| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
In order to search for new anti-microbial substances against the dormant Mycobacterium tuberculosis, which shows tolerance to the conventional anti-Tuberculosis drugs, we constructed several screening systems focusing on the characteristic phenotypes of mycobacteria at the infected region. On the guidance of the screening from the extract library of marine sponges and marine-derived microbes, we could explore several anti-microbial compounds including structurally new compounds, which are expected to be a new medicinal seed. In addition, we could clarify the target molecules of some isolated anti-microbial compounds by the method utilizing the transformants overexpressing genomic DNA library, the analysis of gene mutation and/or the pull-down assay using a synthetic probe of the active compound. The clarified target proteins are expected to become a new drug target against the dormant M. tuberculosis.
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