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New bioremediation system for hydrocarbon contaminated soil using plants and microorganisms.

Research Project

Project/Area Number 24310062
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Environmental technology/Environmental materials
Research InstitutionRitsumeikan University

Principal Investigator

KUBO Motoki  立命館大学, 生命科学部, 教授 (60249795)

Co-Investigator(Kenkyū-buntansha) MORISAKI Hisao  立命館大学, 生命科学部, 教授 (50125671)
立木 隆  立命館大学, 生命科学部, 非常勤講師 (60026573)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywordsバイオレメディエーション / 石油分解菌 / 植生回復 / 炭化水素 / バイオマス / 炭化水素分解菌 / Rhdococcus / 環境技術 / 水質汚濁・土壌汚染防止・浄化 / 植物 / 微生物
Outline of Final Research Achievements

Hydrocarbon bioremediation efficiency is improved by maintaining growth and activity of hydrocarbon degrading microorganisms. In this study, new efficient bioremediation system using organic materials instead of inorganic materials was constructed. The bacterial growth and activity were extended 1week to 4 weeks using the new system, subsequently the bioremediation efficiency was drastically improved.
On the other hand, plant growth requires complete removal of the hydrocarbon in the soil. Small amount of hydrocarbon contamination in the soil (less than 10mg/kg) influences the plant growth. In this study, third generation of bioremediation system was constructed with plants and microorganisms for degradation of small amount of hydrocarbon from the soil.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (46 results)

All 2015 2014 2013 2012 Other

All Journal Article (13 results) (of which Peer Reviewed: 10 results) Presentation (24 results) (of which Invited: 1 results) Book (5 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 1 results)

  • [Journal Article] Evaluation of soil fertility for plant growth based on bacterial biomass and material circulation in soil environment2014

    • Author(s)
      M. Kubo, S. Horii, T. Matsuno, M. Mukai, and D. Adhikari
    • Journal Title

      Recent Development in Biotechnology,

      Volume: 1 Pages: 147-160

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 沖縄県内の粘土質土壌を用いた油汚染土壌のバイオパイル工法2014

    • Author(s)
      門倉伸行、佐々木静郎、村上順也、仲村紳、金城和哉、村田均、川口博史、久保幹、向真樹、Dinesh Adhikari
    • Journal Title

      環境浄化技術

      Volume: 13 Pages: 35-42

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Analysis of nitrification in agricultural soil and improvement of nitrogen circulation with autotrophic ammonia-oxidizing bacteria.2013

    • Author(s)
      T. Matsuno, S. Horii, T. Sato, Y. Matsumiya, and M. Kubo
    • Journal Title

      Applied Biochemistry and Biotechnology

      Volume: 169 Pages: 795-809

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of Hydrocarbon Degrading Bacteria in the Soil Environment and their Contribution to Bioremediation.2013

    • Author(s)
      Y. Fukuhara, S. Horii, T. Matsuno, Y. Matsumiya, M. Mukai, and M. Kubo
    • Journal Title

      Applied Biochemistry and Biotechnology

      Volume: 170 Pages: 329-339

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 有機農業における新たな土壌診断の可能性(土壌肥沃度指標:SOFIX)2013

    • Author(s)
      久保幹
    • Journal Title

      野菜情報

      Volume: 111 Pages: 2-3

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 有機農業のための畑の診断指標SOFIX2013

    • Author(s)
      久保幹
    • Journal Title

      現代農業

      Volume: 10月号 Pages: 252-255

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 地力と国力2013

    • Author(s)
      久保幹
    • Journal Title

      AFC Forum

      Volume: 10月号 Pages: 2-2

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of peptide uptake and location of root hair-promoting peptide accumnulation in plant roots.2012

    • Author(s)
      Y. Matsumiya and M. Kubo
    • Journal Title

      J. Peptide Science

      Volume: 18 Pages: 177-182

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Further stabilization of Leul55 mutant thermolysins by mutation of an autodegradation site.2012

    • Author(s)
      Y. Matsumiya and M. Kubo
    • Journal Title

      Applied Biochemistry and Biotechnology

      Volume: 166 Pages: 735-743

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 農地窒素循環の見える化 -物質循環系を考えた土づくり-2012

    • Author(s)
      松野敏英、堀井幸江、福原優樹、 松宮芳樹、久保幹
    • Journal Title

      土づくりとエコ農業

      Volume: 6・7月号 Pages: 50-55

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 油汚染土壌のバイオ処理使用微生物の安全性評価-「微生物によるバイオレメディエーション利用指針」に基づく大臣確認取得-2012

    • Author(s)
      門倉伸行、佐々木静郎、土路生修三、村上順也、久保幹、松宮芳樹
    • Journal Title

      熊谷組技術研究報告

      Volume: 70 Pages: 65-71

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 多孔性炭素を用いた油汚染土壌対策技術の開発 バイオレメディエーション技術2012

    • Author(s)
      川越大樹、吉田和生、河目裕介、東智明、久保田謙三、久保幹
    • Journal Title

      環境浄化技術

      Volume: 9・10月号 Pages: 60-63

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Soybean as a nitrogen supplier.2012

    • Author(s)
      Y. Matsumiya, S. Horii, T. Matsuno, and M. Kubo
    • Journal Title

      INTECH

      Pages: 49-60

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 嫌気・好気環境下での石油汚染土壌のバイオレメディエーションにおける菌叢解析2015

    • Author(s)
      酒井晴奈, 向 真樹, アディカリディネッシュ, 荒木希和子, 久保 幹
    • Organizer
      日本農芸化学会 2015年度大会
    • Place of Presentation
      岡山大学(岡山県)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 石油分解菌Gordonia属および Rhodococcus属におけるalk遺伝子系統解析2015

    • Author(s)
      堀口 悠, 向 真樹, Dinesh Adhikari, 荒木希和子, 久保 幹
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学(岡山県)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] アルカンヒドロキシラーゼ保持菌株の分布と役割2014

    • Author(s)
      松浦太一、Dinesh Adhikari、向真樹、荒木希和子、久保幹
    • Organizer
      第66回 日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 土壌環境中の炭化水素汚染が植物に及ぼす影響の解2014

    • Author(s)
      樋口空生、堀伸行、Dinesh Adhikari、荒木希和子、向真樹、久保幹,
    • Organizer
      第66回 日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] バイオレメディエーションに及ぼす植生の影響解析2014

    • Author(s)
      堀伸行、Dinesh Adhikari、荒木希和子、向真樹、久保幹
    • Organizer
      第66回 日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Application of useful microorganisums isolated from foods in Asian region to food production.2012

    • Author(s)
      J. T. Okumura, N. Bootim, S. Yano, Y. Matsumiya, N. Rattanakit-chandet
    • Organizer
      Asian Core Program on Capacity Building and Development of Microbial Potential and Fermentation Technology towards New Era (2008^2013)
    • Place of Presentation
      海峡メッセ下関、山口県
    • Year and Date
      2012-11-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] Utilization of Low Quality Biomass for Preserving Environment-D atabase of bacterial biomass and nitrogen circulation in soil for improvement of soil fertility-2012

    • Author(s)
      T. Matsuno, S. Horii, Y. Matsumiya, S. Sanpa, S. Yano, W. Pathom-aree, N. Rattanakit-chandet, S. Bovonsombu, A. Plikomol, T. Tachiki, M. Wakayama, and M. Kubo
    • Organizer
      Asian Core Program on Capacity Building and Development of Microbial Potential and Fermentation Technology towards New Era (2008^2013)
    • Place of Presentation
      海峡メッセ下関、山口県
    • Year and Date
      2012-11-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] Diversity and abundance of free-living nitrogen fixing bacteria isolated from various soil.2012

    • Author(s)
      S. Sanpa, S. Tasurin, Y. Matsumiya, M. Kubo, S. Yano, M. Wakayama, T. Tachiki, N. Chandet, W. Pathom-aree, and A. Plikomol
    • Organizer
      Asian Core Program on Capacity Building and Development of Microbial Potential and Fermentation Technology towards New Era (2008^2013)
    • Place of Presentation
      海峡メッセ下関、山口県
    • Year and Date
      2012-11-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] Polylactide Degradation by Actinomycetes.2012

    • Author(s)
      W. Pathom-aree, M. Konkit, A. Jarerat, N. Chandet, S. Sanpa, S. Yano, Y. Matsumiya, M. Wakayama, M. Kubo, T. Tachiki, and A. Plikomol
    • Organizer
      Asian Core Program on Capacity Building and Development of Microbial Potential and Fermentation Technology towards New Era (2008^2013)
    • Place of Presentation
      海峡メッセ下関、山口県
    • Year and Date
      2012-11-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] 共同報告「農地土壌における独立栄養性アンモニア酸化細菌と硝化の関係解析」2012

    • Author(s)
      松野敏英、堀井幸江、福原優樹、松宮芳樹、久保幹
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場、兵庫県
    • Year and Date
      2012-10-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] 共同報告「Analysis of nitrogen circulation in agricultural soil based on Soil Fertile Index」2012

    • Author(s)
      K. Ngamsomsuke, S. Horii, T. Matsuno, Y. Fukuhara, Y. Matsumiya, M. Kubo
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場、兵庫県
    • Year and Date
      2012-10-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] 共同報告「土壌肥沃度診断に基づく農地土壌のリン循環の解析」2012

    • Author(s)
      堀井幸江、松野敏英、福原優樹、松宮芳樹、久保幹
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場、兵庫県
    • Year and Date
      2012-10-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] 共同報告「バイオレメディエーションにおける炭化水素分解菌Rhodococcus erythropolis NDKK6及びGordonia terrae NDKY76Aの土壌細菌に及ぼす環境影響評価」2012

    • Author(s)
      松宮芳樹、門倉伸行、佐々木静郎、福原優樹、堀井幸江、松野敏英、久保幹
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場、兵庫県
    • Year and Date
      2012-10-25
    • Related Report
      2012 Annual Research Report
  • [Presentation] 共同報告「Rhodococcus属細菌における炭化水素分解能とアルカンヒドロキシラーゼ(alkB)遺伝子の関係解析」2012

    • Author(s)
      藤原幹大、松野敏英、福原優樹、堀井幸江、松宮芳樹、久保幹
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場、兵庫県
    • Year and Date
      2012-10-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] 共同報告「土壌環境中におけるalkB遺伝子を有する石油分解菌の分布解析」2012

    • Author(s)
      福原優樹、松野敏英、堀井幸江、松宮芳樹、久保幹
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場、兵庫県
    • Year and Date
      2012-10-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] A New Approach for an Organic Agriculture Based on Material Circulation by Environmental Microorganism2012

    • Author(s)
      M. Kubo, Y. Matsumiya, S. Sanpa, T. Matsuno, S. Horii, S. Yano, N. Rattanakit-Chandet, W. Pathom-Aree, .S. Bovonsombu, A. Plikomol., T. Tachiki, M. Wakayama
    • Organizer
      3rd MEXT-ARDA Project Concluding Joint Seminar and JSPS-NRCT Asian Core Program Joint Seminar
    • Place of Presentation
      Bangkok、Thailand
    • Year and Date
      2012-08-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] 熱溶菌性糸状菌による木質バイオマスからグルコースの生産の解析

    • Author(s)
      佐々木裕起、Dinesh Adhikari、向真樹、久保幹
    • Organizer
      第65回 日本生物工学会2013年度大会
    • Place of Presentation
      広島国際会議場(広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] アスファルテン分解糸状菌の分離及び分解能の評価

    • Author(s)
      大槻祐人、Dinesh Adhikari、向真樹、久保幹
    • Organizer
      第65回 日本生物工学会2013年度大会
    • Place of Presentation
      広島国際会議場(広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 石油分解菌Rhodococcus erythropolis NDKK6のバイオレメディエーション中における挙動解析

    • Author(s)
      久保幹、堀口悠、大槻祐人、Dinesh Adhikari、向真樹
    • Organizer
      第65回 日本生物工学会2013年度大会
    • Place of Presentation
      広島国際会議場(広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Existence of nifH, amoA and bpB genes carrying bacteria in agricultural soils.

    • Author(s)
      Chalee Kongwichian, Toshihide Matsuno, Dinesh Adhikari, Masaki Mukai, Motoki Kubo
    • Organizer
      第65回 日本生物工学会2013年度大会
    • Place of Presentation
      広島国際会議場(広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Contruction of artificial organic soil based on SOFIX(soil fertile index)

    • Author(s)
      Kanyou Ngamsomsuke, Sachie Horii, Masaki Mukai, Dinesh Adhikari , Motoki Kubo
    • Organizer
      第65回 日本生物工学会2013年度大会
    • Place of Presentation
      広島国際会議場(広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Isolation and identification of phytate degrading bacteria and their contribution to phytate mineralization in soil

    • Author(s)
      Dinesh Adhikari , Sachie Horii, Masaki Mukai, Motoki Kubo
    • Organizer
      第65回 日本生物工学会2013年度大会
    • Place of Presentation
      広島国際会議場(広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 農地土壌における土壌肥沃度(全炭素量、全窒素量、C/N比)の解析と、硝化活性の促進

    • Author(s)
      向真樹、松野敏英、Dinesh Adhikari 、久保幹
    • Organizer
      第65回 日本生物工学会2013年度大会
    • Place of Presentation
      広島国際会議場(広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] What is the Excellent Soil for Plant Growth? -Improvement of Soil Environment for Efficient Agriculture-.

    • Author(s)
      Motoki Kubo
    • Organizer
      as a keynote speaker in International Interdisciplinary Studies Seminar (IISS VI)
    • Place of Presentation
      University of Brawijaya, Malang, Indonesia
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] A COMPREHENSIVE SURVEY OF INTERNATIONAL SOYBEAN RESEARCH-GENETICS, PHYSIOLOGY, AGRONOMY AND NITROGEN RELATIONSHIPS2013

    • Author(s)
      Y. Matsumiya, S. Horii, T. Matsuno, and M. Kubo
    • Total Pages
      613
    • Publisher
      In Teck, Edited by James E. Board
    • Related Report
      2013 Annual Research Report
  • [Book] 環境微生物学2012

    • Author(s)
      久保幹、森崎久雄、久保田謙三、今中忠行
    • Publisher
      化学同人
    • Related Report
      2012 Annual Research Report
  • [Book] 生命科学1 生物個体から分子へ2012

    • Author(s)
      生命科学編集委員会編(分担執筆)
    • Publisher
      コロナ社
    • Related Report
      2012 Annual Research Report
  • [Book] 環境微生物の分析・評価 極限環境生物の産業展開2012

    • Author(s)
      久保幹(分担執筆)
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Annual Research Report
  • [Book] ひらく、ひらく「バイオの世界」2012

    • Author(s)
      日本生物工学会編(分担執筆)
    • Publisher
      化学同人
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 水浄化処理装置及び水浄化処理方法2014

    • Inventor(s)
      久保幹、向真樹、Dinesh Adhikai
    • Industrial Property Rights Holder
      久保幹、向真樹、Dinesh Adhikai
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-10-21
    • Related Report
      2014 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 植物の生育を促進する新規軽量人工土壌2013

    • Inventor(s)
      久保幹、堀井幸江、足立弘充、野中純一、西川邦之
    • Industrial Property Rights Holder
      学校法人立命館、(株)ソフト99コーポレーション、アイオン(株)
    • Industrial Property Number
      2013-013039
    • Filing Date
      2013-01-28
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 石油汚染土壌の浄化方法2012

    • Inventor(s)
      久保幹、福原優樹、河目裕介
    • Industrial Property Rights Holder
      学校法人立命館
    • Industrial Property Number
      2012-208717
    • Filing Date
      2012-09-21
    • Acquisition Date
      2012-09-21
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 環境中からの新規石油分解菌検出システム2012

    • Inventor(s)
      久保幹、福原優樹、河目裕介
    • Industrial Property Rights Holder
      学校法人立命館
    • Industrial Property Number
      2012-208715
    • Filing Date
      2012-09-21
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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