Biogeneis and function of germ cell specific small RNA
| Project/Area Number |
24249018
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
Nakano Toru 大阪大学, 生命機能研究科, 教授 (00172370)
|
|---|
| Project Period (FY) |
2012-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥45,760,000 (Direct Cost: ¥35,200,000、Indirect Cost: ¥10,560,000)
Fiscal Year 2014: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2013: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2012: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
|
|---|
| Keywords | 非コードRNA / 遺伝子発現 / DNA メチル化 / 生殖細胞 / 小分子RNA / 遺伝子発現制御 / アポトーシス / レトロチランスポゾン / MVH / 精子形成 / ミトコンドリア / GPAT2 / piRNA |
|---|
| Outline of Final Research Achievements |
piRNAs are germ cell specific non-coding small RNAs, which play crucial roles on gene silencing of retrotransposons in embryonic male germ cells. We carried out the analyses on the molecular mechanisms of piRNA biogenesis and the functional properties of the piRNAs, and obtained the following results. First, GPAT2 (glycerol-3-phosophate acyltransferase 2), a mitochondria outer-membrane protein with the enzymatic activity of lipid metabolism, was essential for the production of piRNAs. Second, piRNA other than retrotransposon-related ones was successfully produced in transgenic mouse model. Third, the artificially introduced non-retrotransposon piRNAs gave rise to DNA methylation and subsequent gene silencing in the male germ cells. These results provide new insights in the characteristics of piRNAs.
|
Report
(4 results)
Research Products
(16 results)
-
[Journal Article] Induction of DNA Methylation by Artificial piRNA Production in Male Germ Cells2015
Author(s)
Itou D, Shiromoto Y, Shin-Ya Y, Ishii C, Nishimura T, Ogonuki N, Ogura A, Hasuwa H, Fujihara Y, Kuramochi-Miyagawa S, Nakano T.
-
Journal Title
Current biology
Volume: 25
Issue: 7
Pages: 901-906
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
[Journal Article] GPAT2, a Mitochondrial Outer Membrane Protein in piRNA Biogenesis in Germline Stem Cells2013
Author(s)
Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Katanaya A, Nishimura K, Ohtaka M, Nakanishi M, Nakamura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojima-Kita K, Itou D, Kimura T, Nakano T
-
Journal Title
RNA
Volume: 19
Issue: 6
Pages: 803-810
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Targeted gene silencing in mouse germ cells by insertion of a homologous DNA into a piRNA generating locus2013
Author(s)
Yamamoto Y,Watanabe T,Hoki Y, Shirane K, Li Y, Ichiiyanagi K, Kuramochi-Miyagawa S, Toyoda A, Fujiyama A, Oginuma M, Suzuki H, Sado T, Nakano T, Sasaki H
-
Journal Title
Genome Res
Volume: 23
Issue: 2
Pages: 292-299
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] PGC7 binds histone H3K9me2 to protect against conversion of 5MeC to 5HmC in early embryos2012
Author(s)
Nakamura, T., Liu, Y.U., Nakashima, H., Umehara, H., Inoue, K., Matoba, S., Tachibana, M., Ogura, A., Shinkai, Y., Nakano, T
-
Journal Title
Nature
Volume: 486
Issue: 7403
Pages: 415-419
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
