Development of 3D cultivation system that enables to control "epigenetic change" of the cells
| Project/Area Number |
24300160
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyoto University |
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Principal Investigator |
ETO Koji 京都大学, iPS細胞研究所, 教授 (50286986)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | エピゲノム / ３D培養系 / ずり応用 / iPS細胞 / 造血幹細胞 / 巨核球 / 赤芽球 / 血小板 / 多能性幹細胞 / 細胞培養 / 細胞環境 / 3D環境 |
|---|
| Outline of Final Research Achievements |
Comparison between static and 3D conditions allowed us to conclude that physical irritant action including shear stress to contribute to differentiation to a cell growth as well as specific cell genealogy (lineage decision, for example, from a hematopoietic system cell [HSC] to megakaryocyte) and last maturity phase (i.e., platelet production from megakaryocyte). This implicated the importance of culture circumstances towards clinical application, in particular using from pluripotent stem cells. On the other hand, there is the problem of the wide “heterogeneity” of the cells during cell culture. We simultaneously clarified the importance of single cell analyzing technology useful for lineage commitment and controllable differentiation. Development of analysis technology by the single cell levels was associated with development of this research task.
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Report
(4 results)
Research Products
(80 results)
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[Journal Article] <b>Characterization of mesenchymal progenitor cells in the crown and root pulp of primary </b><b>teeth </b>2015
Author(s)
Taku TORIUMI, Naoya TAKAYAMA, Miyako MURAKAMI, Momoko SATO, Maki YUGUCHI, Yosuke YAMAZAKI, Koji ETO, Makoto OTSU, Hiromitsu NAKAUCHI, Tetsuo SHIRAKAWA, Keitaro ISOKAWA, Masaki J. HONDA.
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Journal Title
Biomedical Research
Volume: 36
Issue: 1
Pages: 31-45
DOI
NAID
ISSN
0388-6107, 1880-313X
Related Report
Peer Reviewed / Open Access
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[Journal Article] ENPP2 contributes to adipose tissue expansion and insulin resistance in diet-induced obesity.2014
Author(s)
Nishimura S, Nagasaki M, Okudaira S, Aoki J, Ohmori T, Ohkawa R, Nakamura K, Igarashi K, Yamashita H, Eto K, Uno K, Hayashi N, Kadowaki T, Komuro I, Yatomi Y, Nagai R.
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Journal Title
Diabetes
Volume: 63
Issue: 12
Pages: 4154-4164
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Generation of induced pluripotent stem cells derived from primary and secondary myelofibrosis patient samples2014
Author(s)
Hosoi M, Kumano K, Taoka K, Arai S, Kataoka K, Ueda K, Kamikubo Y, Takayama N, Otsu M, Eto K, Nakauchi H, Kurokawa M
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Journal Title
Exp Hematol
Volume: (in press)
Issue: 9
Pages: 816-25
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The generation of induced pluripotent stem cells (iPSCs) from patients with infantile and late-onset types of Pompe disease and the effects of treatment with acid-alpha-glucosidase in Pompe's iPSCs2014
Author(s)
Higuchi T, Kawagoe S, Otsu M, Shimada Y, Kobayashi H, Hirayama R, Eto K, Ida H, Ohashi T, Nakauchi H, Eto Y
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Journal Title
Molecular genetics and metabolism
Volume: 112
Issue: 1
Pages: 44-8
DOI
Related Report
Peer Reviewed
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[Journal Article] Expandable Megakaryocyte Cell Lines Enable Clinically Applicable Generation of Platelets from Human Induced Pluripotent Stem Cells.2014
Author(s)
Nakamura S, Takayama N, Hirata S, Seo H, Endo H, Ochi K, Fujita KI, Koike T, Harimoto KI, Dohda T, Watanabe A, Okita K, Takahashi N, Sawaguchi A, Yamanaka S, Nakauchi H, Nishimura S, Eto K.
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Journal Title
Cell Stem Cell.
Volume: 14
Issue: 4
Pages: 535-48
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Immortalization of Erythroblasts by c-MYC and BCL-XL Enables Large-Scale Erythrocyte Production from Human Pluripotent Stem Cells.2013
Author(s)
Hirose, S., N. Takayama, S. Nakamura, K. Nagasawa, K. Ochi, S. Hirata, S. Yamazaki, T. Yamaguchi, M. Otsu, S. Sano, N. Takahashi, A. Sawaguchi, M. Ito, T. Kato, H. Nakauchi, and K. Eto.
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Journal Title
Stem Cell Reports
Volume: 1
Issue: 6
Pages: 499-508
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] CAMT-iPS Cells Exhibiting Defective MPL Signaling Dysregulate Megakaryopoiesis and Erythropoiesis.2013
Author(s)
Shinji Hirata, Naoya Takayama,Ryoko Jono-Ohnishi, Hiroshi Endo, Sou Nakamura, Takeaki Dohda, Masanori Nishi, Yuhei Hamazaki, Ei-ichi Ishii, Shin Kaneko, Makoto Otsu, Hiromitsu Nakauchi, Shinji Kunishima, and Koji Eto.
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Journal Title
J Clin Invest.
Volume: 123
Issue: 9
Pages: 3802-14
DOI
Related Report
Peer Reviewed
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[Journal Article] Role of SOX17 in hematopoietic development from human embryonic stem cells.2013
Author(s)
Nakajima-Takagi Y, Osawa M, Oshima M, Takagi H, Miyagi S, Endoh M, Endo TA, Takayama N, Eto K, Toyoda T, Koseki H, Nakauchi H, Iwama A.
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Journal Title
Blood.
Volume: 121
Issue: 3
Pages: 447-58
DOI
Related Report
Peer Reviewed
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[Presentation] Generation and analysis of induced pluripotent stem derived from secondary myelofibrosis cells2012
Author(s)
Masataka Hosoi, Keiki Kumano, Kazuki Taoka, Shunya Arai, Koki Ueda, Yasuhiko Kamikubo, Naoya Takayama, Makoto Otsu, Koji Eto, Hiromitsu Nakauchi, Mineo Kurokawa.
Organizer
第74回日本血液学会学術集会
Place of Presentation
国立京都国際会館
Related Report
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