| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
DNA single-strand break is thought to be the most frequent type of DNA damage, but its significance in biological function and its repair within human cell remain to be determined. Here we identified genes involved in the regulation of SSB repair in human cell. ONe gene encodes a protein, which interacts directly with PARP1 within human cell. When its expression was suppressed with siRNA, poly(ADP-ribiosyl)ation was decreased after treatment of cells with MMS and cellular resistance to MMS decreased. Its recombinant protein has an endonuclease against AP site as well as exonuclease to both 5' and 3' directions. These data suggest that while PARP1 is able to bind SSB, for its effective activation PARP1 requires gap produced by this protein. Further analysis is required to elucidate the importance of this protein in cellular survival and oncogenesis.
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