| Budget Amount *help |
¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Outline of Final Research Achievements |
We performed genome wide analysis of efficiency in mRNA translation to protein, employing new method. We experimentally showed that rate of initiation largely affected the overall efficiency of a gene, while difference in elongation efficiency by codon usage did exist but the effect was much smaller.
We also found new insights in the nature of translation apparatus and factors, such as efficiency of non-canonical codon-anticodon recognition at 3rd letter and translational regulation by sRNA/Hfq, as well as effect of nascent-peptide sequence on specificity of various antibiotics.
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