| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
We have previously described a hairpin primer PCR (HP-PCR) method using a primer having a hairpin tag at the 5′ end and a fluorescent dye (2,7-diamino-1,8-naphthyridine derivative [DANP]) bound to the cytosine bulge (C-bulge) embedded in the hairpin-tag. It was turn-off type of system. In this research, we tried to develop the high-sensitivity detection method using a HP-PCR and it applied to detect some kinds of virus.
In this research, we developed the new type of PCR monitoring system using DANP bound to the primers as a covalent and in which the fluorescent signal increases as PCR progresses. This was a turn-on of PCR using fluorescently and modified DNA.
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