| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
|
|---|
| Outline of Final Research Achievements |
We have obtained the following new findings during the support period. (1) At the calyx of Held synapse in the auditory brainstem, we found that Intersectin, an endocytotic protein, is involved in synaptic vesicle replenishment (Sakaba et al., 2013, PNAS), suggesting tight coupling between exo- and endocytosis. In addition, using KI mice, we found that Munc13-1 is the downstream target of calmodulin and regulates synaptic vesicle replenishment and recovery from short-term synaptic depression (Lipstein et al., 2013, Neuron). (2) At the Purkinje cell output synapse, we have performed direct presynaptic recordings and found that action potential attenuation specifically at the presynaptic terminal, but not at the axon, determines short-term synaptic depression (Kawaguchi and Sakaba, 2015, Neuron). Unlike glutamatergic synapses, synaptic vesicle dynamics may not be a limiting factor at inhibitory synapses. This issue needs future investigation.
|
