| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we examined the dynamics of endogenous AMPA-type glutamate receptors at the intact synapses in acute hippocampal slices using photochemical inactivation by using photoreactive AMPA receptor antagonist ANQX. Postsynaptic AMPA receptors are less mobile at the resting condition, and the synaptic delivery from intracellular reserve pools were accererated during induction of long-term potentiation. We also adopted photochemical inactivation in hippocampal slices obtained from knock out mice of PSD95, one of the major proteins of postsynaptic density. In PSD95 kockout mice, synaptic delivery were accererated during the resting condition than wild type mice. These results suggested that PSD95 serves as a receptor slot to limit the mobility of AMPA receptors during the resting condition, and may upregulated after the induction of long-term potentiation to increase the synaptic efficacy.
|
