオ医薬品における各種分析手法および解析手法－
  • Place of Presentation: ゆうぽうと(東京都品川区）
  • Invited
• [Presentation] NMR characterization of dynamic conformational ensembles of oligosaccharides and intermolecular interactions in glycolipid clusters
  • Author(s): Koichi Kato
  • Organizer: 5th Asia-Pacific NMR Symposium in conjunction with ANZMAG 2013
  • Place of Presentation: Brisbane, Australia
  • Invited
• [Presentation] 糖鎖の機能解明を目指したNMRアプローチ
  • Author(s): 加藤晃一
  • Organizer: 第52回NMR討論会
  • Place of Presentation: 石川県立音楽堂（石川県）
  • Invited
• [Presentation] EM tomography analysis of ER folding sensor enzyme UDP-glucose: glycoprotein glucosyltransferase
  • Author(s): Tong Zhu, Kazuyoshi Murata, Takayasu Toshimori, Takumi Yamaguchi, Maho Yagi-Utsumi, Tadashi Satoh, and Koichi Kato
  • Organizer: 平成25年度 生理学研究所研究会「電子顕微鏡機能イメージングの医学・生物学への応用」
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県）
• [Presentation] 糖鎖を見る：分子模型からムービーへ
  • Author(s): 加藤晃一
  • Organizer: 平成25年度 生理学研究所研究会「構造の多様性に立脚した糖鎖機能の解明に向けて」
  • Place of Presentation: 生理学研究所（愛知県）
  • Invited
• [Presentation] Overall structure of ER folding sensor UDP-glucose: glycoprotein glucosyltransferase revealed by EM tomography
  • Author(s): Tong Zhu, Kazuyoshi Murata, Takayasu Toshimori, Takumi Yamaguchi, Maho Yagi-Utsumi, Tadashi Satoh, and Koichi Kato
  • Organizer: 山田研究会・統合バイオサイエンスシンポジウム
  • Place of Presentation: 伊良湖ビューホテル（愛知県）
• [Presentation] タンパク質社会の秩序維持における糖鎖の役割
  • Author(s): 加藤晃一
  • Organizer: お茶の水女子大学 糖鎖科学教育研究センター 第6回 公開シンポジウム
  • Place of Presentation: お茶の水女子大学(東京都文京区）
  • Invited
• [Presentation] Paramagnetism-assisted NMR evaluation of molecular dynamics simulations for conformational characterization of oligosaccharides
  • Author(s): Takumi Yamaguchi, Ying Zhang, Yukiko Kamiya, Yoshitake Sakae, Yuko Okamoto, and Koichi Kato
  • Organizer: 6th Japan-Korea Seminars on Biomolecular Science : Experiments and Simulation
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県）
• [Presentation] Position selective 13C labeling of multi-antennary oligosaccharides for NMR analysis
  • Author(s): Panuwat Padungros, Takumi Yamaguchi, and Koichi Kato
  • Organizer: SOKENDAI Asian Winter School "Innovations and Challenges in Molecular Science: From Basics to Cutting-edge Researches”
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県）
• [Presentation] 核磁気共鳴法による糖鎖の3次元構造ダイナミクスの解明
  • Author(s): 山口拓実
  • Organizer: 第 2 回 NINS Colloquium
  • Place of Presentation: ヤマハリゾートつま恋（静岡県）
• [Presentation] Structural biology of glycoconjugates: sugar chains as therapeutic targets
  • Author(s): Koichi Kato
  • Organizer: Yonsei-IMS Seminars on Biomolecular Sciences : Protein Structure and Diseases
  • Place of Presentation: Busan, Korea
  • Invited
• [Presentation] Structural basis for disparate sugar-binding specificities in the homologous cargo receptors ERGIC-53 and VIP36
  • Author(s): Tadashi Satoh, Kosuke Suzuki, Takumi Yamaguchi, and Koichi Kato
  • Organizer: Yonsei-IMS Seminars on Biomolecular Sciences : Protein Structure and Diseases
  • Place of Presentation: Busan, Korea
  • Invited
• [Presentation] Elucidation of the conformational dynamics of oligosaccharides by using paramagnetism-assisted NMR spectroscopy
  • Author(s): Ying Zhang, Takumi Yamaguchi, Sayoko Yamamoto, and Koichi Kato
  • Organizer: Yonsei-IMS Seminars on Biomolecular Sciences : Protein Structure and Diseases
  • Place of Presentation: Busan, Korea
• [Presentation] The structural biology study on ER folding sensor UDP-glucose:glycoprotein glucosyltransferase (UGGT)
  • Author(s): Tong Zhu, Kazuyoshi Murata, Takayasu Toshimori, Takumi Yamaguchi, Maho Yagi-Utsumi, Tadashi Satoh, and Koichi Kato
  • Organizer: Yonsei-IMS Seminars on Biomolecular Sciences : Protein Structure and Diseases
  • Place of Presentation: Busan, Korea
• [Presentation] Conformational dynamics of oligosaccharides characterized by paramagnetism-assisted NMR spectroscopy in conjunction with molecular dynamics simulation
  • Author(s): Koichi Kato
  • Organizer: The 10th International Symposium on Biochemical roles of Eukaryotic Cell Surface Macromolecules
  • Place of Presentation: Kolkata, India
  • Invited
• [Presentation] NMR approaches for elucidating the functional roles of carbohydrate chains
  • Author(s): Koichi Kato
  • Organizer: 20th Symposium of National Magnetic Resonance Society (NMRS-2014)
  • Place of Presentation: Assam, India
  • Invited
• [Presentation] 生命分子の揺らぎと秩序形成
  • Author(s): 加藤晃一
  • Organizer: 日本学術振興会 分子系の複合電子機能第181委員会
  • Place of Presentation: 国際高等研究所（京都府）
  • Invited
• [Presentation] 超高磁場NMR分光法を中心としたタンパク質の高次構造・相互作用解析
  • Author(s): 加藤晃一
  • Organizer: 第2回 ISITナノ・バイオフォーラム
  • Place of Presentation: 福岡市産学連携交流センター(福岡県）
  • Invited
• [Presentation] 小胞体品質管理に関わるグルコシダーゼIIの基質認識機構の構造基盤
  • Author(s): 年森隆泰，佐藤匡史，Zhu Tong，加藤晃一
  • Organizer: 2013年度 生物物理中部支部会
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県）
• [Presentation] Structural-architecture of the ER glycoprotein folding sensor UDP-glucose:glycoprotein glucosyltransferase (UGGT)
  • Author(s): Tong Zhu, Tadashi Satoh, Kazuyoshi Murata, Hironari Kamikubo, Takayasu Toshimori, Takumi Yamaguchi, Maho Yagi-Utsumi, and Koichi Kato
  • Organizer: 2013年度 生物物理中部支部会
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県）
• [Presentation] NMRを応用した糖鎖の動的構造解析
  • Author(s): 加藤晃一
  • Organizer: よこはまNMR構造生物学研究会 第49回ワークショップ
  • Place of Presentation: 理化学研究所横浜研究所交流棟ホール(神奈川県）
  • Invited
• [Presentation] 小胞体糖タンパク質フォールディング装置を構成するタンパク質群の構造生物学研究
  • Author(s): 佐藤匡史，年森隆泰，Tong Zhu，村田和義，山口拓実，矢木真穂，加藤晃一
  • Organizer: 日本薬学会134年会
  • Place of Presentation: 熊本大学（熊本県）
• [Presentation] Elucidation of the conformational dynamics of oligosaccharides by paramagnetism-assisted NMR approach
  • Author(s): Ying Zhang, Takumi Yamaguchi, Sayoko Yamamoto, Yoshitake Sakae, Yuko Okamoto, and Koichi Kato
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学（愛知県）
• [Presentation] 常磁性効果を応用した糖鎖の動的立体構造解析
  • Author(s): 山口拓実
  • Organizer: 第51回NMR討論会
  • Place of Presentation: 名古屋
• [Presentation] Conformational dynamics and interactions of glycoconjugates of therapeutic interest
  • Author(s): Koichi Kato
  • Organizer: Commemorative Symposium on the 20th Anniversary of the Mizutani Foundation for Glycoscience
  • Place of Presentation: 東京
  • Invited
• [Presentation] 複合糖質の構造生物学： 創薬標的としての糖鎖
  • Author(s): 加藤晃一
  • Organizer: 第40回構造活性相関シンポジウム
  • Place of Presentation: 岡崎
  • Invited
• [Presentation] HPLCによる糖鎖の構造解析およびバリデーションの方法
  • Author(s): 矢木宏和
  • Organizer: 技術情報協会セミナー「バイオ/抗体医薬品の各種分析手法とバリデーション」
  • Place of Presentation: 東京
  • Invited
• [Presentation] High resolution analysis of protein glycosylation
  • Author(s): Koichi Kato
  • Organizer: CASSS CMC Strategy Forum Japan 2012
  • Place of Presentation: 東京
  • Invited
• [Presentation] 糖タンパク質の運命を決める細胞内レクチンの糖鎖認識メカニズムを“見る”
  • Author(s): 佐藤匡史
  • Organizer: 糖鎖科学中部拠点「糖鎖科学講義」
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] NMR characterization of conformational fluctuations of oligosaccharides and glycoconjugates
  • Author(s): Koichi Kato
  • Organizer: The 6th International Symposium, Molecular Science of Fluctuations toward Biological Functions
  • Place of Presentation: 京都
  • Invited
• [Presentation] Conformations, dynamics, and interactions of sugar chains as studied by NMR spectroscopy
  • Author(s): Yoshinori Uekusa
  • Organizer: The 6th International Symposium, Molecular Science of Fluctuations toward Biological Functions
  • Place of Presentation: 京都
• [Presentation] Characterization of conformational dynamics of branched oligosaccharides by using paramagnetic NMR techniques
  • Author(s): Ying Zhang
  • Organizer: 9th SOKENDAI Student Seminar (Life Science Retreat 2012)
  • Place of Presentation: 静岡
• [Presentation] 複合糖質の立体構造・ダイナミクス・相互作用
  • Author(s): 加藤晃一
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] Comparative conformational analysis of high-mannose-type oligosaccharides using high-field NMR spectroscopy combined with 13C-labeling technique
  • Author(s): Kotaro Yanagi
  • Organizer: Sokendai Asian Winter School "Frontiers in Photo-Molecular Science"
  • Place of Presentation: 岡崎
• [Presentation] Structural views of carbohydrate-protein interaction systems as potential therapeutic targets
  • Author(s): Koichi Kato
  • Organizer: Kasetsart University Special Seminar
  • Place of Presentation: Bangkok, Thailand
  • Invited
• [Presentation] Paramagnetic lanthanide-tagging for NMR conformational characterization of glycolipids
  • Author(s): Takumi Yamaguchi
  • Organizer: Pure and Applied Chemistry International Conference 2013 (PACCON 2013)
  • Place of Presentation: Chon Buri, Thailand
• [Presentation] Structural views of carbohydrate-protein interaction systems as potential therapeutic targets
  • Author(s): Koichi Kato
  • Organizer: Pure and Applied Chemistry International Conference 2013 (PACCON 2013)
  • Place of Presentation: Chon Buri, Thailand
  • Invited
• [Presentation] NMR approaches to the molecular basis of oligosaccharide functions
  • Author(s): Takumi Yamaguchi
  • Organizer: 2013 Asian Core Winter School
  • Place of Presentation: Busan, Korea
  • Invited
• [Presentation] 生命分子複合体の動態解明への実験的アプローチ
  • Author(s): 加藤晃一
  • Organizer: 京都大学原子炉実験所 粒子線基礎物性研究特別講演会
  • Place of Presentation: 熊取
  • Invited
• [Presentation] Paramagnetic NMR approaches for characterization of conformational dynamics and interactions of oligosaccharides
  • Author(s): Zhang Ying
  • Organizer: 日本生物物理学会中部支部講演会
  • Place of Presentation: 名古屋
• [Presentation] Conformational dynamics and interactions of oligosaccharides in physiological and pathological contexts
  • Author(s): Koichi Kato
  • Organizer: Fifth Korea-Japan Seminars on Biomolecular Sciences: Experiments and Simulations
  • Place of Presentation: Korea
  • Invited
• [Presentation] Molecular and structural basis for N-glycan-dependent determination of glycoprotein fates in cells
  • Author(s): Koichi Kato
  • Organizer: Glycobiology Gordon Research Conference
  • Place of Presentation: Ventura, USA
  • Invited
• [Presentation] NMRとSANSによるタンパク質の動的構造解析
  • Author(s): 加藤晃一
  • Organizer: 中性子連携研究会
  • Place of Presentation: 品川
  • Invited
• [Presentation] NMRによる糖鎖クラスターのダイナミクスと相互作用の解析
  • Author(s): 加藤晃一
  • Organizer: 糖鎖集合状態の変化による幹細胞近接場制御についての討論会
  • Place of Presentation: 岡崎
• [Presentation] NMRを用いた生体分子のダイナミクスの解析
  • Author(s): 加藤晃一
  • Organizer: 大阪市立大学分子ナノ情報解析センターユーザーズミーティング
  • Place of Presentation: 大阪
  • Invited
• [Presentation] 生命分子のダイナミクスと自己組織化
  • Author(s): 加藤晃一
  • Organizer: 国際高等研究所研究プロジェクト 「分子基盤に基づく生体機能への揺らぎとダイナミックネットワークの解明」第１回研究会
  • Place of Presentation: 奈良
• [Book] Advances in Neurobiology (2014)
  • Author(s): Y.Yamaguchi, T.Yamaguchi, and K.Kato
  • Total Pages: 584
  • Publisher: Springer (New York)
• [Book] Advances in Experimental Medicine and Biology (2014)
  • Author(s): Y.Zhang, T.Yamaguchi, T.Satoh, M.Yagi-Utsumi, Y.Kamiya, Y.Sakae, Y.Okamoto, and K.Kato
  • Total Pages: 411
  • Publisher: Springer (Switzerland)
• [Remarks] 構造異常糖タンパク質の分解に必須な糖鎖刈り込み機構を解明
  • URL: http://www.kyoto-u.ac.jp/ja/research/research_results/2014/140902_1.html
• [Remarks] 水中で絶え間なく揺らいでいる糖鎖の立体構造を描き出す
  • URL: https://www.ims.ac.jp/news/2014/09/05_2994.html