Creation of molecular recognition tool against biological toxins and development of effective decontamination techniques

• Project/Area Number: 24310125
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Social systems engineering/Safety system
• Research Institution: National Research Institute of Police Science
• Principal Investigator: Seto Yasuo 科学警察研究所, 法科学第三部, 部長 (10154668)
• Co-Investigator(Kenkyū-buntansha): UZAWA Hirotaka 国立研究開発法人産業技術総合研究所, ナノシステム研究部門, 上級主任研究員 (60356566) ; OHSAWA Isaku 科学警察研究所, 法科学第三部, 主任研究官 (30370886) ; MIYAGUCHI Hajime 科学警察研究所, 附属鑑定所, 鑑定官 (10370884) ; 柘 浩一郎 科学警察研究所, 法科学第三部, 主任研究官 (90356204)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
• Keywords: テロ対策 / 分子認識 / 機器分析 / 生物毒素 / 吸着

Outline of Final Research Achievements

As evaluation method for molecular recognition tool against biological toxins, surface plasmon resonance (SPR) analytical method and electrospray ionization spectrometry have been developed. As for preparation of candidate molecular recognition tool, artificial antibody against Staphylococcal enterotoxin B (SEB) has been created using phage display method, and also lactose derivatives which reduction terminal are combined with triethylene glycol azide has been synthesized. The most suitable candidate of the commercial antibody preparation against SEB has been selected by measurement of binding affinity and capacity using SPR. Lactose derivatives have been immobilized into monolithic silica by click chemistry-mediated sugar introduction method. The prepared sugar-immobilized monolithic silica tip has been verified for decontamination power capturing ricin.

Research Products

• [Journal Article] バイオテロ対策としての微生物現場制御 (2015)
  • Author(s): 瀬戸康雄
  • Journal Title: 日本防菌黴学会誌 Volume: ４３ Pages: 191-200
  • Peer Reviewed
• [Journal Article] バイオテロ対処としての微生物現場制御 (2015)
  • Author(s): 瀬戸康雄
  • Journal Title: 日本防菌防黴学会誌 Volume: 43 Pages: 191-200
  • Peer Reviewed / Open Access
• [Journal Article] Glycotechnology for decontamination of biological agents : A model study using ricin and biotin-tagged synthetic glycopolymers (2012)
  • Author(s): T. Nagatsuka, H. Uzawa, K. Sato, I. Ohsawa。Y. Seto and Y. Nishida
  • Journal Title: ACS Applied Materials & Interfaces Volume: 4 Issue: 2 Pages: 832-837
  • DOI: 10.1021/am201493q
  • Peer Reviewed
• [Presentation] DEVELOPMENT OF ON-SITE DECONTAMINATION TECHNOLOGIES – PHOTOCATALYTIC DEGRADATION FOR CHEMICAL WARFARE AGENTS AND MOLECULAR RECOGNITION ADSORPTION FOR BIOLOGICAL TOXINS (2013)
  • Author(s): Yasuo Seto, Takeshi Ohmori, Mieko Kanamori-Kataoka, Kouichiro Tsuge, Isaac Ohsawa, Shintaro Kishi, Yuji Urushibata, Keita Sato, Asuka Komano, Hirotaka Uzawa, Takehiro Nagatsuka, Koji Takeuchi, Nobuaki Negishi, Tsutomu Hirakawa and Masahiro Furuno
  • Organizer: 11th International symposium on Protection against Chemical and Biological Warfare agents
  • Place of Presentation: Stockholm