| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Outline of Final Research Achievements |
As evaluation method for molecular recognition tool against biological toxins, surface plasmon resonance (SPR) analytical method and electrospray ionization spectrometry have been developed. As for preparation of candidate molecular recognition tool, artificial antibody against Staphylococcal enterotoxin B (SEB) has been created using phage display method, and also lactose derivatives which reduction terminal are combined with triethylene glycol azide has been synthesized. The most suitable candidate of the commercial antibody preparation against SEB has been selected by measurement of binding affinity and capacity using SPR. Lactose derivatives have been immobilized into monolithic silica by click chemistry-mediated sugar introduction method. The prepared sugar-immobilized monolithic silica tip has been verified for decontamination power capturing ricin.
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