Spatiotemporal control mechanisms of neural circuit formation

• Project/Area Number: 24300116
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Chiba University
• Principal Investigator: SAITO Tetsuichiro 千葉大学, 医学(系)研究科(研究院), 教授 (00202078)
• Co-Investigator(Kenkyū-buntansha): MUROYAMA Yuko 千葉大学, 大学院医学研究院, 特任講師 (20422248) ; SATO Tatsuya 千葉大学, 大学院医学研究院, 助教 (00568222)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 遺伝子 / 細胞・組織 / 神経科学 / 脳・神経 / 発生・分化 / 脊髄 / 神経細胞 / 電気穿孔法 / テトラサイクリン

Outline of Final Research Achievements

Neural circuit formation requires fine spatiotemporal control of each step, such as neuronal differentiation and axonal projection. We have developed a new method in which gene expression is turned on in an aimed portion of the mouse nervous system in vivo. Mice that have a defect in a restricted part of neural circuits and a specific behavioral disorder have been efficiently obtained.

Research Products

• [Journal Article] In utero electroporation of the mouse embryo (2015)
  • Author(s): Tetsuichiro Saito
  • Journal Title: Neuromethods Volume: 102 Pages: 1-20
  • DOI: 10.1007/978-1-4939-2459-2_1
  • ISBN: 9781493924585, 9781493924592
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Exo utero electroporation of the mouse embryo (2015)
  • Author(s): Tetsuichiro Saito
  • Journal Title: Neuromethods Volume: 102 Pages: 21-31
  • DOI: 10.1007/978-1-4939-2459-2_2
  • ISBN: 9781493924585, 9781493924592
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Electroporation of dissociated hippocampal neurons (2015)
  • Author(s): Atsushi Baba, Tetsuichiro Saito
  • Journal Title: Neuromethods Volume: 102 Pages: 169-178
  • DOI: 10.1007/978-1-4939-2459-2_13
  • ISBN: 9781493924585, 9781493924592
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Control of gene expression in neurons by the use of in vivo electroporation and the tetracycline system (2015)
  • Author(s): Tatsuya Sato, Yuko Muroyama, Tetsuichiro Saito
  • Journal Title: Neuromethods Volume: 102 Pages: 187-195
  • DOI: 10.1007/978-1-4939-2459-2_15
  • ISBN: 9781493924585, 9781493924592
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Control of gene expression in neurons by the use of in vivo electroporation and the tetracycline system (2014)
  • Author(s): Tatsuya Sato, Yuko Muroyama, Tetsuichiro Saito
  • Journal Title: NeuroMethods Volume: 印刷中
• [Journal Article] Inducible gene expression in postmitotic neurons by an in vivo electroporation-based tetracycline system (2013)
  • Author(s): Sato, T., Muroyama, Y. and Saito, T
  • Journal Title: Journal of Neuroscience Methods Volume: 214 Issue: 2 Pages: 170-176
  • DOI: 10.1016/j.jneumeth.2013.01.014
  • Peer Reviewed
• [Presentation] Postmitotic dorsal spinal cord neurons are transfated into commissural neurons by induced misexpression of Barh1 (2014)
  • Author(s): Tatsuya Sato, Yuko Muroyama, Tetsuichiro Saito
  • Organizer: 北米神経科学会2014年会
  • Place of Presentation: Washington, DC, U.S.A.
  • Year and Date: 2014-11-18
• [Presentation] ニューロンにおける新たな遺伝子発現誘導系の開発 (2013)
  • Author(s): 佐藤達也
  • Organizer: 第8回Chiba Neuroresearch Meeting
  • Place of Presentation: 京葉銀行文化プラザ(千葉市)
  • Year and Date: 2013-01-19
• [Presentation] 大脳新皮質の幹細胞の初期プログラム (2012)
  • Author(s): 斎藤哲一郎
  • Organizer: 包括脳夏のワークショップ
  • Place of Presentation: 仙台国際センター(仙台市)
  • Year and Date: 2012-07-25
• [Presentation] Specification of commissural neurons and formation of neural circuits
  • Author(s): Tetsuichiro Saito
  • Organizer: The First Symposium of Leading Graduate School at Chiba University Immune Regulation and Innovative Therapeutics
  • Place of Presentation: ステーションコンファレンス東京（東京）
  • Invited
• [Presentation] 新皮質神経幹細胞の初期プログラム
  • Author(s): 斎藤 哲一郎
  • Organizer: 新学術領域 山森・門松・宮田合同シンポジウム「糖鎖・細胞動態・皮質構築」
  • Place of Presentation: 名古屋国際会議場（名古屋）
  • Invited
• [Presentation] Tightly controlled induction of gene expression is achieved in postmitotic neurons by the combination of the tetracycline system and in vivo electroporation
  • Author(s): Tatsuya Sato, Yuko Muroyama, Tetsuichiro Saito
  • Organizer: Neuro2013サテライトシンポジウム"Molecular & Cellular Mechanisms of Brain Development & Evolution"
  • Place of Presentation: 京都府立医科大学（京都）
• [Presentation] Gene induction to postmitotic neurons by the use of in vivo electroporation and the tetracycline system
  • Author(s): Tatsuya Sato, Yuko Muroyama, Tetsuichiro Saito
  • Organizer: Neurogenesis 2013 in Matsushima
  • Place of Presentation: ホテル松島大観荘（松島）
• [Presentation] テトラサイクリン遺伝子発現誘導システムと電気穿孔法を組み合わせることにより分化した神経細胞において遺伝子発現を誘導できる
  • Author(s): 佐藤 達也、室山 優子、斎藤 哲一郎
  • Organizer: 第36回分子生物学会年会
  • Place of Presentation: 神戸国際会議場（神戸）
• [Remarks] 千葉大学 大学院医学研究院 発生再生医学
  • URL: http://www.m.chiba-u.ac.jp/class/dev/index.html
• [Remarks] 千葉大学 大学院医学研究院 発生再生医学
  • URL: http://www.m.chiba-u.ac.jp/class/dev/index.html
• [Remarks]
  • URL: http://www/,/cjoba-i/ac.jp/class/dev/index.html