Development of easy, sensitive, and quantitative detection methods for enteric pathogens of word importance from food
| Project/Area Number |
24249038
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGUCHI Yoshitsugu 石川県立大学, 生物資源環境学部, 准教授 (70378967)
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| Co-Investigator(Renkei-kenkyūsha) |
SETO Kazuko 大阪府立公衆衛生研究所, 主任研究員 (70211323)
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| Project Period (FY) |
2012-10-31 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥43,290,000 (Direct Cost: ¥33,300,000、Indirect Cost: ¥9,990,000)
Fiscal Year 2014: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2013: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2012: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
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| Keywords | 腸炎ビブリオ / 腸管出血性大腸菌 / 免疫磁気ビーズ法 / LAMP / IMS / 食中毒細菌 / コレラ菌 / 国際研究者交流 タイ / O157 / 国際研究者交流 / タイ / マレーシア |
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| Outline of Final Research Achievements |
We have established a sensitive, easy, rapid, and quantitative method by incorporating an immunomagnetic method and a genetic method so-called the LAMP method to detect pathogenic (tdh- and/or trh-positive) strains of Vibrio parahaemolyticus. We demonstrated the dried-type LAMP reagent made possible the maintenance and transportation of the reagent and eliminated the need for gel electrophoresis to judge the result in the workshops held in seven developing countries in Asia and South America. We found loss of hemolytic activity of the trh gene product due to frequent base substitutions in this gene of environmental strains, and thus we are adjusting the LAMP primers for this gene to improve this detection method.
Regarding the enterohemorrhagic Escherichia coli, we developed a similar qualitative detection method for the strains belonging to 12 important O serotypes in the world from beef at once.
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Report
(4 results)
Research Products
(77 results)
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[Journal Article] Detection and quantification of pathogenic Vibrio parahaemolyticus in shellfish by using multiplex PCR and loop-mediated isothermal amplification assay2015
Author(s)
Malcolm , T. T. H., Y. K. Cheah, C. W. J. W. M. Radzi, F. A. Kasim, H. K. Kantilal, T. Y. H. John, J. Martinez-Urtaza, Y. Nakaguchi, M. Nishibuchi, R. Son
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Journal Title
Food Control.
Volume: 47
Pages: 664-671
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Effect of detergents as antibacterial agents on biofilm of antibiotics-resistant Vibrio parahaemolyticus isolate2014
Author(s)
Elexson, N., L. Afsah-Hejri, Y. Rukayadi, P. Soopna, H.Y. Lee, T.C. Tuan Zainazor, M. Nor Ainy, Y. Nakaguchi, Nishibuchi Mitsuaki, R. Son
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Journal Title
Food control
Volume: 35
Issue: 1
Pages: 378-385
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Biofilm assessment of Vibrio parahaemolyticus from seafood using Random Amplified Polymorphism DNA-PCR.2014
Author(s)
Elexson, N., Yaya, R., Nor, A. M., Kantilal, H. K., Ubong, A., Yoshitsugu, N., Nishibuchi, M. Son, R.
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Journal Title
Int. Food Res. J
Volume: 21(1)
Pages: 59-65
Related Report
Peer Reviewed
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[Journal Article] Effect of detergents as antibacterial agents on biofilm of antibiotics-resistant Vibrio parahaemolyticus isolate.2014
Author(s)
Elexson, N., L. Afsah-Hejri, Y. Rukayadi, P. Soopna, H.Y. Lee, T.C. Tuan Zainazor, M. Nor Ainy, Y. Nakaguchi, Mitsuaki Nishibuchi, R. Son.
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Journal Title
Food Control
Volume: 50
Pages: 378-385
Related Report
Peer Reviewed
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[Journal Article] Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk2013
Author(s)
Lye, Y. L., Afsah-Hejri, L., Chang, W. S., Loo, Y. Y., Puspanadan, S., Kuan, C. H., Goh, S. G., Shahril, N., Rukayadi, Y., Khatib, A., John, Y.H.T., Nishibuchi, M., Nakaguchi, Y. Son, R
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Journal Title
Int. Food Res. J.
Volume: 20(2)
Pages: 1001-1005
Related Report
Peer Reviewed
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[Journal Article] Quantification of Escherichia coli O157:H7 in organic vegetables and chickens2013
Author(s)
Chang, W.S., Afsah-Hejri, L., Rukayadi, Y., Khatib, A., Lye, Y. L., Loo, Y. Y., Mohd Shahril, N., Puspanadan, S., Kuan, C.H., Goh, S.G., John, Y.H.T., Nakaguchi, Y., Nishibuchi, M. Son, R.
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Journal Title
Int. Food Res. J.
Volume: 20(2)
Pages: 1023-1029
Related Report
Peer Reviewed
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[Journal Article] Characterization of extended-spectrum β -lactamases (ESBLs) producers in Klebsiella pneumoniae by genotypic and phenotypic method.2013
Author(s)
Puspanadan, S., Afsah-Hejri, L., John, Y.H.T., Rukayadi, Y., Loo, Y.Y, Nillian, E., Kuan, C.H., Goh, S.G., Chang, W.S., Lye, Y.L., Mohd Shahril, N., Nakaguchi, Y., Nishibuchi, M., Son, R.
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Journal Title
Int. Food Res. J.
Volume: 20(3)
Pages: 1479-1483
Related Report
Peer Reviewed
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[Journal Article] Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia2013
Author(s)
Loo, Y. Y, Puspanadan, S., Goh, S. G., Kuan, C. H., Chang, W. S., Lye, Y. L., John, Y. H. T, Rukayadi, Y., Yoshitsugu, N. Nishibuchi, M. Son, R.
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Journal Title
Int. Food Res. J
Volume: 20(6)
Pages: 3313-3317
Related Report
Peer Reviewed
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[Presentation] Relative pathogenic significance of the thermostable direct hemolysn (TDH, TDH-related hemolysin, and type 3 secretion system of Vibrio parahaemolyticus as evaluated by their gene sequence and penetration through the human intestinal epitherial cell Caco-2 monolayer.2014
Author(s)
Okuda, J., C. Suezawa, M. Yasuda, C. Kunikata, O. R. Maldonado, Y. Nakaguchi, and M. Nishibuchi
Organizer
48thUS-Japan Cooperative Medical Sciences Program Conference on Cholera and Other Bacterial Enteric Infections.
Place of Presentation
International Centre for Diarrheal Diseases, Bangladesh.
Related Report
Invited
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[Presentation] TDH-related Hemolysin, and Type 3 Secretion System of Vibrio parahaemolyticus as Evaluated by Their Gene Sequence and Penetration through the Human Intestinal Epithelial Cell Caco-2 Monolayer2014
Author(s)
Jun Okuda, Chigusa Suezawa, Masashi Yasuda, Chinami Kunikata, Oscar Roberto Escalante Maldonado, Yoshitugu Nakaguchi, Mitsuaki Nishibuchi
Organizer
第４８回日米医学研究会コレラ・細菌性腸管感染症専門部会合同会議
Place of Presentation
Icddr, b, Dhaka, Bangladesh
Related Report
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