Ozone-oxidized cholesterols as biomarkers for metabolic syndrome and life-style related diseases
Project/Area Number |
24300257
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Eating habits, studies on eating habits
|
Research Institution | University of Shizuoka |
Principal Investigator |
OHSHIMA Hiroshi 静岡県立大学, 食品栄養科学部, 教授 (80433209)
|
Co-Investigator(Kenkyū-buntansha) |
MIYOSHI Noriyuki 静岡県立大学, 食品栄養科学部, 助教 (70438191)
GODA Toshinao 静岡県立大学, 食品栄養科学部, 教授 (70195923)
TOMONO Susumu 静岡県立大学, 食品栄養科学部, 客員研究員 (60554011)
MIYOSHI Noriyuki 静岡県立大学, 食品栄養科学部, 客員教授 (00094342)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
|
Keywords | 健康と食生活 / オゾン酸化コレステロール / 生活習慣病 / アルデヒド |
Outline of Final Research Achievements |
We have newly identified fatty-acid ester forms of secosterol-A including 9-oxononanoate secosterol-A and their secosterol-B derivatives as ozone-oxidized cholesterol fatty acid esters. These ester forms of secosterols were detected by a highly sensitive and specific LC-ESI-MS/MS method at 10~20 pmol/LDL mg protein in human low density lipoproteins obtained from healthy subjects. We further improved our method to analyze comprehensively various reactive aldehydes and carbonyls present in biological samples. We are currently using the method to study roles of reactive aldehydes and carbonyls in the development of various life-style related diseases such as arteriosclerosis, neurodegenerative diseases and cancer and also validate these reactive aldehydes including secosterols and unknowns as biomarkers for oxidative stress related disorders.
|
Report
(4 results)
Research Products
(41 results)
-
-
-
-
-
-
[Journal Article] Development and application of a method for identification of isothiocyanate-targeted molecules in colon cancer cells2012
Author(s)
Miyoshi, N., Yonemochi, T., Tomono, S., Fukutomi, R., Nakamura, Y., Ohshima, H.
-
Journal Title
Anal. Biochem.
Volume: 429(2)
Issue: 2
Pages: 124-131
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-