Novel high-sensitivity detection method of virus using a hairpin primer PCR
Project/Area Number |
24310165
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Chemical biology
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Research Institution | National Defense Medical College (2014-2015) Osaka University (2012-2013) |
Principal Investigator |
Fumie Takei 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 医学教育部医学科進学課程, 准教授 (30252711)
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Co-Investigator(Renkei-kenkyūsha) |
NAKAKTNI Kazuhiko 大阪大学, 産業科学研究所, 教授 (70237303)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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Keywords | PCR / 蛍光分子 / 遺伝子検出 / ウイルス検出 / グアニン / シトシンバルジ構造 / RT-PCR / へアピンプライマー |
Outline of Final Research Achievements |
We have previously described a hairpin primer PCR (HP-PCR) method using a primer having a hairpin tag at the 5′ end and a fluorescent dye (2,7-diamino-1,8-naphthyridine derivative [DANP]) bound to the cytosine bulge (C-bulge) embedded in the hairpin-tag. It was turn-off type of system. In this research, we tried to develop the high-sensitivity detection method using a HP-PCR and it applied to detect some kinds of virus. In this research, we developed the new type of PCR monitoring system using DANP bound to the primers as a covalent and in which the fluorescent signal increases as PCR progresses. This was a turn-on of PCR using fluorescently and modified DNA.
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Report
(5 results)
Research Products
(16 results)
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[Presentation] Toward new FET devices detecting DNA2014
Author(s)
Rajiv Kumar Verma, Akiko Michikawa, Norhayati Binti Sabani, Fumie Takei, and Kazuhiko Nakatani
Organizer
3rd imec Handai International Symposium
Place of Presentation
大阪
Year and Date
2014-12-12
Related Report
Int'l Joint Research
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