Development of analysis method for dynamic state in high order structure of protein utilizing neutron scattering
| Project/Area Number |
24310068
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Quantum beam science
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KATO Koichi 名古屋市立大学, 薬学研究科, 教授 (20211849)
FUJII Noriko 京都大学, 原子炉実験所, 教授 (90199290)
INOUE Rintaro 京都大学, 原子炉実験所, 准教授 (80563840)
SATO Nobuhiro 京都大学, 原子炉実験所, 助教 (10303918)
OBA Yojiro 京都大学, 原子炉実験所, 助教 (60566793)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Jun-ichi 一般財団法人総合科学研究機構, 東海事業センター, 研究員 (40354899)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
|
|---|
| Keywords | 中性子小角散乱 / コントラスト変調 / 重水素化 / SANS / 部分重水素化 / 逆転コントラスト変調法 / コントラストマッチング / 複合タンパク質 |
|---|
| Outline of Final Research Achievements |
New methodology for small-angle neutron scattering corresponding to solvent contrast variation and deuteration labeling techniques has been developed and then structural investigation of protein state in an aqueous solution was performed. At first, the simulation codes were made to calculate a scattering function from PDB data considering about two techniques and a code was also made to analyze dynamical state of protein with domains with Mote Carlo algorithm. It was confirmed to be available to analyze the static and dynamical states by using them. with real protein systems: βB2-Crystatallin and proteasome activator 28. In addition, it was succeeded to prepare for 75% deuteration protein which can be matched out in 100% D2O solution.
|
Report
(4 results)
Research Products
(12 results)
-
-
-
-
[Presentation] 中性子小角散乱の基礎と応用2015
Author(s)
杉山正明
Organizer
日本化学会 第95春季年会
Place of Presentation
日本大学 理工学部船橋キャンパス（千葉県船橋市）
Year and Date
2015-03-26 – 2015-03-29
Related Report
Invited
-
-
-
-
-
-
-
-
