| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Outline of Final Research Achievements |
To detect the DNA helix bending by a chemical approach, duplexes containing 2-O-mercaptoalkyl-β-D-arabinofuranose on both sides of the major groove were synthesized, and the effects of the cisplatin adduct, the abasic site analog, and the (6-4) photoproduct on DNA structures were investigated. A disulfide bond was formed depending on the cisplatin adduct, and dynamic DNA bending was shown for the abasic site and the (6-4) photoproduct. Using the bent structure obtained by this method, the model proposed for the handover of damaged DNA from the UV-DDB protein to the XPC protein in the nucleotide excision repair was validated.
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