Verification of relationships between dysfunction of the cell stress-protection system by exposures to multiple environmental chemicals and diseases

• Project/Area Number: 24310046
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: University of Toyama
• Principal Investigator: Tabuchi Yoshiaki 富山大学, 研究推進機構 研究推進総合支援センター, 教授 (20322109)
• Co-Investigator(Kenkyū-buntansha): HOSHI Nobuhiko 神戸大学, (連合)農学研究科(研究院), 教授 (10209223) ; KONDO Takashi 富山大学, 大学院医学薬学研究部(医学), 教授 (40143937)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: 内分泌かく乱物質 / 複合影響

Outline of Final Research Achievements

Using immortalized oral epithelial cells, we have identified genes and gene networks involved in sodium fluoride-elicited cell death accompanying endoplasmic reticulum stress. In a mouse experimental model, the behavioral and reproductive effects of clothianidine, a neonicotinoid pesticide, became serious in combination with environmental stress, which may reflect our actual situation of multiple exposure. These findings obtained here will provide a basis for understanding the molecular mechanisms of combined influences by exposures to multiple environmental chemicals in mammals.

Research Products

• [Journal Article] Monohydroxylated polycyclic aromatic hydrocarbon influences spicule formation in the early development of sea urchins (Hemicentrotus pulcherrimus). (2015)
  • Author(s): Suzuki, N., Ogiso, S., Yachiguchi, K., Kawabe, K., Makino, F., Toriba, A., Kiyomoto, M., Sekiguchi, T., Tabuchi, Y., Kondo, T., Kitamura, K., Hong, C., Srivastav, A.K., Oshima, Y., Hattori, A., Hayakawa, K.
  • Journal Title: Comp Biochem Physiol C Toxicol Pharmacol. Volume: 171 Pages: 55-60
  • DOI: 10.1016/j.cbpc.2015.02.004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The combined effect of clothianidin and environmental stress on the behavioral and reproductive function in male mice (2015)
  • Author(s): Hirano T, Yanai S, Omotehara T, Hashimoto R, Umemura Y, Kubota N, Minami K, Nagahara D, Matsuo E, Aihara Y, Shinohara R, Furuyashiki T, Mantani Y, Yokoyama T, Kitagawa H, Hoshi N
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 10 Pages: 1207-1215
  • DOI: 10.1292/jvms.15-0188
  • NAID: 130005107605
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Genes and gene networks involved in sodium fluoride-elicited cell death accompanying endoplasmic reticulum stress in oral epithelial cells (2014)
  • Author(s): Tabuchi Y, Yunoki T, Hoshi N, Suzuki N, Kondo T
  • Journal Title: Int J Mol Sci Volume: 15 Issue: 5 Pages: 8959-8978
  • DOI: 10.3390/ijms15058959
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effects of inorganic mercury and methylmercury on osteoclasts and osteoblasts in the scales of the marine teleost as a model system of bone (2014)
  • Author(s): Yachiguchi K, Sekiguchi T, Nakano M, Hattori A, Yamamoto M, Kitamura K, Maeda M, Tabuchi Y, Kondo T, Kamauchi H, Nakabayashi H, Srivastav AK, Hayakawa K, Sakamoto T, Suzuki N
  • Journal Title: Zoolog Sci Volume: 31 Issue: 5 Pages: 330-337
  • DOI: 10.2108/zs130265
  • NAID: 120005463340
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development of Oral Epithelial Cell Line ROE2 with Differentiation Potential from Transgenic Rats Harboring Temperature-Sensitive Simian Virus40 Large T-Antigen Gene (2014)
  • Author(s): Tabuchi Y, Wada S, Ikegame M, Kariya A, Furusawa Y, Hoshi N, Yunoki T, Suzuki N, Takasaki I, Kondo T, Suzuki Y
  • Journal Title: Experimental Animals Volume: 63 Issue: 1 Pages: 31-44
  • DOI: 10.1538/expanim.63.31
  • NAID: 130003391601
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish (2014)
  • Author(s): Yachiguchi K, Matsumoto N, Haga Y, Suzuki M, Matsumura C, Tsurukawa M, Okuno T, Nakano T, Kawabe K, Kitamura KI, Toriba A, Hayakawa K, Chowdhury VS, Endo M, Chiba A, Sekiguchi T, Nakano M, Tabuchi Y, Kondo T, Wada S, Mishima H, Hattori A, Suzuki N
  • Journal Title: Environ Sci Pollut Res Int Volume: 印刷中
  • NAID: 120005253213
  • Peer Reviewed
• [Journal Article] TGFβs modulate permeability of the blood-epididymis barrier in an in vitro model (2013)
  • Author(s): Stammler A, Muller D, Tabuchi Y, Konrad L, Middendorff R
  • Journal Title: PLoS One Volume: 8 Issue: 11 Pages: e80611-e80611
  • DOI: 10.1371/journal.pone.0080611
  • Peer Reviewed
• [Journal Article] Fetal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin transactivates aryl hydrocarbon receptor-responsive element III in the tyrosine hydroxylase immunoreactive neurons of the mouse midbrain (2013)
  • Author(s): Tanida T, Tasaka K, Akahoshi E, Ishihara-Sugano M, Saito M, Kawata S, Danjo M, Tokumoto J, Mantani Y, Nagahara D, Tabuchi Y, Yokoyama T, Kitagawa H, Kawata M, Hoshi N
  • Journal Title: Journal of Applied Toxicology Volume: Jan 8 Issue: 2 Pages: 1099-1263
  • DOI: 10.1002/jat.2839
  • Peer Reviewed / Open Access
• [Journal Article] Common gene expression patterns responsive to mild temperature hyperthermia in normal human fibroblastic cells. (2013)
  • Author(s): Tabuchi Y, Furusawa Y, Kariya A, Wada S, Ohtsuka K, Kondo T.
  • Journal Title: Int J Hyperthermia Volume: 29 Issue: 1 Pages: 38-50
  • DOI: 10.3109/02656736.2012.753163
  • Peer Reviewed
• [Presentation] 環境ストレス下においてネオニコチノイド系農薬クロチアニジンが与える神経行動学的影響 (2015)
  • Author(s): ④平野哲史，柳井翔吾，表原拓也，高田 匡，米田直起，梅村ゆりあ，久保田直人，南 貴一，広川千英，山本 杏，万谷洋平，横山俊史，北川 浩，星 信彦
  • Organizer: 環境ホルモン学会第18回研究発表会
  • Place of Presentation: 栃木県下野市
  • Year and Date: 2015-12-10
• [Presentation] 無機水銀及びメチル水銀の骨芽・破骨細胞に対する作用：メジナのウロコを用いた解析 (2014)
  • Author(s): 谷内口孝治、関口俊男、中野真樹、服部淳彦、山元 恵、田渕圭章、近藤 隆、北村敬一郎、早川和一、鈴木信雄
  • Organizer: 平成26年度日本動物学会中部支部大会
  • Place of Presentation: 石川県鳳珠郡能登町
  • Year and Date: 2014-11-22 – 2014-11-24
• [Remarks] 生命科学先端研究ユニット
  • URL: http://www.lsrc.u-toyama.ac.jp/index.htm