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Control of citrus greening disease using new microorganisms in Asian agricultural enviroment

Research Project

Project/Area Number 24255018
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Boundary agriculture
Research InstitutionNishinippon Junior College (2013-2015)
Kyushu University (2012)

Principal Investigator

SHIMIZU SUSUMU  西日本短期大学, その他部局等, 特任教授 (20187454)

Co-Investigator(Kenkyū-buntansha) TAKAGI MASAMI  九州大学, 農学研究院, 教授 (20175425)
UECHI NAMI  独立行政法人農業・食品産業技術総合研究機構, 果樹試験場・品種育成・病害虫領域, 研究員 (40507597)
ICHINOSE KATUYA  独立行政法人農業・食品産業技術総合研究機構, 国際農林水産産業研究センター・沖縄支所, 農林水産技官 (70355642)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥44,070,000 (Direct Cost: ¥33,900,000、Indirect Cost: ¥10,170,000)
Fiscal Year 2015: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2014: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2013: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Keywordsカンキツグリーニング病 / 媒介虫 / ミカンキジラミ / 寄生菌 / 拮抗微生物 / 防除 / 新規微生物
Outline of Final Research Achievements

The Asian citrus psyllid, Diaphorina citri Kuwayama, (Hemiptera: Psyllidae) was discovered in Okinawa in 1998 and apparently arrived without its natural enemies. Control of D. citri is important because this phloem-feeding pest vectors Candidatus Liberibacter asiaticus (Ca. L. asiaticus), a phloem-residing α-proteobacterium that causes citrus greening disease or Huanglongbing. This research contributes the first steps towards evaluating entomopathogenic fungi for its potential use in an IPM program to suppress D. citri populations. Fungal pathogens that killed adult D. citri in Thailand, Vietnam, Okinawa and Amami-oshima were identified and characterized. Isaria javanica from Okinawa has a high virulence against D. citri and was effective on control of D. citri in laboratory and field applications.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (16 results)

All 2016 2015 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (11 results) (of which Invited: 3 results)

  • [Journal Article] Effective molecular detection of Diaphorina citri Kuwayama (Hemiptera: Liviidae) in bulk insect samples from sticky traps.2016

    • Author(s)
      藤原和樹,上地奈美,清水優子,土田聡,井上広光,山口卓宏,岩波徹,藤川貴史
    • Journal Title

      Journal of Applied Entomology

      Volume: 8 Issue: 1-2 Pages: 1-6

    • DOI

      10.1111/jen.12315

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Metarhizium 属糸状菌の最近の研究動向 -分類と新機能を中心としてー2014

    • Author(s)
      清水 進
    • Journal Title

      蚕糸・昆虫バイオテック

      Volume: 83 Pages: 153-158

    • NAID

      130004705725

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ミカンキジラミDiaphorina citri成虫(半翅目:キジラミ科)の定位に対するカラー粘着トラlップおよび反射資材の影響2014

    • Author(s)
      上地奈美・宮田伸一・岩波徹
    • Journal Title

      日本応用動物昆虫学会誌

      Volume: 58 Pages: 119-125

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of the germination rates of Metarhizium spp. conidia from Japan at high and low temperatures2013

    • Author(s)
      Nishi, O., Iiyama, K., Yasunaga-Aoki, C. and Shimizu, S.
    • Journal Title

      Letters in Applied Microbiology

      Volume: 57 Pages: 554-560

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asian citrus greening and assessment of current techniques for reducing disease occurrences.2013

    • Author(s)
      Iwanami, T., Tuan, DH, Ichinose, K.
    • Journal Title

      Journal of Agricultural Science and Applications

      Volume: 2 Pages: 13-21

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 天敵微生物をつかった害虫防除2015

    • Author(s)
      清水進
    • Organizer
      門司植物防疫所植物防疫官研修
    • Place of Presentation
      門司植物防疫所
    • Year and Date
      2015-10-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 天敵微生物による害虫防除2015

    • Author(s)
      清水進
    • Organizer
      樹木医実践技術講座
    • Place of Presentation
      西日本短期大学
    • Year and Date
      2015-08-29
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Pathogenicity of two entomopathogenic fungi isolated on Diaphorina citri and their potential of reducible effect on the vector population.2015

    • Author(s)
      Shimizu, S., Chau, N. M., Hoa, N. V., Huynh, N. V., Duong,T., thuy, Q., Uechi, N., Hayashi, T., and Ichinose, K.
    • Organizer
      4th International Research Conference On Huanglongbing (HLB)
    • Place of Presentation
      Orlando, Florida USA
    • Year and Date
      2015-02-09 – 2015-02-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 天敵糸状菌を用いたミカンキジラミ防除の可能性2015

    • Author(s)
      清水 進
    • Organizer
      カンキツグリーニング病研究会
    • Place of Presentation
      つくば国際会議室
    • Year and Date
      2015-02-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] ミカンキジラミ成虫の定位に対するカラー粘着トラップおよび反射資材の影響2014

    • Author(s)
      上地奈美・宮田伸一・岩波徹
    • Organizer
      第58回日本応用動物昆虫学会大会
    • Place of Presentation
      高知大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] カンキツグリーニング病と媒介虫ミカンキジラミの防除に関する成果と展望2014

    • Author(s)
      上地奈美・井上広光
    • Organizer
      第58回日本応用動物昆虫学会大会
    • Place of Presentation
      高知大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 天敵糸状菌による害虫防除の現状と課題2013

    • Author(s)
      清水 進
    • Organizer
      九州病害虫防除推進協議会創立43周年記念講和会
    • Place of Presentation
      福岡ガーデンパレス
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Comparative virulence analysis of entomopathogenic fungus Metarhizium spp. in different ambient temperature conditions2013

    • Author(s)
      Oumi Nishi, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu
    • Organizer
      International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia
    • Place of Presentation
      宮崎大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Metarhizium属糸状菌の研究動向とSMZ-2000の性状2013

    • Author(s)
      清水 進
    • Organizer
      第57回日本応用動物昆虫学会
    • Place of Presentation
      日本大学生物資源科学部 神奈川
    • Related Report
      2012 Annual Research Report
  • [Presentation] Metarhizium 糸状菌の昆虫と生息環境に対する適応進化2012

    • Author(s)
      西 大海・飯山和弘・青木智佐・清水 進
    • Organizer
      第10回昆虫病理研究会シンポジウム
    • Place of Presentation
      帯広畜産大学 北海道
    • Related Report
      2012 Annual Research Report
  • [Presentation] Host-dependent lineage diversification of Scarabaeidae-specific pathogen Metarhizium majus.2012

    • Author(s)
      Oumi Nishi, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu
    • Organizer
      International Congress on Invertebrate Pathology and Microbial Control and 45th Annual Meeting of the Society for Invertebrate Pathology
    • Place of Presentation
      Buenos Aires, Argentina
    • Related Report
      2012 Annual Research Report

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Published: 2013-05-15   Modified: 2019-07-29  

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