| Budget Amount *help |
¥43,290,000 (Direct Cost: ¥33,300,000、Indirect Cost: ¥9,990,000)
Fiscal Year 2014: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2013: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2012: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
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| Outline of Final Research Achievements |
We have established a sensitive, easy, rapid, and quantitative method by incorporating an immunomagnetic method and a genetic method so-called the LAMP method to detect pathogenic (tdh- and/or trh-positive) strains of Vibrio parahaemolyticus. We demonstrated the dried-type LAMP reagent made possible the maintenance and transportation of the reagent and eliminated the need for gel electrophoresis to judge the result in the workshops held in seven developing countries in Asia and South America. We found loss of hemolytic activity of the trh gene product due to frequent base substitutions in this gene of environmental strains, and thus we are adjusting the LAMP primers for this gene to improve this detection method.
Regarding the enterohemorrhagic Escherichia coli, we developed a similar qualitative detection method for the strains belonging to 12 important O serotypes in the world from beef at once.
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