| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Outline of Final Research Achievements |
Next-generation sequencing was applied to small RNA fractions with lengths ranging from 40 to 140 nt from mouse brain and secondary structure-based clustering was performed. From the hundred million sequences obtained, 16 candidates of the mouse structured small non-coding RNAs (MsncRs) were isolated. Their expressions, together with those of other candidates, were analyzed by northern hybridization. One among 16 candidates obtained by the next-generation sequencing revealed a signal shorter than expected.
One of the candidates, MsncR-11, have a sequence complementary to a mitochondrial tRNA. NMR analysis suggested that MsncR-11 forms a secondary structure similar to tRNA including acceptor and T stems.
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