| Project/Area Number |
24310162
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | The University of Tokushima (2013-2014)
Hokkaido University (2012) |
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Principal Investigator |
NAMBA Kosuke 徳島大学, ヘルスバイオサイエンス研究部, 教授 (50414123)
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| Co-Investigator(Kenkyū-buntansha) |
MURATA Yoshiko 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 主席研究員 (60256047)
ODA Masataka 新潟大学, 大学院医歯薬系, 准教授 (00412403)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | シデロフォア / 1,3a,6a-トリアザペンタレン / 蛍光プローブ / ビブリオフェリン / エンテロバクチン / 蛍光発色団 / トリアザペンタレン / 細菌検出 / 鉄イオン取り込み / 微生物検出 |
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| Outline of Final Research Achievements |
Novel smallest fluorescent molecules were developed, and they were introduced to siderophore that is secreted by microorganism as a natural iron chelator. We succeeded to produce various compact fluorescent molecules that cover all color region from blue to red on the basis of new fluorescent chromophore 1,3a,6a-triazapentalene originally developed in our laboratory . In addition, vibrioferrin and enterobactin as the siderophore of Vibrio parahaemolyticus and salmonella respectively were efficiently synthesized. Furthermore, the introduction of various TAPs to vivrioferrin and enterobactin was also achieved. The detection and identification of specific microorganism by using TAP-siderophore are currently underway in our laboratory.
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