New site-specific recombination systems for genome engineering, VCre/VloxP and SCre/SloxP
| Project/Area Number |
24310143
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Kazusa DNA Research Institute |
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Principal Investigator |
NAKAYAMA MANABU 公益財団法人かずさDNA研究所, 技術開発研究部, チーム長 (30370927)
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| Co-Investigator(Renkei-kenkyūsha) |
KOSEKI Haruhiko 理化学研究所・統合生命医科学研究センター, 免疫器官形成研究グループ, グループディレクター (40225446)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2012: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | バイオテクノロジー / 部位特異的組み換え / 病態モデル動物 / 有用遺伝子探索 / 染色体工学 / 遺伝子改変動物 / ゲノム編集 / ゲノム操作技術 / 遺伝子 / 動物 / 機能ゲノム / 発生・分化 / 病態モデルマウス / ゲノム |
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| Outline of Final Research Achievements |
Two popular tools used in genomic engineering are the Cre/loxP and Flp/FRT site-specific recombination systems, which are often used to develop conditional knockout mice. Alternative combinations of recombination systems, in addition to the Cre/loxP and Flp/FRT systems, can be useful tools for genetically modifying genomes. We have developed two novel site-specific recombination systems named VCre/VloxP and SCre/SloxP for genome engineering. VCre and SCre recombinases originate from Vibrio sp. and Shewanella sp. ANA-3, respectively. Because their recognition sites are different from Cre recognition sites, we can use these site-specific recombination systems simultaneously. Moreover, we have identified some additional site-specific recombination systems derived from other species. These new site-specific recombination systems can serve as powerful tools for genome engineering, especially when used to genetically modify both alleles of a single gene in mouse and human cells.
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Report
(4 results)
Research Products
(4 results)
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[Journal Article] Generating a transgenic mouse line stably expressing human MHC surface antigen from a HAC carrying multiple genomic BACs.2015
Author(s)
Hasegawa Y, Ishikura T, Hasegawa T, Watanabe T, Suzuki J, Nakayama M, Okamura Y, Okazaki T, Koseki H, Ohara O, Ikeno M, and Masumoto H
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Journal Title
Chromosoma
Volume: 124(1)
Issue: 1
Pages: 107-118
DOI
Related Report
Peer Reviewed / Open Access
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