| Budget Amount *help |
¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
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| Outline of Final Research Achievements |
The application of the in vitro fertilization (IVF) in assisted reproductive technology has been increased since its first introduction. Recently, the intracytoplasmic sperm injection (ICSI) technique is used in about half of the IVF cases in Japan.
The aim of this study is to evaluate the effects of ICSI on the transcription regulation in human early development and to improve the ICSI method. First, we successfully established the RNA amplification protocol for single embryo RNA-seq analysis using T7 RNA polymerase. Then we analyzed the gene expression profile of the human blastocysts that were not transferred in assisted reproductive technologies. As the results, we identified the genes that affected their expression by ICSI procedure. These genes expected to be the useful markers to improve the ICSI technique.
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