| Project/Area Number |
24300165
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biomedical engineering/Biological material science
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
TAKAMATSU Tetsuro 京都府立医科大学, 医学(系)研究科(研究院), 教授 (40154900)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HARADA Yoshinori 京都府立医科大学, 大学院医学研究科, 講師 (10381956)
MINAMIKAWA Takeo 京都府立医科大学, 大学院医学研究科, 助教 (10637193)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
|
|---|
| Keywords | バイオイメージング / ラマン散乱分光法 / 末梢神経 / 術中診断 / メディカルイメージング / 多変量解析 / 神経温存術 / 悪性腫瘍 / 組織イメージング |
|---|
| Outline of Final Research Achievements |
Detection of peripheral nerve tissues during surgery is required to avoid neural disturbance following surgery as an aspect of realizing better functional outcome. In this study, we developed a Raman microspectroscopic system for label-free and noninvasive detection of peripheral nerves. Firstly, we constructed a Raman microspectroscope with near-infrared excitation light for deep tissue detection. Secondly, we obtained Raman spectra of peripheral nerves including myelinated nerves and unmyelinated nerves, and their adjacent tissues such as fibrous connective tissue, skeletal muscle, tunica media of blood vessel, and adipose tissue, and extracted Raman spectral features for each tissue species. Thirdly, we developed a multivariate spectral analytical method for selective detection of peripheral nerves. Our results provided feasibility of Raman microspectroscopy for molecular analysis of peripheral nerves and for selective detection of peripheral nerves in clinical use.
|
