| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
Comparison between static and 3D conditions allowed us to conclude that physical irritant action including shear stress to contribute to differentiation to a cell growth as well as specific cell genealogy (lineage decision, for example, from a hematopoietic system cell [HSC] to megakaryocyte) and last maturity phase (i.e., platelet production from megakaryocyte). This implicated the importance of culture circumstances towards clinical application, in particular using from pluripotent stem cells. On the other hand, there is the problem of the wide “heterogeneity” of the cells during cell culture. We simultaneously clarified the importance of single cell analyzing technology useful for lineage commitment and controllable differentiation. Development of analysis technology by the single cell levels was associated with development of this research task.
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