Clinical utilization of teh cell free DNA as tumor biomarker.

• Project/Area Number: 24300337
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnosis
• Research Institution: The University of Tokyo
• Principal Investigator: EIJI SUNAMI 東京大学, 医学部附属病院, 講師 (70345205)
• Co-Investigator(Kenkyū-buntansha): TSUNO Hirokazu 東京大学, 医学部附属病院, 講師 (50282637) ; KAWAI Kazushige 東京大学, 医学部附属病院, 助教 (80571942)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: 大腸がん / バイオマーカー / 血中遊離DNA / LINE1 / 大腸癌 / 腫瘍マーカー

Outline of Final Research Achievements

Circulating Cell-free DNA (cfDNA) is known to be increased in multiple cancers reflecting necrosis and apoptosis of tumor tissue and is target for various genetic analysis. The global hypomethylation is thought to correlate with genomic instability and tumor progression. In this study, we quantified the global methylation status of cfDNA in plasma extracted from colorectal cancer (CRC) patients, and investigated its clinical usefulness as a biomarker.LINE-1 hypomethylation index (LHI) in plasma may have a potential to serve as a biomarker enabling us to detect CRC patient at early stage.

Research Products

• [Journal Article] Prediction of the preoperative chemoradiotherapy response for rectal cancer by peripheral blood lymphocyte subsets. (2015)
  • Author(s): Tada N, Kawai K, Tsuno NH, Ishihara S, Yamaguchi H, Sunami E, Kitayama J, Oba K, Watanabe T.
  • Journal Title: World J Surg Oncol Volume: 13 Issue: 1 Pages: 30-30
  • DOI: 10.1186/s12957-014-0418-0
  • Peer Reviewed
• [Journal Article] Phosphatidylserine-specific phospholipase A1 (PS-PLA1) expression in colorectal cancer correlates with tumor invasion and hematogenous metastasis. (2015)
  • Author(s): Iida Y, Sunami E, Yamashita H, Hiyoshi M, Ishihara S, Yamaguchi H, Inoue A, Makide K, Tsuno NH, Aoki J, Kitayama J, Watanabe T.
  • Journal Title: Anticancer Res Volume: 35 Pages: 1459-64
  • Peer Reviewed
• [Journal Article] A phase I dose escalation study of oxaliplatin plus oral S-1 and pelvic radiation in patients with locally advanced rectal cancer (SHOGUN trial). (2015)
  • Author(s): Ishihara S, Matsusaka S, Kondo K, Horie H, Uehara K, Oguchi M, Murofushi K, Ueno M, Mizunuma N, Shinbo T, Kato D, Okuda J, Hashiguchi Y, Nakazawa M, Sunami E, Kawai K, Yamashita H, Okada T, Ishikawa Y, Nakajima T, Watanabe T.
  • Journal Title: Radiat Oncol Volume: 10 Issue: 1 Pages: 24-24
  • DOI: 10.1186/s13014-015-0333-8
  • Peer Reviewed
• [Journal Article] Nomogram prediction of metachronous colorectal neoplasms in patients with colorectal cancer. (2015)
  • Author(s): Kawai K, Ishihara S, Yamaguchi H, Sunami E, Kitayama J, Miyata H, Watanabe T.
  • Journal Title: Ann Surg Volume: 261 Issue: 5 Pages: 926-32
  • DOI: 10.1097/sla.0000000000000881
  • Peer Reviewed
• [Journal Article] Prognostic Significance of the Lymph Node Ratio in Stage IV Colorectal Cancer Patients who have Undergone Curative Resection. (2015)
  • Author(s): Ozawa T, Ishihara S, Nishikawa T, Tanaka T, Tanaka J, Kiyomatsu T, Hata K, Kawai K, Nozawa H, Kanazawa T, Kazama S, Yamaguchi H, Sunami E, Kitayama J, Watanabe T.
  • Journal Title: Ann Surg Oncol Volume: 22 Issue: 5 Pages: 1513-9
  • DOI: 10.1245/s10434-014-4184-6
  • Peer Reviewed
• [Journal Article] Detection of carcinoembryonic antigen mRNA in peritoneal lavage by the transcription-reverse transcription concerted method indicates poor prognosis in patients with stage II and III colon cancer. (2015)
  • Author(s): Murono K, Kazama S, Yamaguchi H, Kawai K, Ishihara S, Sunami E, Kitayama J, Satoh Y, Kurihara M, Yatomi Y, Watanabe T.
  • Journal Title: Surgery Volume: 157 Issue: 2 Pages: 322-30
  • DOI: 10.1016/j.surg.2014.09.015
  • Peer Reviewed
• [Journal Article] Characteristics and prognosis of colorectal cancer associated with rheumatic disease. (2015)
  • Author(s): Kishikawa J, Kawai K, Tsuno N, Yamaguchi H, Ishihara S, Sunami E, Watanabe T.
  • Journal Title: Int Surg Volume: [Epub ahead of print
  • Peer Reviewed
• [Journal Article] Incidence and prognostic significance of positive peritoneal lavage in colorectal cancer. (2015)
  • Author(s): Nishikawa T, Sunami E, Tanaka T, Tanaka J, Kiyomatsu T, Kawai K, Hata K, Kazama S, Nozawa H, Ishihara S, Watanabe T.
  • Journal Title: Surg Today Volume: Epub ahead of print
  • Peer Reviewed
• [Journal Article] Proximal shift of colorectal cancer along with aging. (2014)
  • Author(s): Iida Y, Kawai K, Tsuno NH, Ishihara S, Yamaguchi H, Sunami E, Kitayama J, Watanabe T.
  • Journal Title: Clin Colorectal Cancer Volume: 13 Issue: 4 Pages: 213-8
  • DOI: 10.1016/j.clcc.2014.06.005
  • Peer Reviewed
• [Journal Article] Prognostic impact of primary tumor resection and lymph node dissection in stage IV colorectal cancer with unresectable metastasis: a propensity score analysis in a multicenter retrospective study. (2014)
  • Author(s): Ishihara S, Hayama T, Yamada H, Nozawa K, Matsuda K, Miyata H, Yoneyama S, Tanaka T, Tanaka J, Kiyomatsu T, Kawai K, Nozawa H, Kanazawa T, Kazama S, Yamaguchi H, Sunami E, Kitayama J, Hashiguchi Y, Sugihara K, Watanabe T.
  • Journal Title: Ann Surg Oncol Volume: 21 Issue: 9 Pages: 2949-55
  • DOI: 10.1245/s10434-014-3719-1
  • Peer Reviewed / Open Access
• [Journal Article] Nuclear Notch3 expression is associated with tumor recurrence in patients with stage II and III colorectal cancer. (2014)
  • Author(s): Ozawa T, Kazama S, Akiyoshi T, Murono K, Yoneyama S, Tanaka T, Tanaka J, Kiyomatsu T, Kawai K, Nozawa H, Kanazawa T, Yamaguchi H, Ishihara S, Sunami E, Kitayama J, Morikawa T, Fukayama M, Watanabe T.
  • Journal Title: Ann Surg Oncol Volume: 21 Issue: 8 Pages: 2650-8
  • DOI: 10.1245/s10434-014-3659-9
  • Peer Reviewed
• [Journal Article] Drug development for intraperitoneal chemotherapy against peritoneal carcinomatosis from gastrointestinal cancer. (2014)
  • Author(s): Emoto S, Sunami E, Yamaguchi H, Ishihara S, Kitayama J, Watanabe T.
  • Journal Title: Surg Today Volume: 44 Issue: 12 Pages: 2209-20
  • DOI: 10.1007/s00595-014-0848-x
  • Peer Reviewed / Open Access
• [Journal Article] Changes in the plasma levels of cytokines/chemokines for predicting the response to chemoradiation therapy in rectal cancer patients. (2014)
  • Author(s): Tada N, Tsuno NH, Kawai K, Murono K, Nirei T, Ishihara S, Sunami E, Kitayama J, Watanabe T.
  • Journal Title: Oncol Rep Volume: 31 Issue: 1 Pages: 463-71
  • DOI: 10.3892/or.2013.2857
  • Peer Reviewed / Open Access
• [Presentation] 大腸癌患者におけるLINE-1を標的とした血漿遊離DNAメチル化レベルの定量と臨床的有用性の検討 (2015)
  • Author(s): 永井雄三、須並英二
  • Organizer: 第70回日本消化器外科学会
  • Place of Presentation: 浜松
  • Year and Date: 2015-07-15 – 2015-07-17