Generation of a new category, "Proteinaceous Insecticide" based on mechanisms which cause specificity diversity of Bacillus thuringiensis insecticidal protein.
| Project/Area Number |
24310054
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Environmental technology/Environmental materials
|
|---|
| Research Institution | Tokyo University of Agriculture and Technology |
|---|
Principal Investigator |
SATO Ryoichi 東京農工大学, (連合)農学研究科(研究院), 教授 (30235428)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
IWABUCHI Kikuo 東京農工大学, 大学院農学研究院, 教授 (00203399)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
|
|---|
| Keywords | Bacillus thuringiensis / Cry toxin / ABCC2 / receptor / 環境技術 / 環境材料 / 農薬 |
|---|
| Outline of Final Research Achievements |
To generate “Proteinaceous Insecticide” we attempted to make a method which can improve activity of the insecticidal protein made by BT. A group of insecticidal protein was indicated to act insect host using ABC transporter C2 as a receptor. It was also suggested that susceptibility of lepidopteran insects to Cry1A toxins largely depend on ABCC2s they have on the cells. To make a method by which we can evolve the binding affinity of insecticidal protein toward ABCC2 of target insects, we established large scale production system and purification system for ABCC2.We also made a platform for the screening system for the improved insecticidal proteins.
|
Report
(4 results)
Research Products
(24 results)
