Cell Nanoengineering: Control of Cell Functions by External Stimuli-Responsive Nanomaterials Delivered to Specific Organelle
Project/Area Number |
24300162
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Biomedical engineering/Biological material science
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Research Institution | Kyoto University |
Principal Investigator |
Murakami Tatsuya 京都大学, 物質-細胞統合システム拠点, 特定拠点准教授 (90410737)
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Co-Investigator(Kenkyū-buntansha) |
梅山 有和 京都大学, 大学院工学研究科 分子工学専攻, 准教授 (30378806)
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Co-Investigator(Renkei-kenkyūsha) |
田中 元雅 独立行政法人 理化学研究所, 田中研究ユニット, リーダー (40321781)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
|
Keywords | ナノバイオサイエンス / 細胞工学 / 光治療 |
Outline of Final Research Achievements |
Recent rapid progress in nanotechnology has enabled development of a wide variety of photo-responsive nanomaterials. Some of them have the ability to generate heat or reactive oxygen species under illumination, which can be utilized for medical and cell biological applications. However, under physiological conditions, nanomaterials are often colloidally unstable and cytotoxic. Thus, making their surface biocompatible is essential. In this study, the applicant aimed at exploration of photo-responsiveness of carbon nanotubes and of surface chemistry for gold nanoparticles using biomaterials. As a result, it was found that (1) a semiconducting component of carbon nanotubes is able to generate reactive oxygen species very efficiently even under near-infrared light that is the least invasive to our body and (2) genetically engineered serum protein-coated gold nanoparticles is able to warm the plasma membrane of heat-sensing neurons in a site-specific manner to safely activate them.
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Report
(5 results)
Research Products
(49 results)
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[Journal Article] Cytotoxicity of pure nanodrugs of SN-38 and podophyllotoxin dimers in human cancer HepG2, KPL-4 and MCF-7 cellsc2015
Author(s)
Y. Koseki, Y. Ikuta, T. Murakami, T. Onodera, H. Oikawa, L. Cong, H. Tada, K. Gonda, N. Ohuchi, H. Kasai
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Journal Title
Mol. Cryst. Liq. Cryst.
Volume: 622
Issue: 1
Pages: 1-5
DOI
Related Report
Peer Reviewed
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[Journal Article] Journal of Controlled Release2014
Author(s)
Hashida, Y., Tanaka, H., Zhou, S., Kawakami, S., Yamashita, F., Murakami, T., Umeyama, T., Imahori, H., Hashida, M.
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Journal Title
Photo thermal ablation of tumor cells using a single-walled carbon nanotube-peptide composite
Volume: 173
Pages: 59-66
DOI
Related Report
Peer Reviewed
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[Journal Article] Preparation of immunostiumulatory single-walled carbon nanotube/CpG DNA complexes and evaluation of their potential in cancer immunotherapy2014
Author(s)
Zhou, S., Hashida, Y., Kawakami, S., Mihara, J., Umeyama, T., Imahori, H., Murakami, T. Yamashita, F. Hashida, M.
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Journal Title
International Journal of Pharmaceutics
Volume: 471
Issue: 1-2
Pages: 214-223
DOI
Related Report
Peer Reviewed
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[Journal Article] Mesoscopic Metal Nanoparticles Doubly Functionalized with natural and engineered lipidic dispersants for therapeutics2014
Author(s)
Murakami, T., Nakatsuji, H., Morone, N., Heuser, J. E., Ishidate, F., Hashida, M., Imahori, H.
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Journal Title
ACS Nano
Volume: 8
Issue: 7
Pages: 7370-7376
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Helicity Selective Photoreaction of Single-walled Carbon Nanotubes with Organosulfur Compounds in the Presence of Oxygen2013
Author(s)
Maeda, Y.; Higo, J.; Amagai, Y.; Matsui, J.; Ohkubo, K.; Yoshigoe, Y.; Hashimoto, M.; Eguchi, K.; Yamada, M.; Hasegawa, T.; Sato, Y.; Zhou, J.; Lu, J.; Miyashita, T.; Fukuzumi, S.; Murakami, T.; Tohji, K.; Nagase, S; Akasaka, T.
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Journal Title
J. Am. Chem. Soc.
Volume: 135
Issue: 16
Pages: 6356-6362
DOI
Related Report
Peer Reviewed
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[Journal Article] Photodynamic and photothermal effecrts of semiconducting and metallic-enriched single-walled carbon nanotubes2012
Author(s)
Murakami, T., Nakatsuji, H., Inada, M., Matoba, Y., Umeyama, T., Tsujimoto, M. , Isoda, S., Hashida, M., Imahori, H.
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Journal Title
Journal of the American Chemical Society
Volume: 134
Issue: 43
Pages: 17862-17865
DOI
Related Report
Peer Reviewed
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[Journal Article] Creation of pure nanodrugs and their anticancer properties2012
Author(s)
H. Kasai, T. Murakami, Y. Ikuta, Y. Koseki, K. Baba, H. Oikawa, H. Nakanishi, M. Okada, M. Shoji, M. Ueda, H. Imahori, M. Hashida
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Journal Title
Angew. Chem. Int. Ed
Volume: 51
Issue: 41
Pages: 10315-10318
DOI
Related Report
Peer Reviewed
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[Presentation] カーボンナノチューブの光線力学効果2014
Author(s)
村上達也、稲田真実、中辻博貴、梅山有和、今堀博
Organizer
第30回日本DDS学会学術集会
Place of Presentation
慶應義塾大学薬学部共立芝キャンパス
Year and Date
2014-07-30 – 2014-07-31
Related Report
Invited
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