| Budget Amount *help |
¥20,930,000 (Direct Cost: ¥16,100,000、Indirect Cost: ¥4,830,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
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| Outline of Final Research Achievements |
In this study, we intend to elucidate dynamic biological reactions in detail using DNA nanostructures, and visualize the behavior of single biomolecule on a DNA nanostructure using high-speed atomic force microscopy (AFM). We also operate of the interaction of biomolecules and chemical reactions at the single-molecule level. We created the DNA naospace for reaction and movement of target molecules, and developed a method to directly observe the behavior of DNA structural change such as the B-Z transition and the enzyme reaction such as transcription and DNA recombination. Furthermore, we successfully created two-dimensional and three-dimensional DNA nanostructures that have photoresponsive functions, and created a system to visualize their behaviors using high-speed AFM.
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