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Elucidation of comprehensive MHC information in common marmoset for biomedical studies

Research Project

Project/Area Number 24300155
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionTokai University

Principal Investigator

SHIINA Takashi  東海大学, 医学部, 准教授 (00317744)

Co-Investigator(Kenkyū-buntansha) KAMETANI Yoshie  東海大学, 医学部, 准教授 (50338787)
SUZUKI Ryuji  独立行政法人国立病院機構, 臨床研究センター, 室長 (70373470)
Co-Investigator(Renkei-kenkyūsha) SASAKI Erika  実験動物中央研究所, 応用発生学研究部, 部長 (70390739)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Keywordsマーモセット / MHC / 遺伝子多型 / 遺伝子発現 / 遺伝子機能 / 免疫 / ジェノタイピング / TCR / MHC / 多型 / 機能 / 再生医療
Outline of Final Research Achievements

In this study, we characterized major histocompatibility complex (MHC) and MHC-related genes and molecules in common marmosets (Callithrix jacchus) by performing polymorphism, expression and functional analyses of such as polymorphism analysis and next generation sequencing based genotyping using 85 related and unrelated animals, specification of immune cells that are driven by MHC expression using flow cytometry, removal method for micro-chimeric cells using GFP transgenic marmoset, development of monoclonal antibodies against MHC class I molecules, development of analysis tool for typing of T cell receptor genes and quantitation of cytokines, and preparing specimens from standard organs and tissues for future immunological studies.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (18 results)

All 2015 2014 2013 2012

All Journal Article (11 results) (of which Peer Reviewed: 10 results,  Open Access: 1 results) Presentation (7 results)

  • [Journal Article] Formation of infectious dengue virus-antibody immune complex in vivo in marmosets (Callithrix jacchus) after passive transfer of anti-dengue virus monoclonal antibodies and infection with dengue virus.2015

    • Author(s)
      Moi ML, Ami Y, Shirai K, Lim CK, Suzaki Y, Saito Y, Kitaura K, Saijo M, Suzuki R, Kurane I, Takasaki T.
    • Journal Title

      Am J Trop Med Hyg.

      Volume: 92 Issue: 2 Pages: 370-376

    • DOI

      10.4269/ajtmh.14-0455

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NKG2D functions as an activating receptor on natural killer cells in common marmoset (callithrix jacchus).2014

    • Author(s)
      Watanabe M, Kudo Y, Kawano M, Nakayama M, Nakamura K, Kameda M, Ebara M, Sato T, Nakamura M, Omine K, Kametani Y, Suzuki R, Ogasawara K.
    • Journal Title

      Int Immunol.

      Volume: 26 Issue: 11 Pages: 597-606

    • DOI

      10.1093/intimm/dxu053

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genomic sequence analysis of the major histocompatibility complex (MHC) class I G/F segment in common marmoset (Callithrix jacchus)2014

    • Author(s)
      Kono A, Brameier M, Roos C, Suzuki S, Shigenari A, Kametani Y, Kitaura K, Matsutani T, Suzuki R, Inoko H, Walter L, Shiina T
    • Journal Title

      J Immunol.

      Volume: 192 Issue: 7 Pages: 3239-3246

    • DOI

      10.4049/jimmunol.1302745

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Defect of tropomyosin-related kinase B isotype expression in ovarian clear cell adenocarcinoma.2014

    • Author(s)
      Goto Y et al.
    • Journal Title

      BioScience Trends

      Volume: 未定

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] エストロゲンと胎盤形成;新たな非ヒト霊長類コモンマーモセットを用いたヒト妊娠免疫モデルの提案2014

    • Author(s)
      亀谷美恵 他
    • Journal Title

      比較内分泌学

      Volume: 40 Pages: 11-13

    • NAID

      130004509413

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Immune-Related Gene Expression Profile in Laboratory Common Marmosets Assessed by an Accurate Quantitative Real-Time PCR Using Selected Reference Genes.2013

    • Author(s)
      Fujii Y
    • Journal Title

      Pros ONE

      Volume: 8 Issue: 2 Pages: e56296-e56296

    • DOI

      10.1371/journal.pone.0056296

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 非ヒト霊長類コモンマーモセット免疫系の重度免疫不全マウスへの移植による再構築2013

    • Author(s)
      亀谷美恵 他
    • Journal Title

      J.Germfree life and Gnotobiol.

      Volume: 43 Pages: 113-116

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Double expression of CD34 and CD117 on bone marrow progenitors is a hallmark of the development of functional mast cell of Calithrix Jucchus (common marmoset).2012

    • Author(s)
      Nunomura S
    • Journal Title

      Int. Immunol

      Volume: 24 Issue: 9 Pages: 593-603

    • DOI

      10.1093/intimm/dxs070

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new method for quantitative analysis of the T cell receptor V region repertoires in healthy common marmosets by microplate hybridization assay.2012

    • Author(s)
      Kitaura K
    • Journal Title

      J Immunol Methods

      Volume: 364 Issue: 1-2 Pages: 81-91

    • DOI

      10.1016/j.jim.2012.07.012

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Study of cynomolgus monkey (Macaca fascicularis) Mhc DRB gene polymorphism in four populations2012

    • Author(s)
      Blancher A, et al.
    • Journal Title

      Immunogenetics

      Volume: 64(8) Issue: 8 Pages: 605-614

    • DOI

      10.1007/s00251-012-0613-5

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nomenclature report on the major histocompatibility complex genes and alleles of Great Ape, Old and New World monkey species2012

    • Author(s)
      de Groot NG, et al.
    • Journal Title

      Immunogenetics

      Volume: 64(8) Issue: 8 Pages: 615-631

    • DOI

      10.1007/s00251-012-0617-1

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Analysis of immune cells in the fetal/maternal interface of the primate placenta2014

    • Author(s)
      Kametani Yoshie, Numao Erina, Mori Shuya, Kinami Rihito, Kitaura Kazutaka, Shiina Takashi, Suzuki Ryuji, Sasaki Erika
    • Organizer
      43rd Annual meeting of the Japanese Society for Immunology
    • Place of Presentation
      Kyoto, Kyoto International Conference Center
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Improved Hematopoietic Differentiation of Primate Embryonic Stem Cells2014

    • Author(s)
      Takenobu Nii, Tomotoshi Marumoto, Saori Yamaguchi, Hirotaka Kawano, Yoshie Kametani, , and Kenzaburo Tani
    • Organizer
      56th AHS Annual meeting
    • Place of Presentation
      San Francisco, CA
    • Year and Date
      2014-12-06 – 2014-12-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] コモンマーモセット胎盤における妊娠免疫系の解析2014

    • Author(s)
      森修弥、沼尾絵里奈、大島志乃、嶋田新、岡原純子、佐々木えりか、鈴木隆二、石本人士、椎名隆、亀谷美恵
    • Organizer
      第61回日本実験動物学会総会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-05-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] コモンマーモセットMHC遺伝子に置けるDNAタイピング法の開発と多型解析2014

    • Author(s)
      椎名隆、重成敦子、森修弥、北浦一孝、亀谷美恵、鈴木隆二
    • Organizer
      第61回日本実験動物学会総会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-05-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] コモンマーモセットMHC遺伝子におけるDNAタイピング法の開発と多型解析2014

    • Author(s)
      椎名隆 他
    • Organizer
      第61回日本実験動物学会総会
    • Place of Presentation
      札幌コンベンションセンター
    • Related Report
      2013 Annual Research Report
  • [Presentation] 免疫不全マウスを用いた非ヒト霊長類コモンマーモセットの造血幹細胞同定2013

    • Author(s)
      亀谷美恵
    • Organizer
      第46回日本無菌生物ノートバイオロジー学会
    • Place of Presentation
      伊勢原・フォーラム246
    • Year and Date
      2013-01-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] コモンマーモセットにおけるリアルタイムPCRを用いた免疫関連遺伝子発現解析2013

    • Author(s)
      北浦一孝 他
    • Organizer
      第60回日本実験動物学会総会
    • Place of Presentation
      茨城・筑波国際会議場
    • Related Report
      2013 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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