Maintenance and amplification of stem cells using the well-designed biomaterials to induce frustrated differentiation
| Project/Area Number |
24300173
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyushu University |
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Principal Investigator |
KIDOAKI Satoru 九州大学, 先導物質化学研究所, 教授 (10336018)
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| Co-Investigator(Renkei-kenkyūsha) |
KUBOKI Thasaneeyaa 九州大学, 先導物質化学研究所, 助教 (20526834)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Keywords | 幹細胞 / メカノバイオロジー |
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| Outline of Final Research Achievements |
Stable supply of enough numbers of stem cells are essential demand for effective regenerative medicine. However, stem cells tend to be deteriorated and differentiated into undesired lineages during culture on the conventional cell culture dishes. To maintenance its stemness with high quality and to stably amplify them, development of optimal culture technology and culture substrate or matrix are strongly required. To meet this requirement, this study focuses on construction of novel cell culture hydrogel to induce frustrated differentiation of stem cells based on the precise design of micromechanical field and elasticity patterning. Frustrated differentiation, which we had originally found before, is the phenomenon that the lineage specification of stem cells is inhibited on the microelastically-patterned hydrogels with subcellular-sized heteroritic distribution of elasticity. In this study, application of the phenomenon for stem cell manipulation and its mechanism were investigated.
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Report
(4 results)
Research Products
(28 results)
