| Budget Amount *help |
¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2012: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Outline of Final Research Achievements |
Two popular tools used in genomic engineering are the Cre/loxP and Flp/FRT site-specific recombination systems, which are often used to develop conditional knockout mice. Alternative combinations of recombination systems, in addition to the Cre/loxP and Flp/FRT systems, can be useful tools for genetically modifying genomes. We have developed two novel site-specific recombination systems named VCre/VloxP and SCre/SloxP for genome engineering. VCre and SCre recombinases originate from Vibrio sp. and Shewanella sp. ANA-3, respectively. Because their recognition sites are different from Cre recognition sites, we can use these site-specific recombination systems simultaneously. Moreover, we have identified some additional site-specific recombination systems derived from other species. These new site-specific recombination systems can serve as powerful tools for genome engineering, especially when used to genetically modify both alleles of a single gene in mouse and human cells.
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