Development of a method for simultaneous recordings of single channel dynamics and currents
| Project/Area Number |
24249013
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
IWAMOTO Masayuki 福井大学, 医学部, 助教 (40452122)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥47,710,000 (Direct Cost: ¥36,700,000、Indirect Cost: ¥11,010,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥37,180,000 (Direct Cost: ¥28,600,000、Indirect Cost: ¥8,580,000)
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| Keywords | 蛋白質 / 1分子動態計測 / 1分子計測(SMD) / 生理学 / １分子計測（SMD） / X線 |
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| Outline of Final Research Achievements |
Prior to realizing simultaneous recordings of single-molecular dynamics and currents we tried to increase time-resolution of the dynamics measurementin this project. We intorduced a X-ray focusing mirror and a spectrum measurement system to the sychrotorn facility(SPring8). The time resolutions of the dynamics measurements became to be in a sub-millisecond level, which is equivalent to that of single channel current measurement. And the X-ray radiation damages of the ion channel proteins were decreased by using the spectrum measurement system. These advances of the method will lead to the development of simultaneous measurement system in near future.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] ATR-FTIR Spectroscopy Revealed the Different Vibrational Modes of the Selectivity Filter Interacting with K+ and Na+ in the Open and Collapsed Conformations of the KcsA Potassium Channel2012
Author(s)
Furutani, Y., Shimizu, H., Asai, Y., Fukuda, T., Oiki, S. and Kandori, H
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Journal Title
J. Phys. Chem. Lett
Volume: 3
Issue: 24
Pages: 3806-3810
DOI
Related Report
Peer Reviewed
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