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Development of analysis method for dynamic state in high order structure of protein utilizing neutron scattering

Research Project

Project/Area Number 24310068
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Quantum beam science
Research InstitutionKyoto University

Principal Investigator

SUGIYAMA Masaaki  京都大学, 原子炉実験所, 教授 (10253395)

Co-Investigator(Kenkyū-buntansha) KATO Koichi  名古屋市立大学, 薬学研究科, 教授 (20211849)
FUJII Noriko  京都大学, 原子炉実験所, 教授 (90199290)
INOUE Rintaro  京都大学, 原子炉実験所, 准教授 (80563840)
SATO Nobuhiro  京都大学, 原子炉実験所, 助教 (10303918)
OBA Yojiro  京都大学, 原子炉実験所, 助教 (60566793)
Co-Investigator(Renkei-kenkyūsha) SUZUKI Jun-ichi  一般財団法人総合科学研究機構, 東海事業センター, 研究員 (40354899)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Keywords中性子小角散乱 / コントラスト変調 / 重水素化 / SANS / 部分重水素化 / 逆転コントラスト変調法 / コントラストマッチング / 複合タンパク質
Outline of Final Research Achievements

New methodology for small-angle neutron scattering corresponding to solvent contrast variation and deuteration labeling techniques has been developed and then structural investigation of protein state in an aqueous solution was performed. At first, the simulation codes were made to calculate a scattering function from PDB data considering about two techniques and a code was also made to analyze dynamical state of protein with domains with Mote Carlo algorithm. It was confirmed to be available to analyze the static and dynamical states by using them. with real protein systems: βB2-Crystatallin and proteasome activator 28. In addition, it was succeeded to prepare for 75% deuteration protein which can be matched out in 100% D2O solution.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (12 results)

All 2015 2014 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (10 results) (of which Invited: 5 results)

  • [Journal Article] Conformational characterization of a protein complex involving intrinsically disordered protein by small-angle neutron scattering using the inverse contrast matching method: a case study of interaction between α-synuclein and PbaB tetramer as a model chaperone2014

    • Author(s)
      Masaaki Sugiyama
    • Journal Title

      Journal Applied Crystallography

      Volume: 47 Issue: 1 Pages: 430-435

    • DOI

      10.1107/s1600576713033475

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatial arrangement and functional role of α subunits of proteasome activator PA28 in heteroolisomeric2013

    • Author(s)
      M. Sugiyaraa, K. Kato, et al.,
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 432 Pages: 141-145

    • Related Report
      2012 Annual Research Report
  • [Presentation] Biological SANS for next stage2015

    • Author(s)
      杉山正明
    • Organizer
      第3回Neutrons in Biology Japan研究会
    • Place of Presentation
      日本原子力研究所(茨城県那珂郡東海村)
    • Year and Date
      2015-03-31
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 中性子小角散乱の基礎と応用2015

    • Author(s)
      杉山正明
    • Organizer
      日本化学会 第95春季年会
    • Place of Presentation
      日本大学 理工学部船橋キャンパス(千葉県船橋市)
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 中性子の中性子による中性子のための生物溶液散乱2015

    • Author(s)
      杉山正明
    • Organizer
      第6回MLFシンポジウム
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2015-03-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] コントラスト変調及び重水素化で見るタンパク質の溶液中の静的・動的構造2014

    • Author(s)
      杉山正明
    • Organizer
      第37回溶液化学シンポジウム
    • Place of Presentation
      佐賀県立男女共同参画センター・佐賀県立生涯学習センター(佐賀市)
    • Year and Date
      2014-11-12 – 2014-11-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] SANS investigation on Subunit Exchange in Protein Complexes2014

    • Author(s)
      Masaaki Sugiyama
    • Organizer
      Neutrons in Bilogy and Biotechnology
    • Place of Presentation
      Grunoble, France
    • Related Report
      2013 Annual Research Report
  • [Presentation] SANS investigation of protein structure in solution2013

    • Author(s)
      杉山正明
    • Organizer
      台日中性子・X線散乱研究会
    • Place of Presentation
      KEK Tokai Campus (茨城県)(招待講演)
    • Year and Date
      2013-02-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] Application of smal1-angle NEUTRON scattering to structural study of protein complex - perspective on combination of deuteration and contrast variation method2013

    • Author(s)
      杉山正明
    • Organizer
      第2回国際シンポジウム「天然変性タンパク質の分子認識機構と機能発現」
    • Place of Presentation
      理化学研究所横浜研究所交流棟ホール(横浜市)(招待講演)
    • Year and Date
      2013-01-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] Small-angle neutron scattering for investigation of subunit kinetics of protein complex in solution2013

    • Author(s)
      Masaaki Sugiyama
    • Organizer
      International Symposium on Diffraction Structural Biology 2013
    • Place of Presentation
      Nagoya
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Kinetics in Protein Complexes Revealed by Deuteration-Assisted Small-Angle Neutron Scattering2013

    • Author(s)
      Masaaki Sugiyama
    • Organizer
      International Conference on Neutron Scattering 2013
    • Place of Presentation
      エディンバラ
    • Related Report
      2013 Annual Research Report
  • [Presentation] Structural Invesitigation of B-Crystallin in an Aqueous Solution by Small Angle Neutron Scattering2012

    • Author(s)
      Y. Ueki, M. Sugiyama
    • Organizer
      15th International Small Angle Scattering Conference
    • Place of Presentation
      Sydney Convention and ExhibitionCentre (Australia)
    • Year and Date
      2012-11-20
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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