Application of Rhodococcus erythropolis PR4 for bioremediations

• Project/Area Number: 24310060
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Nihon University
• Principal Investigator: IWABUCHI Noriyuki 日本大学, 生物資源科学部, 准教授 (90328708)
• Co-Investigator(Kenkyū-buntansha): OGIHARA Jun 日本大学, 生物資源科学部, 准教授 (50256830) ; FURUKAWA Soichi 日本大学, 生物資源科学部, 准教授 (40339289)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: バイオレメデーション / 有機溶媒耐性 / GroEL2 / Rhodococcus / 石油分解 / 石油汚染 / Rhodococcus属細菌 / プロテオーム / シャペロニン / Cycloclasticus属細菌 / トランスクリプトーム

Outline of Final Research Achievements

We investigated the proteins involved in the translocation of R. erythropolis PR4. The results of our proteogenomic analysis suggested that GroEL2 was upregulated more in cells that translocated inside of the pristane phase than in those located at the aqueous-alkane interface attached to the n-dodecane surface. The expression of GroEL2 in PR4 (pK4-EL2-1) was 15.5-fold higher than that in PR4 (pK4). The growths of PR4 were enhanced by the introduction of pK4-EL2-1. These results suggested that the overexpression of groEL2 led to changes in cell localization and enhanced growth. We subsequently examined the effects of the introduction of groEL2 and subsequent overexpression of GroEL2 on the alkane tolerance of various Rhodococcus strains. The results showed that the introduction of groEL2 led to an increase in alkane tolerance in some rhodococci. As results, we constructed a highly alkane-tolerant strain capable of translocating to and surviving in the presence of n-octane.

Research Products

• [Journal Article] Mg2+-dependent control of the spatial arrangement of Rhodococcus erythropolis PR4 cells in aqueous-alkane two phase culture containing n-dodecane (2016)
  • Author(s): Takihara, H., Akakse, Y., Sunairi, M., and Iwabuchi, N.
  • Journal Title: Microbes and Environmen Volume: 未定
  • NAID: 130005158501
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Transformation of lignin-derived aromatics into non-aromatic polymeric substances with fluorescent activities (NAPSFA) by Pseudomonas sp. ITH-SA-1 (2015)
  • Author(s): N. Iwabuchi, H. Takiguchi, T. Hamaguchi, H. Takihara, M. Sunairi, H. Matsufuji
  • Journal Title: ACS Sustainable Chemistry & Engineering Volume: 3 Issue: 11 Pages: 2678-2685
  • DOI: 10.1021/acssuschemeng.5b00503
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] PP-O and PP-V, Monascus pigment homologs, production and phylogenetic analysis in Penicillium purpurogenum. (2015)
  • Author(s): Arai T., Kojima R., Motegi Y., Kato J., Kasumi T., and Ogihara J.
  • Journal Title: Fungal Biology Volume: 119 Issue: 12 Pages: 1226-1236
  • DOI: 10.1016/j.funbio.2015.08.020
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Erythritol production by Moniliella megachiliensis using non-refined glycerol waste as carbon source. (2015)
  • Author(s): Kobayashi Y., Iwata H., Mizushima D., Ogihara J., and Kasumi T.
  • Journal Title: Letters in Applied Microbiology Volume: 60 Issue: 5 Pages: 475-480
  • DOI: 10.1111/lam.12391
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] <i>Rhodococcus rhodochrous</i> ATCC12674 Becomes Alkane-Tolerant upon GroEL2 Overexpression and Survives in the <i>n</i>-Octane Phase in Two Phase Culture (2014)
  • Author(s): H. Takihara, J. Ogihara, N. Iwabuchi, M. Sunairi.
  • Journal Title: Microbes and Environments Volume: 29 Issue: 4 Pages: 431-433
  • DOI: 10.1264/jsme2.ME14114
  • NAID: 130004703878
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Enhanced Translocation and Growth of <i>Rhodococcus erythropolis</i> PR4 in the Alkane Phase of Aqueous-Alkane Two Phase Cultures Were Mediated by GroEL2 Overexpression (2014)
  • Author(s): H. Takihara, J. Ogihara, T. Yoshida, S. Okuda, M. Nakajima, N. Iwabuchi, M. Sunairi.
  • Journal Title: Microbes and Environments Volume: 29 Issue: 4 Pages: 346-352
  • DOI: 10.1264/jsme2.ME13158
  • NAID: 130004693791
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Rhodococcus属細菌の有機溶媒耐性におけるgroEL2の影響 (2015)
  • Author(s): 岩淵範之、瀧原速仁、砂入道夫
  • Organizer: 日本農芸化学会大会2015大会
  • Place of Presentation: 岡山大学(岡山県岡山市)
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] アルカン相内で生育する有機溶媒耐性菌Rhodococcus erythropolis PR4のタンパク質発現プロファイルの解析 (2014)
  • Author(s): 岩淵範之、瀧原速仁、奥田修二郎、荻原淳、砂入道夫
  • Organizer: 環境微生物系合同大会2014
  • Place of Presentation: アクトシティ浜松コングレスセンター(静岡県浜松市）
  • Year and Date: 2014-10-21 – 2014-10-24
• [Presentation] Rhodococcus属細菌の有機溶媒耐性におけるgroEL2の影響 (2014)
  • Author(s): 瀧原速仁、岩淵範之、砂入道夫
  • Organizer: 環境微生物系合同大会2014
  • Place of Presentation: アクトシティ浜松コングレスセンター(静岡県浜松市)
  • Year and Date: 2014-10-21 – 2014-10-24
• [Presentation] 海洋性芳香族炭化水素分解菌CycloclasticusのRNAおよびタンパク質レベルでの遺伝子発現の網羅的解析 (2013)
  • Author(s): 岩淵範之他
  • Organizer: 第２９回微生物生態学会大会
  • Place of Presentation: 鹿児島大学
• [Presentation] 石油汚染下の微生物群集中における海洋性多環芳香族炭化水素(PAHs)分解菌Cycloclasticusの培養制御について (2013)
  • Author(s): 山下俊輔、岩淵範之, ら
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜バイオ大学
• [Presentation] Unique behavior of oil-degrading bacteria during cultivations (2012)
  • Author(s): H. Takihara, N. Iwabuchi, et al.
  • Organizer: 第4回日韓微生物シンポジウム
  • Place of Presentation: 豊橋技術科学大学(招待講演)
• [Book] Biodegradative Bacteria (2013)
  • Author(s): N. Iwbuchi
  • Total Pages: 358
  • Publisher: Springer
• [Book] Biodegradative Bacteria (2013)
  • Author(s): N. Iwbuchi
  • Publisher: Springer(印刷中)
• [Patent(Industrial Property Rights)] 蛍光物質及びその製造方法国内 (2013)
  • Inventor(s): 岩淵, ら
  • Industrial Property Rights Holder: 日本大学
  • Industrial Property Number: 2013-019971
  • Filing Date: 2013-02-04