Clarification of physiological developmental pathway of myeloid lineage cells
| Project/Area Number |
24249057
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥45,630,000 (Direct Cost: ¥35,100,000、Indirect Cost: ¥10,530,000)
Fiscal Year 2014: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2013: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2012: ¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
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| Keywords | 系列決定 / フェイトマッピング / エピジェネティクス / T細胞系列 / B細胞系列 / 造血 / 分化経路 / 前駆細胞 |
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| Outline of Final Research Achievements |
This project aimed to clarify physiological developmental pathway of myeloid lineage cells by using “fate mapping” method. To this aim, we genetically marked myelo-lymphoid (Flt3 positive) progenitors by using Flt3-Cre mice. However, in this setting, all hematopoietic cells were marked. We also used inducible system (Flt3-ERCre mice), but in this setting, marking ratio was too low to get conclusive results. Therefore we have to say that we failed to achieve the original plan during the period. On the other hand, in another line of study related to this subject, we chased the fate of hematopietic clone in human hematopoiesis. The results provided very important findings that individual hematopoietic stem cells produce only limited lineage cells (Stem cells, 2013). To explain this phenomenon, we proposed “mosaic commitment-inducing microenvironment model”, and simulation results based on this model agreed well with clinical data.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Loss of mTOR complex 1 induces developmental blockage in early T-lymphopoiesis and eradicates T-cell acute lymphoblastic leukemia cells.2013
Author(s)
Hoshii T, Kasada A, Hatakeyama T, Ohtani M, Tadokoro Y, Naka K, Ikenoue T, Ikawa T, Kawamoto H, Fehling HJ, Araki K, Yamamura K, Matsuda S, Hirao A
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 111
Issue: 10
Pages: 3805-3810
DOI
Related Report
Peer Reviewed
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[Journal Article] MicroRNA-126-mediated control of cell fate in B-cell myeloid progenitors as a potential alternative to transcriptional factors.2013
Author(s)
Okuyama K, Ikawa T, Gentner B, Hozumi K, Harnprasopwat R, Lu J, Yamashita R, Ha D, Toyoshima T, Chanda B, Kawamata T, Yokoyama K, Wang S, Ando K, Lodish HF, Tojo A, Kawamoto H, Kotani A
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 110
Issue: 33
Pages: 13410-13415
DOI
Related Report
Peer Reviewed
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[Journal Article] Development and function of invariant natural killer T cells producing t(h)2- and t(h)17-cytokines.2012
Author(s)
Watarai H, Sekine-Kondo E, Shigeura T, Motomura Y, Yasuda T, Satoh R, Yoshida H, Kubo M, Kawamoto H, Koseki H, Taniguchi M.
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Journal Title
PLOS Biology
Volume: 10
Issue: 7
Pages: e1001255-e1001255
DOI
Related Report
Peer Reviewed
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[Journal Article] Process for immune defect and chromosomal translocation during early thymocyte development lacking ATM2012
Author(s)
Isoda T, Takagi M, Piao J, Nakagama S,Sato M, Masuda K, Ikawa T, Azuma M, Morio T, Kawamoto H, Mizutani S
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Journal Title
Blood
Volume: 120(4)
Issue: 4
Pages: 789-9
DOI
Related Report
Peer Reviewed
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