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Biogeneis and function of germ cell specific small RNA

Research Project

Project/Area Number 24249018
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionOsaka University

Principal Investigator

Nakano Toru  大阪大学, 生命機能研究科, 教授 (00172370)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥45,760,000 (Direct Cost: ¥35,200,000、Indirect Cost: ¥10,560,000)
Fiscal Year 2014: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2013: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2012: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Keywords非コードRNA / 遺伝子発現 / DNA メチル化 / 生殖細胞 / 小分子RNA / 遺伝子発現制御 / アポトーシス / レトロチランスポゾン / MVH / 精子形成 / ミトコンドリア / GPAT2 / piRNA
Outline of Final Research Achievements

piRNAs are germ cell specific non-coding small RNAs, which play crucial roles on gene silencing of retrotransposons in embryonic male germ cells. We carried out the analyses on the molecular mechanisms of piRNA biogenesis and the functional properties of the piRNAs, and obtained the following results. First, GPAT2 (glycerol-3-phosophate acyltransferase 2), a mitochondria outer-membrane protein with the enzymatic activity of lipid metabolism, was essential for the production of piRNAs. Second, piRNA other than retrotransposon-related ones was successfully produced in transgenic mouse model. Third, the artificially introduced non-retrotransposon piRNAs gave rise to DNA methylation and subsequent gene silencing in the male germ cells. These results provide new insights in the characteristics of piRNAs.

Report

(4 results)
  • 2015 Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (16 results)

All 2015 2013 2012

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 8 results)

  • [Journal Article] Induction of DNA Methylation by Artificial piRNA Production in Male Germ Cells2015

    • Author(s)
      Itou D, Shiromoto Y, Shin-Ya Y, Ishii C, Nishimura T, Ogonuki N, Ogura A, Hasuwa H, Fujihara Y, Kuramochi-Miyagawa S, Nakano T.
    • Journal Title

      Current biology

      Volume: 25 Issue: 7 Pages: 901-906

    • DOI

      10.1016/j.cub.2015.01.060

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comprehensive DNA Methylation Analysis of Retrotransposons in Male Germ Cells2015

    • Author(s)
      Nagamori I, Kobayashi H, Shiromoto Y, Nishimura T, Kuramochi-Miyagawa S, Kono T, Nakano T.
    • Journal Title

      Cell Rep.

      Volume: 12 Issue: 10 Pages: 1541-1547

    • DOI

      10.1016/j.celrep.2015.07.060

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] GPAT2, a Mitochondrial Outer Membrane Protein in piRNA Biogenesis in Germline Stem Cells2013

    • Author(s)
      Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Katanaya A, Nishimura K, Ohtaka M, Nakanishi M, Nakamura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojima-Kita K, Itou D, Kimura T, Nakano T
    • Journal Title

      RNA

      Volume: 19 Issue: 6 Pages: 803-810

    • DOI

      10.1261/rna.038521.113

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Dppa3 on DNA methylation dynamics during primordial germ cell development2013

    • Author(s)
      Nakashima H, Kimura T, Kaga Y. Nakatani T, Seki Y, Nakamura T, Nakano T
    • Journal Title

      Biol Rep

      Volume: 88 Issue: 5 Pages: 1-9

    • DOI

      10.1095/biolreprod.112.105932

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Targeted gene silencing in mouse germ cells by insertion of a homologous DNA into a piRNA generating locus2013

    • Author(s)
      Yamamoto Y,Watanabe T,Hoki Y, Shirane K, Li Y, Ichiiyanagi K, Kuramochi-Miyagawa S, Toyoda A, Fujiyama A, Oginuma M, Suzuki H, Sado T, Nakano T, Sasaki H
    • Journal Title

      Genome Res

      Volume: 23 Issue: 2 Pages: 292-299

    • DOI

      10.1101/gr.137224.112

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A forward genetic screen to study mammalian RNA interference - essential role of RNase IIIa domain of Dicer1 in 3' strand cleavage of dsRNA in vivo2012

    • Author(s)
      Ohishi K, Nakano T
    • Journal Title

      FEBS J.

      Volume: 279 Issue: 5 Pages: 832-43

    • DOI

      10.1111/j.1742-4658.2012.08474.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Essential role of DPPA3 for chromatin condensation in mouse oocytogenesis2012

    • Author(s)
      Liu YJ, Nakamura T, Nakano T
    • Journal Title

      Biol Rep

      Volume: 86 Issue: 2 Pages: 1-8

    • DOI

      10.1095/biolreprod.111.095018

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PGC7 binds histone H3K9me2 to protect against conversion of 5MeC to 5HmC in early embryos2012

    • Author(s)
      Nakamura, T., Liu, Y.U., Nakashima, H., Umehara, H., Inoue, K., Matoba, S., Tachibana, M., Ogura, A., Shinkai, Y., Nakano, T
    • Journal Title

      Nature

      Volume: 486 Issue: 7403 Pages: 415-419

    • DOI

      10.1038/nature11093

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] DNA Methylation and Gene Silencing of Retrotransposons by piRNA in Embryonic Male Germ Cells2015

    • Author(s)
      Toru Nakano
    • Organizer
      Conférences Jacques Monod DNA Methylation and Demethylation
    • Place of Presentation
      ブリトニー、フランス
    • Year and Date
      2015-11-17
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] DNAメチル化と発生・発がん2013

    • Author(s)
      仲野 徹
    • Organizer
      日本病理学会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Artificial Induction of Germ Cell Specific Gene Silencing through piRNA pathway2013

    • Author(s)
      仲野 徹
    • Organizer
      Recent Advances in Stem Cell Biology
    • Place of Presentation
      九州大学 博多
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Artificial Induction of Germ Cell Specific Gene Silencing through piRNA pathway2013

    • Author(s)
      仲野 徹
    • Organizer
      8th Annual Conference of Asia Epigenome Alliance
    • Place of Presentation
      台北(台湾)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Artificial Induction of Germ Cell Specific Gene Silencing through piRNA pathway2013

    • Author(s)
      仲野 徹
    • Organizer
      Small RNAs to Stem Cells & Epigenetic Reprogramming Asia-2013
    • Place of Presentation
      東京大学 東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] DNA Methylation in Cell Differentiation and Transformation2012

    • Author(s)
      仲野 徹
    • Organizer
      Shanghai International Conference of Epigenetics in Development and Disease
    • Place of Presentation
      上海・中国
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] piRNA and Spermatogenesis2012

    • Author(s)
      仲野 徹
    • Organizer
      2nd SKLRB Symposia
    • Place of Presentation
      北京・中国
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] piRNA産生システムの解析2012

    • Author(s)
      仲野 徹
    • Organizer
      22nd HCS 4th JARI Joint International Symposium
    • Place of Presentation
      広島
    • Related Report
      2012 Annual Research Report
    • Invited

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Published: 2013-05-15   Modified: 2019-07-29  

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