Development of statistical methodology for imaging mass spectrometry

• Project/Area Number: 24300110
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Statistical science
• Research Institution: Teikyo University (2014); Japanese Foundation for Cancer Research (2012-2013)
• Principal Investigator: MATSUURA Masaaki 帝京大学, 大学院公衆衛生学研究科, 教授 (40173794)
• Co-Investigator(Kenkyū-buntansha): EGUCHI Shinto 統計数理研究所, 数理推論研究系, 教授 (10168776) ; SETOU Mitsutoshi 浜松医科大学, 医学部, 教授 (20302664) ; USHIJIMA Masaru 公益財団法人がん研究会, ゲノムセンター, 研究員 (60328565) ; MIYATA Satoshi 東北大学, 医学系研究科, 准教授 (60360343) ; YAO Ikuko 浜松医科大学, メディカルフォトニクス研究センター, 准教授 (60399681) ; HOSHIKAWA Yutaka 公益財団法人がん研究会, がん研究所, 研究員 (80280626) ; WAKUI Masatoshi 慶應義塾大学, 医学部, 講師 (90240465) ; 森 誠一 公益財団法人がん研究会, がん研究会 がんゲノム研究部, 主任研究員 (10334814)
• Co-Investigator(Renkei-kenkyūsha): KAJIHARA Shigeki 島津製作所, 基盤技術研究所, 主任研究員 (10395246)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: 質量分析 / 統計 / 画像 / 画像処理 / 顕微質量装置 / イメージ / 質量顕微鏡法

Outline of Final Research Achievements

In imaging mass spectrometry, we can obtain lazar captured mass spectrometry data from cancer tissues or normal cells using a microscope. These data generate imaging data in which each spot has a spectrum with many peaks for small molecules. We have developed statistical algorithm and software which can detect important similar peaks including of a cancer site, for example.

Research Products

• [Journal Article] Identification of nitrated tyrosine residues of protein kinase G-Ia by mass spectrometry. (2014)
  • Author(s): Lu J, Yao I, Shimojo M, Katano T, Uchida H, Setou M, Ito S
  • Journal Title: Analytical and bioanalytical chemistry Volume: 406 Issue: 5 Pages: 1387-1396
  • DOI: 10.1007/s00216-013-7535-4
  • Peer Reviewed
• [Journal Article] Development of imaging mass spectrometry (2012)
  • Author(s): Saito Y, Waki M, Hameed S, Hayasaka T, Setou M.
  • Journal Title: Biol Pharm Bull. Volume: 35 Pages: 1417-1424
  • NAID: 130001872090
  • Peer Reviewed
• [Journal Article] Visualization of acetylcholine distribution in central nervous system tissue sections by tandem imaging mass spectrometry. (2012)
  • Author(s): Sugiura Y, Zaima N, Setou M, Ito S, Yao I.
  • Journal Title: Anal Bioanal Chem. Volume: 403 Issue: 7 Pages: 1851-1861
  • DOI: 10.1007/s00216-012-5988-5
  • Peer Reviewed
• [Journal Article] Development of Imaging Mass Spectrometry (2012)
  • Author(s): Yamazaki H, Chijiwa T, Inoue Y, Abe Y, Suemizu H, Kawai K, Wakui M, Furukawa D, Mukai M, Kuwao S, Saegusa M, Nakamura M.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 35 Issue: 9 Pages: 1417-1424
  • DOI: 10.1248/bpb.b212007
  • NAID: 130001872090
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Presentation] Similarity index for image patterns from imaging mass spectrometry. (2015)
  • Author(s): Matsuura M, Ushijima M, Kajihara S
  • Organizer: Pacific Symposium on Biocomputing 2015
  • Place of Presentation: Hawaii (USA)
  • Year and Date: 2015-01-04 – 2015-01-08
• [Presentation] Classification of breast cancer subtypes using lasso clustering methods (2013)
  • Author(s): Ushijima M, Eguchi S, Komori S, Miki Y, Matsuura M
  • Organizer: 9th Japan America Joint International Mass Spectrometry Conference
  • Place of Presentation: Hawaii, America
  • Year and Date: 2013-02-23
• [Presentation] 質量分析イメージングによる脳情報の可視化-医学への貢献を目指して (2013)
  • Author(s): 矢尾育子
  • Organizer: 第38回医用マススペクトル学会
  • Place of Presentation: 神戸
• [Presentation] Optimization of conditions for acetylcholine detection in the nerve tissue sections by imaging mass spectrometry (2012)
  • Author(s): Yao I, Sugiura Y, Zaima N, Setou M, Ito S
  • Organizer: 9th Joint Conference of the American Association for Cancer Research and the Japanese Cancer Association
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2012-09-17
• [Presentation] Analysis of imaging pattern for Imaging mass spectrometry data (2012)
  • Author(s): Ushijima M, Eguchi S, Kajihara S, Matsuura M.
  • Organizer: International Biometrics Conference 2012
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-08-30
• [Presentation] Visualization of acetylcholine distribution in the brain by imaging mass spectrometry (2012)
  • Author(s): Yao I, Sugiura Y, Zaima N, Setou M, Ito S
  • Organizer: 60th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2012-05-21
• [Book] Imaging Mass Spectrometry (IMS) for Biological Application Mass Spectrometry Handbook, pp41-84 (2012)
  • Author(s): Sugiura Y, Yao I, Setou M
  • Total Pages: 44
  • Publisher: Wiley