| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
This study aimed to identify food-derived factors that affect muscle metabolism in obesity. For this purpose, we used the C2C12 murine muscle cell line. Screening based on the promoter assay suggested that soy isoflavone daidzein was a potent candidate that regulated mitochondrial biogenesis. Treatment of C2C12 myotubes with daidzein resulted in significant increases in the expression of mitochondrial transcription factor A (Tfam) as well as cytochrome b and COX1 as its targets. We found that the nuclear respiratory factor (NRF) were necessary for the effect of daidzein on Tfam expression. In addition, daidzein regulated cytokine expression through the PPAR-α and -γ in animal adipose tissue and cultured adipocytes, thereby improving the adverse effects of adipose inflammation in obesity. We also examined the effect of ketogenic nutrient environment on muscle metabolism in mice and found that ketotic states promote transcriptional regulation of various metabolic genes in muscles.
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