Development and applications of high-efficiency gene targeting in human cells
| Project/Area Number |
24310149
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied genomics
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| Research Institution | Yokohama City University |
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Principal Investigator |
ADACHI NORITAKA 横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 教授 (30264675)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | ゲノム改変 / ジーンターゲティング / ランダム挿入 |
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| Outline of Final Research Achievements |
We have been developing systems that enable rapid production of human cell lines with the use of plasmid-based DNA vectors. In this research project, we have conducted basic studies aimed at developing ultrahigh-efficiency gene-targeting systems, particularly in terms of controlling random integration, a mechanism that acts to reduce the efficiency of gene targeting.
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Report
(4 results)
Research Products
(9 results)
