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Evaluation of the environmental nanorisk toward microbe and plant

Research Project

Project/Area Number 24310066
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Environmental technology/Environmental materials
Research InstitutionOsaka Prefecture University

Principal Investigator

NOMURA Toshiyuki  大阪府立大学, 工学(系)研究科(研究院), 准教授 (00285305)

Co-Investigator(Kenkyū-buntansha) TOKUMOTO Hayato  大阪府立大学, 大学院工学研究科, 講師 (70405348)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywordsナノリスク / 微生物細胞 / 植物細胞
Outline of Final Research Achievements

In this research, the ecotoxicity of the engineered nanoparticles toward microbe and plant was evaluated. In the case of microbe, it was found that the surface of the negatively charged microbe was entirely covered with the positively charged PSL nanoparticles owing to the electrostatic interaction, leading to the cell death. Interestingly, the PSL nanoparticles were taken inside the living yeast equipped with the rigid cell wall in the physiological saline. In the case of plant, the growth of lettuce root, lily pollen tube, and tobacco callus was inhibited by the nanoparticles and their dissolved metal ions.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (42 results)

All 2015 2014 2013 2012

All Journal Article (7 results) (of which Open Access: 1 results,  Acknowledgement Compliant: 2 results,  Peer Reviewed: 5 results) Presentation (35 results) (of which Invited: 3 results)

  • [Journal Article] Cytotoxicity of Functionalized Polystyrene Latex Nanoparticles Toward Lactic Acid Bacteria, and Comparison with Model Microbes2015

    • Author(s)
      T. Nomura, Y. Kuriyama, H. Tokumoto, Y. Konishi
    • Journal Title

      Journal of Nanoparticle Research

      Volume: 17 Issue: 2

    • DOI

      10.1007/s11051-015-2922-8

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Adhesion and internalization of functionalized polystyrene latex nanoparticles toward the yeast Saccharomyces cerevisiae2014

    • Author(s)
      J. Miyazaki, Y. Kuriyama, A. Miyamoto, H. Tokumoto, Y. Konishi, T. Nomura
    • Journal Title

      Advanced Powder Technology

      Volume: 25 Issue: 4 Pages: 1394-1397

    • DOI

      10.1016/j.apt.2014.06.014

    • NAID

      10031185746

    • Related Report
      2014 Annual Research Report
    • Open Access / Acknowledgement Compliant
  • [Journal Article] Exposure of the yeast Saccharomyces cerevisiae to functionalized polystyrene latex nanoparticles: influence of surface charge on toxicity2013

    • Author(s)
      T. Nomura, J. Miyazaki, A. Miyamoto, Y. Kuriyama, H. Tokumoto, Y. Konishi
    • Journal Title

      Environ. Sci. Technol.

      Volume: 47 Issue: 7 Pages: 3417-3423

    • DOI

      10.1021/es400053x

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ポリスチレンラテックスナノ粒子の酵母細胞への付着・取込現象の評価2013

    • Author(s)
      宮崎準平, 栗山雄太, 宮本顕久, 徳本勇人, 小西康裕, 野村俊之
    • Journal Title

      粉体工学会誌

      Volume: 50 Pages: 472-477

    • NAID

      10031185746

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PSLナノ粒子が微生物に及ぼす毒性評価2013

    • Author(s)
      野村俊之
    • Journal Title

      C&I Commun.

      Volume: 38-2 Pages: 22-24

    • NAID

      40020187342

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Bacterial Toxicity of Functionalized Polystyrene Latex Nanoparticles toward Escherichia coli2013

    • Author(s)
      J. Miyazaki, Y. Kuriyama, A. Miyamoto,
    • Journal Title

      Advanced Materials Research

      Volume: VOL. 699 Pages: 672-677

    • DOI

      10.4028/www.scientific.net/amr.699.672

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PSLナノ粒子が大腸菌に及ぼす環境毒性評価2012

    • Author(s)
      宮本顕久, 徳本勇人, 小西康裕, 野村俊之
    • Journal Title

      粉体工学会誌

      Volume: 49巻 Pages: 362-366

    • NAID

      10030764840

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] PSLナノ粒子がバイオフィルムに及ぼす毒性影響2015

    • Author(s)
      藤澤瑛梨,小西康裕,野村俊之
    • Organizer
      第17回化学工学会学生発表会(徳島大会)
    • Place of Presentation
      徳島大学(徳島)
    • Year and Date
      2015-03-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 糸状菌を用いたPSLナノ粒子の付着・取込現象の評価2015

    • Author(s)
      山本 亮,小西康裕,野村俊之
    • Organizer
      第17回化学工学会学生発表会(徳島大会)
    • Place of Presentation
      徳島大学(徳島)
    • Year and Date
      2015-03-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ポリスチレンラテックスナノ粒子の酵母細胞への付着・取込現象の評価2014

    • Author(s)
      宮崎準平,栗山雄太,宮本顕久,徳本勇人,小西康裕,野村俊之
    • Organizer
      2014年度粉体工学会秋期研究発表会
    • Place of Presentation
      東京ビッグサイト(東京都江東区)
    • Year and Date
      2014-11-25 – 2014-11-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ポリスチレンナノ粒子がバイオフィルムに及ぼす毒性影響2014

    • Author(s)
      藤澤瑛梨,小西康裕,野村俊之
    • Organizer
      2014年度粉体工学会秋期研究発表会
    • Place of Presentation
      東京ビッグサイト(東京都江東区)
    • Year and Date
      2014-11-25 – 2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 生きた微生物に働く相互作用力の直接AFM測定2014

    • Author(s)
      豊田峻介,小西康裕,野村俊之
    • Organizer
      2014年度粉体工学会秋期研究発表会
    • Place of Presentation
      東京ビッグサイト(東京都江東区)
    • Year and Date
      2014-11-25 – 2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] コロイドプローブAFM法を用いた生きた大腸菌の付着力測定2014

    • Author(s)
      豊田峻介,小西康裕,野村俊之
    • Organizer
      第46回化学工学会秋季大会
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 糸状菌への高分子ナノ粒子の取込現象の探索2014

    • Author(s)
      中川拓実,谷修治,小西康裕,野村俊之
    • Organizer
      第46回化学工学会秋季大会
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] モデル流路内での微生物細胞の付着と脱離抗力の解析2014

    • Author(s)
      吉原章仙,小西康裕,野村俊之
    • Organizer
      第46回化学工学会秋季大会
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] ナノ粒子の微生物細胞への付着・取込現象の解明とその利用技術の開発2014

    • Author(s)
      野村俊之
    • Organizer
      粉体工学会『ナノ粒子と細胞の相互作用に関するワークショップ』
    • Place of Presentation
      福岡大学(福岡)
    • Year and Date
      2014-09-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 原子間力顕微鏡を用いた生きた細胞の付着力測定2014

    • Author(s)
      豊田峻介,小西康裕,野村俊之
    • Organizer
      第16回化学工学会学生発表会(堺大会)
    • Place of Presentation
      大阪府立大学(堺市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] バイオフィルムを用いたナノ粒子の環境毒性評価2014

    • Author(s)
      伊藤式部,小西康裕,野村俊之
    • Organizer
      第16回化学工学会学生発表会(堺大会)
    • Place of Presentation
      大阪府立大学(堺市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 糸状菌を用いたポリスチレンナノ粒子の環境毒性評価2014

    • Author(s)
      中川拓実,谷修治,小西康裕,野村俊之
    • Organizer
      第16回化学工学会学生発表会(堺大会)
    • Place of Presentation
      大阪府立大学(堺市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物の茎や根の成長に酸化亜鉛ナノ粒子が与える影響の解析2014

    • Author(s)
      中島淑乃,村田森応,井渕加奈,宮崎準平,栗山雄太,竹田恵美,野村俊之,徳本勇人
    • Organizer
      第16回化学工学会学生発表会(堺大会)
    • Place of Presentation
      大阪府立大学(堺市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 酸化亜鉛ナノ粒子が嫌気微生物生態系に及ぼす変化2014

    • Author(s)
      野本健太,渡邊一史,佐藤成朗,野村俊之,徳本勇人
    • Organizer
      第16回化学工学会学生発表会(堺大会)
    • Place of Presentation
      大阪府立大学(堺市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ナノ粒子の微生物細胞への付着・取込現象の解明2014

    • Author(s)
      野村俊之
    • Organizer
      2013年度LSC研究成果発表会
    • Place of Presentation
      神戸学院大学(神戸市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 正帯電PSLナノ粒子が種々の微生物に及ぼす毒性評価2013

    • Author(s)
      栗山雄太,宮崎準平,宮本顕久,小西康裕,野村俊之
    • Organizer
      2013年度粉体工学会春期研究発表会
    • Place of Presentation
      連合会館(東京都千代田区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物体に対するPSL蛍光ナノ粒子の付着特性の解析2013

    • Author(s)
      村田森応,井渕加奈,宮崎準平,栗山雄太,野村俊之,徳本勇人
    • Organizer
      環境バイオテクノロジー学会2013年度大会
    • Place of Presentation
      北九州国際会議場(北九州市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Potential Impact of Functionalized Polystyrene Latex Nanoparticles toward Saccharomyces cerevisiae2013

    • Author(s)
      Y. Kuriyama, J. Miyazaki, H. Tokumoto, Y. Konishi, T. Nomura
    • Organizer
      7th International Conference on Materials for Advanced Technologies (ICMAT2013)
    • Place of Presentation
      Suntec (Singapore)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ecotoxicity of Functionalized Polystyrene Latex Nanoparticles Comparison between Gram Positive and Negative Bacteria2013

    • Author(s)
      Y. Kuriyama, J. Miyazaki, H. Tokumoto, Y. Konishi, T. Nomura
    • Organizer
      7th International Conference on Materials for Advanced Technologies (ICMAT2013)
    • Place of Presentation
      Suntec (Singapore)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Toxicity Test for Nanoparticles Using Biogas Produced by Enrichment Cultures of Anaerobic Sludge as an Indicator of Ecosystem Change2013

    • Author(s)
      H. Tokumoto, N. Satoh, K. Watanabe, T. Nomura
    • Organizer
      7th International Conference on Materials for Advanced Technologies (ICMAT2013)
    • Place of Presentation
      Suntec (Singapore)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Toxicity Test for ZnO Nanoparticles Using Lettuce as an Indicator of Phytotoxicity2013

    • Author(s)
      H. Tokumoto, K. Ibuchi, M. Murata, K. Kurahashi, N. Satoh, T. Nomura
    • Organizer
      7th International Conference on Materials for Advanced Technologies (ICMAT2013)
    • Place of Presentation
      Suntec (Singapore)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 菌類を用いたナノ粒子の環境リスク評価2013

    • Author(s)
      野村俊之,栗山雄太,宮崎準平,中川拓実,小西康裕,谷 修治
    • Organizer
      第51回粉体に関する討論会
    • Place of Presentation
      松江テルサ大会議室(松江市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 酸化亜鉛ナノ粒子が微生物生態系に及ぼす変化2013

    • Author(s)
      渡邊一史,佐藤成朗,栗山雄太,野村俊之,徳本勇人
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ポリスチレンナノ粒子が糸状菌に及ぼす毒性影響2013

    • Author(s)
      中川拓実,小西康裕,野村俊之,谷 修治
    • Organizer
      2013年度粉体工学会秋期研究発表会
    • Place of Presentation
      大阪南港ATC(大阪市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 酵母細胞を用いたナノ粒子の毒性発現要因の検討2013

    • Author(s)
      豊田峻介,栗山雄太,宮崎準平,小西康裕,野村俊之
    • Organizer
      2013年度粉体工学会秋期研究発表会
    • Place of Presentation
      大阪南港ATC(大阪市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 酸化亜鉛ナノ粒子が嫌気性微生物生態系に及ぼす影響2013

    • Author(s)
      渡邊一史,佐藤成朗,栗山雄太,野村俊之,徳本勇人
    • Organizer
      2013年度粉体工学会秋期研究発表会
    • Place of Presentation
      大阪南港ATC(大阪市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] タマネギをモデル植物としたナノ粒子の細胞付着特性の評価2013

    • Author(s)
      村田森応,古田雅一,野村俊之,徳本勇人
    • Organizer
      2013年度粉体工学会秋期研究発表会
    • Place of Presentation
      大阪南港ATC(大阪市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Influence of Polystyrene Latex Nanoparticles on Growth of Budding Yeast2013

    • Author(s)
      Y. Kuriyama, J. Miyazaki, H. Tokumoto, Y. Konishi, T. Nomura
    • Organizer
      The 5th Taiwan-Korea-Japan International Symposium on Microbial Ecology
    • Place of Presentation
      Jhongli(Taiwan)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Toxicity of functionalized polystyrene latex nanoparticles toward microorganisms2013

    • Author(s)
      Y. Kuriyama, H. Tokumoto, Y. Konishi, T. Nomura
    • Organizer
      The First OPU-TKU International Symposium on Frontier Chemistry and Materials for the 21st Century
    • Place of Presentation
      Osaka Prefecture University(Sakai)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ZnO nanoparticles change the ecosystem of anaerobic digestion2013

    • Author(s)
      K. Watanabe, N. Sato, Y. Kuriyama, T. Nomura, H. Tokumoto
    • Organizer
      The First OPU-TKU International Symposium on Frontier Chemistry and Materials for the 21st Century
    • Place of Presentation
      Osaka Prefecture University(Sakai)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ポリスチレンナノ粒子が出芽酵母の増殖に及ぼす影響2013

    • Author(s)
      栗山雄太,宮崎準平,徳本勇人,小西康裕,野村俊之
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ナノ粒子のリスクとその対策2012

    • Author(s)
      野村俊之
    • Organizer
      粉体技術セミナー
    • Place of Presentation
      ホテルセントノーム京都(京都)(招待講演)
    • Year and Date
      2012-12-14
    • Related Report
      2012 Annual Research Report
  • [Presentation] PSLナノ粒子の酵母細胞への付着・取込現象の評価2012

    • Author(s)
      宮崎準平, 宮本顕久, 小西康裕, 野村俊之
    • Organizer
      2012年度粉体工学会秋期研究発表会
    • Place of Presentation
      東京ビッグサイト(東京)
    • Year and Date
      2012-11-27
    • Related Report
      2012 Annual Research Report
  • [Presentation] PSLナノ粒子の微生物細胞への付着・取込2012

    • Author(s)
      宮崎準平, 宮本顕久, 小西康裕, 野村俊之
    • Organizer
      化学工学会第44回秋季大会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2012-09-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] ナノ粒子が植物体に与える毒性の評価・解析2012

    • Author(s)
      井渕加奈, 村田森応, 徳本勇人
    • Organizer
      環境バイオテクノロジー学会2012年度大会
    • Place of Presentation
      京都大学(宇治)
    • Year and Date
      2012-06-25
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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