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The effects of the intracytoplasmic sperm injection on the gene expression regulation in the human preimplantation embryo.

Research Project

Project/Area Number 24310141
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Genome biology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KOHDA Takashi  東京医科歯科大学, 難治疾患研究所, 准教授 (60211893)

Co-Investigator(Renkei-kenkyūsha) KUBOTA Toshiro  東京医科歯科大学, 大学院医歯学総合研究科, 教授 (50126223)
TSUTSUMI Osamu  国際医療福祉大学, 大学院医療福祉学研究科, 教授 (60134574)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Keywords顕微受精 / 体外受精 / 遺伝子発現 / エピゲノム / 受精 / 顕微授精
Outline of Final Research Achievements

The application of the in vitro fertilization (IVF) in assisted reproductive technology has been increased since its first introduction. Recently, the intracytoplasmic sperm injection (ICSI) technique is used in about half of the IVF cases in Japan.
The aim of this study is to evaluate the effects of ICSI on the transcription regulation in human early development and to improve the ICSI method. First, we successfully established the RNA amplification protocol for single embryo RNA-seq analysis using T7 RNA polymerase. Then we analyzed the gene expression profile of the human blastocysts that were not transferred in assisted reproductive technologies. As the results, we identified the genes that affected their expression by ICSI procedure. These genes expected to be the useful markers to improve the ICSI technique.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (8 results)

All 2014 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results)

  • [Journal Article] Effects of embryonic manipulation and epigenetics.2013

    • Author(s)
      kohda T
    • Journal Title

      J Hum Genet

      Volume: 58 Issue: 7 Pages: 416-420

    • DOI

      10.1038/jhg.2013.61

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Embryo manipulation via assisted reproductive technology and epigenetic asymmetry in mammalian early development.2012

    • Author(s)
      Kohda T.
    • Journal Title

      Phillos Trans R Soc Lond B Biol Sci

      Volume: 368 Issue: 1609 Pages: 20120353-20120353

    • DOI

      10.1098/rstb.2012.0353

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Zygotic gene activationと顕微授精によって誘導される遺伝子発現変化2014

    • Author(s)
      幸田 尚、高木 清考、及川 真実、越後貫 成美、井上 貴美子、金児-石野 知子、小倉 淳郎、石野 史敏
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Gene expression changes induced by intracitoplasmic sperminjection.2013

    • Author(s)
      Takashi Kohda, Kiyotaka Takagi, Mami Oikawa, Narumi Ogonuki, Kimiko Inoue, Tomoko Kaneko-Ishino,TeruhikoWakayama, Atsuo Ogura,Fumitoshi Ishino
    • Organizer
      11^<th> Transgenic Technology Meeting
    • Place of Presentation
      広州白雲国際会議センター(中国)(招待講演)
    • Year and Date
      2013-02-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] 顕微授精によって発生初期に誘導される遺伝子発現の変化2013

    • Author(s)
      幸田尚、高木清考、及川真実、越後貫成美、井上貴美子、小倉淳郎、金児-石野知子、石野史敏
    • Organizer
      第7回日本エピジェネティクス研究会年会
    • Place of Presentation
      奈良県新公会堂(奈良県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] マウス初期胚で顕微授精によって誘導される遺伝子発現調節の変化2013

    • Author(s)
      幸田尚、高木清考、及川真実、越後貫成美、井上貴美子、金児石野知子、小倉淳郎、石野史敏
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 顕微授精によって最初に誘導される遺伝子発現調節の変化2012

    • Author(s)
      幸田尚、高木清考、及川真実、越後貫成美、井上貴美子、金児・石野子、小倉淳郎、石野史敏
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2012-12-11
    • Related Report
      2012 Annual Research Report
  • [Presentation] 顛微授精によって誘導される遺伝子発現の変化2012

    • Author(s)
      幸田尚、高木清考、井上貴美子、越後貫成美、若山照彦、金児-石野子、小倉淳郎、石野史敏
    • Organizer
      第6回日本エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター(東京都)
    • Year and Date
      2012-05-15
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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