| Budget Amount *help |
¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2013: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Outline of Final Research Achievements |
We revived classical television imaging tube technologies by use of modern nanotechnologies and developed a scanning electron optical microscope that enables us super-resolved optical observation of unstained specimen in the near-field of the imaging surface with a resolution of 62 nm. We considered the construction of an iconoscope, in which the accumulated charges due to the photoelectron emission are read out, and a flying spot scanner, in which a scanned fluorescent spot illuminates the specimen. Although the former is still under development, the latter was successfully completed in the form of a unit attachable to the specimen chamber of commercialized scanning electron microscopes. Luminescent environmental cells that enables the super-resolved observation of living biological cells were developed. The image formation principle of unstained phase objects was determined to be the Purcell effect. Finally unstained living human lung epithelial cells in liquid was observed.
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