Discovery of coumarin prenyltransferase family and the regulation of catalytic function
| Project/Area Number |
24310156
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Akifumi 京都大学, 生存圏研究所, 助教 (20598601)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | プレニル基転移酵素 / クマリン / ジメチルアリルジリン酸 / ゲラニルジリン酸 / 膜結合性酵素 / 色素体局在 / 柑橘類 / アシタバ / O-プレニル化 / 二次代謝 / 生合成 / 膜タンパク質 / プレニルジリン酸 |
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| Outline of Final Research Achievements |
Prenylated coumarins widely occur in Apiaceae and Rutaceae. They show toxicity to herbivores and pathogens serving as protectants for plants that produce those metabolites, while those coumarin compounds show a variety of biological activities to humans, as well. Prenyltransferase, a key enzyme in the biosynthesis, has been first identified at the gene level from parsley (Apiaceae) and lemon (Rutaceae).
Prenyltransferase family for coumarins show high hydrophobicity via typically nine transmembrane alpha-helices, and they show strict specificity to both prenyl donor and acceptor substrates. For the catalytic function, Mg2+ is required and the subcellular localization of these membrane proteins is demonstrated to be plastids in plant cells.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Molecular cloning and characterization of a geranyl diphosphate-specific aromatic prenyltransferase from lemon2014
Author(s)
Ryosuke Munakata, Tsuyoshi Inoue, Takao Koeduka, Fazeelat Karamat, Alexandre Olry, Akifumi Sugiyama, Kojiro Takanashi, Audray Dugrand, Yann Froelicher, Ryo Tanaka, Yoshihiro Uto, Hitoshi Hori, Jun-Ichi Azuma, Alain Hehn, Frédéric Bourgaud, and Kazufumi Yazaki
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Journal Title
Plant physiology
Volume: 166
Issue: 1
Pages: 80-90
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Metabolic engineering of flavonoids with prenyltransferase and chalcone isomerase genes in tomato fruits2014
Author(s)
Kawasaki, K., Koeduka, T., Sugiyama, A., Sasaki, K., Linley, PJ., Shitan, S., Kumano, T., Yamamoto, H., Ezura, H., Kuzuyama, T., Yazaki, K.
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Journal Title
Plant Biotechnology
Volume: 31
Issue: 5
Pages: 567-571
DOI
NAID
ISSN
1342-4580, 1347-6114
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] パースニップのフラノクマリン経路において膜結合型プレニル化酵素はリニア型 / アンギュラー型 生合成の運命を決定する2015
Author(s)
棟方涼介, Fazeelat KARAMAT, Alexandre OLRY, Courdavault VINCENT, 杉山暁史, 伊達慶明, Celia KRIEGER, Nicolas PAPON, Emilien FOURREAU, Jeremy GROSJEAN, 矢崎一史, Frederic BOURGAUD, Alain HEHN
Organizer
日本農芸化学会岡山大会
Place of Presentation
岡山市
Year and Date
2015-03-26 – 2015-03-29
Related Report
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[Presentation] Membrane-bound dimethylallyltransferase for umbelliferone catalyzes the first committed biosynthetic reaction in furanocoumarin formation2014
Author(s)
Ryosuke Munakata, Karamat Fazeelat, Alexandre Olry, Takao Koeduka, Masaharu Mizutani, Célia Krieger, Akifumi Sugiyama, Cedric Paris, Alain Hehn, Frédéric Bourgaud, Kazufumi Yazaki
Organizer
The XXVIIth International Conference on Polyphenols & The 8th Tannin Conference
Place of Presentation
Nagoya
Year and Date
2014-09-02 – 2014-09-06
Related Report
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