Roles of ESCRT in Oncogenesis
Project/Area Number |
24300326
|
Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Carcinogenesis
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Research Institution | Miyagi Prefectural Hospital Organization Miyagi Cancer Center |
Principal Investigator |
TANAKA NOBUYUKI 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん先進治療開発研究部, 部長 (60280872)
|
Co-Investigator(Kenkyū-buntansha) |
OGAMA Naoko 地方独立法人宮城県立病院機構宮城県立がんセンター(研究所), がん先進治療開発研究部, 主任研究員 (30390892)
TAMAI Keiichi 地方独立法人宮城県立病院機構宮城県立がんセンター(研究所), がん幹細胞研究部, 上席主任研究員 (40509262)
Yamaguchi Kazunori 地方独立法人宮城県立病院機構宮城県立がんセンター(研究所), 発がん研究部, 上席主任研究員 (80373215)
|
Project Period (FY) |
2012-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
|
Keywords | ESCRT / 増殖因子受容体 / 癌 / 小胞輸送 / オートファジー / プロテオーム |
Outline of Final Research Achievements |
Vesicular transport is tightly related with oncogenesis, malignant transformation and cell death. In the present study, we analyzed roles of ESCRT in vitro and in vivo. First, we found that ESRT-0 and ESCRT-I both regulate EGFR degradation. Second, AMSH-LP, a deubuiqutination enzyme associated with ESCRT-0 and ESCRT-III, may be involved in oncogenesis, and AMSH-LP knockout mice manifested shorter life span. Third, ESCRT-0 defect did not induce tumor formation, but cells defective for ESCRT-0 showed enhanced necroptosis and apoptosis, partly through the enhanced ER stress. Together, this study showed a pertinent role of ESCRT in cellular growth and death.
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Report
(5 results)
Research Products
(23 results)
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[Journal Article] ESCRT-0 dysfunction compromises autophagic degradation of protein aggregates and facilitates ER stress-mediated neurodegeneration via apoptotic and necroptotic pathways2016
Author(s)
Oshima R, Hasegawa T, Tamai K, Sugeno N, Yoshida S, Kobayashi J, Kikuchi A, Baba T, Futatsugi A, Sato I, Satoh K, Takeda A, Aoki M and Tanaka N
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 24997-24997
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Suppressive expression of CD274 increases tumorigenesis and cancer stem cell phenotypes in cholangiocarcinoma2014
Author(s)
Keiichi Tamai, Mao Nakamura, Masamichi Mizuma, Mai Mochizuki, Misa Yokoyama, Hiroyuki Endo, Kazunori Yamaguchi, Takayuki Nakagawa, Masaaki Shiina, Michiaki Unno, Koji Muramoto, Ikuro Sato, Kennichi Satoh, Kazuo Sugamura, and Nobuyuki Tanaka
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Journal Title
Cancer Science
Volume: 105
Issue: 6
Pages: 667-674
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Synergistic cytotoxicity of afatinib and cetuximab against EGFR T790M involves Rab11-dependent EGFR recycling.2014
Author(s)
Watanuki, Z.Kosai, H., Osanai, N., Ogama, N., Mochizuki, M., Tamai, K., Yamaguchi, K., Satoh, K., Fukuhara, T., Maemondo, M., Ichinose, M., Nukiwa, T., Tanaka, N.
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Journal Title
Biochem Biophy Res Commun
Volume: 455
Issue: 3-4
Pages: 269-276
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] K63-linked ubiquitination by E3 Ubiquitin Ligase Nedd4-1 facilitates endosomal sequestration of internalized alpha-synuclein.2014
Author(s)
Sugeno N, Hasegawa T, Tanaka N, Fukuda M, Wakabayashi K, Oshima R, Konno M, Miura E, Kikuchi A, Baba T, Anan T, Nakao M, Geisler S, Aoki M, Takeda A.
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Journal Title
J Biol Chem
Volume: 289
Issue: 26
Pages: 18137-18151
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Circulating miR-483-3p and miR-21 is highly expressed in plasma of pancreatic cancer.2014
Author(s)
Abue, M., Yokoyama, M., Shibuya, R., Tamai, K., Yamaguchi, K., Sato, I., Tanaka, N., Hamada, S., Shimosegawa, T., Sugamura, K., Satoh, K.
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Journal Title
Int J Oncol
Volume: 10
Issue: 2
Pages: 1-9
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] ESCRT-0 protein hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is targeted to endosomes independently of signal-transducing adaptor molecule (STAM) and the complex formation with STAM promotes its endosomal dissociation2014
Author(s)
Kojima, K., Amano, Y., Yoshino, K., Tanaka, N., Sugamura, K., Takeshita, T.
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Journal Title
J Biol Chem
Volume: 289
Issue: 48
Pages: 33296-33310
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate in the peripheral development and function of B-cells2014
Author(s)
Nagata T, Murata K, Murata R, Sun S-L, Saito Y, Yamaga S, Tanaka N, Tamai K, Moriya K, Kasai N, Sugamura K, and Ishii N.
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 443
Issue: 2
Pages: 351-356
DOI
Related Report
Peer Reviewed
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[Journal Article] Enhanced expression of long non-coding RNA HOTAIR is associated with the development of gastric cancer.2013
Author(s)
Endo H, Shiroki T, Nakagawa T, Yokoyama M, Tamai K, Yamanami H, Fujiya T, Sato I, Yamaguchi K, Tanaka N, Iijima K, Shimosegawa T, Sugamura K, Satoh K.
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Journal Title
PlosONE
Volume: 8
Issue: 10
Pages: e77070-e77070
DOI
Related Report
Peer Reviewed
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[Journal Article] Accumulation of p62/SQSTM1 is associated with poor prognosis in patients with lung adenocarcinoma2012
Author(s)
Inoue D, Suzuki T, Mitsuishi Y, Miki Y, Suzuki S, Sugawara S, Watanabe M, Sakurada A, Endo C, Uruno A, Sasano H, Nakagawa T, Satoh K, Tanaka N, Kubo H, Motohashi H, Yamamoto M
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Journal Title
Cancer Sci
Volume: 103
Issue: 4
Pages: 760-766
DOI
Related Report
Peer Reviewed
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[Journal Article] Regulation of hepatitis C virus secretion by the Hrs-dependent exosomal pathway2012
Author(s)
Tamai K, Shiina M, Tanaka N, Nakano T, Yamamoto A, Kondo Y, Kakazu E, Inoue J, Fukushima K, Sano K, Ueno Y, Shimosegawa T, Sugamura K.
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Journal Title
Virology
Volume: 22(2)
Issue: 2
Pages: 377-85
DOI
Related Report
Peer Reviewed
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