| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Outline of Final Research Achievements |
The purpose of this study is to develop DDS for in vivo mRNA delivery and its application for treating animal disease models. By sophisticated molecular design of the cationic polymers for mRNA-loaded carriers, the parameters such as endosomal escape capacity and the stability under physiological conditions were well regulated, leading to development of protein expression-controllable system from mRNA. By introducing the mRNA-loaded carriers into subarachnoid space, the protein expression was obtained in a sustained manner for nearly a week. By administering BDNF-encoded mRNA using the carriers for model mice showing olfactory dysfunction, enhanced neurological recovery of olfactory function was achieved along with repairing the olfactory epithelium to a nearly normal architecture.
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