Construction and application of single molecule observation system based on DNA nanotechnology
| Project/Area Number |
24310097
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Kyoto University |
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Principal Investigator |
ENDO Masayuki 京都大学, 物質-細胞統合システム拠点, 准教授 (70335389)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥20,930,000 (Direct Cost: ¥16,100,000、Indirect Cost: ¥4,830,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
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| Keywords | DNAナノテクノロジー / ＤＮＡオリガミ / 1分子観察 / 高速原子間力顕微鏡 / ナノ構造体 / ナノ空間 / ＤＮＡ構造変化 / 酵素反応 / DNAナノ構造体 / １分子イメージング / 光化学 / 反応制御 / エピジェネティクス / DNA組み換え反応 / 光化学反応 / DNAオリガミ / 原子間力顕微鏡 / 1分子イメージング |
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| Outline of Final Research Achievements |
In this study, we intend to elucidate dynamic biological reactions in detail using DNA nanostructures, and visualize the behavior of single biomolecule on a DNA nanostructure using high-speed atomic force microscopy (AFM). We also operate of the interaction of biomolecules and chemical reactions at the single-molecule level. We created the DNA naospace for reaction and movement of target molecules, and developed a method to directly observe the behavior of DNA structural change such as the B-Z transition and the enzyme reaction such as transcription and DNA recombination. Furthermore, we successfully created two-dimensional and three-dimensional DNA nanostructures that have photoresponsive functions, and created a system to visualize their behaviors using high-speed AFM.
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Report
(4 results)
Research Products
(64 results)
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[Journal Article] Photoresponsive DNA Nanocapsule Having an Open/Close System for Capture and Release of Nanomaterials2014
Author(s)
Takenaka, T.; Endo, M.; Suzuki, Y.; Yang, Y.; Emura, T.; Hidaka, K.; Kato, T.; Mitata, T.; Namba, K.; Sugiyama, H
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Journal Title
Chemistry - A European Journal
Volume: 20
Issue: 46
Pages: 14951-14954
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Design and development of nanosized DNA assemblies in polypod-like structures as efficient vehicles for immunostimulatory CpG motifs to immune cells2012
Author(s)
Mohri K, Nishikawa M, Takahashi N, Shiomi T, Matsuoka N, Ogawa K, Endo M, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y
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Journal Title
ACS Nano
Volume: 6
Issue: 7
Pages: 5931-5940
DOI
Related Report
Peer Reviewed
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