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Nano-space functional analysis of biological molecules by IR super-resolution microscopy

Research Project

Project/Area Number 24350003
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Physical chemistry
Research InstitutionTokyo Institute of Technology

Principal Investigator

SAKAI MAKOTO  東京工業大学, 資源化学研究所, 准教授 (60298172)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords超解像 / 赤外分光 / イメージング / ナノ空間 / 振動和周波
Outline of Final Research Achievements

IR microscope is a powerful tool to measure molecular images of biological samples based on molecular information. However, microscopic objects such as biological cells cannot be measured by conventional IR microscope, because of its low spatial resolution about 10 μm. To overcome this problem, we have developed a novel type of IR super-resolution microscope based on vibrational sum-frequency generation detection. The application of this IR microscope to the biological samples was also carried out in this project.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (35 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (28 results) (of which Int'l Joint Research: 4 results,  Invited: 10 results) Remarks (1 results)

  • [Journal Article] Mass Analyzed Threshold Ionization Detected Infrared Spectroscopy: Isomerization Activity of the Phenol–Ar Cluster near the Ionization Threshold2015

    • Author(s)
      Mitsuhiko Miyazaki, Shunpei Yoshikawa, François Michels, Kentaro Misawa, Shun-ichi Ishiuchi, Makoto Sakai, Otto Dopfer, Klaus Müller-Dethlefs, Masaaki Fujii
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 17 Issue: 4 Pages: 2949-2503

    • DOI

      10.1039/c4cp04584j

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 赤外超解像顕微鏡法2015

    • Author(s)
      酒井誠,藤井正明
    • Journal Title

      高分子

      Volume: 64 Pages: 588-589

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] 赤外顕微鏡によるがん細胞の生体反応計測技術2015

    • Author(s)
      酒井誠
    • Journal Title

      光学

      Volume: 38 Pages: 344-349

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] STED, 蛍光ディップ手法における高解像度実現の共通原理2014

    • Author(s)
      酒井誠,藤井正明
    • Journal Title

      O plus E

      Volume: 36 Pages: 147-151

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Quaternary and secondary structural imaging of a human hair by a VSFG-detected IR super-resolution microscope2013

    • Author(s)
      Makoto Sakai, Katsuya Kikuchi, Masaaki Fujii
    • Journal Title

      Chem. Phys.

      Volume: 419 Pages: 261-265

    • DOI

      10.1016/j.chemphys.2013.02.016

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] IR super-resolution microspectroscopy and its application to single cells2013

    • Author(s)
      Makoto Sakai, Keiichi Inoue, Masaaki Fujii
    • Journal Title

      Curr. Pharm. Bio techno.

      Volume: 14 Issue: 2 Pages: 159-166

    • DOI

      10.2174/1389201011314020005

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Orientation-sensitive IR imaging of feather β-keratins using a VSFG detected IR super-resolution microscopy2016

    • Author(s)
      Yukihisa Watase, Kohei Ushio, Masaaki Fujii, Makoto Sakai
    • Organizer
      The 3rd Tokyo Tech-Rutgers ICC Workshop in NAIST
    • Place of Presentation
      奈良先端科学技術大学院大学
    • Year and Date
      2016-01-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Vibrational sum-frequency generation detected IR super-resolution micro-spectroscopy of human hair α-keratins2016

    • Author(s)
      Makoto Sakai, Kohei Ushio, Yukihisa Watase, Masaaki Fujii
    • Organizer
      The 3rd Tokyo Tech-Rutgers ICC Workshop in NAIST
    • Place of Presentation
      奈良先端科学技術大学院大学
    • Year and Date
      2016-01-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Orientation-sensitive IR imaging of feather β-keratins by a VSFG-detected IR super-resolution microscopy2015

    • Author(s)
      Yukihisa Watase, Kohei Ushio, Masaaki Fujii, Makoto Sakai
    • Organizer
      International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem 2015)
    • Place of Presentation
      Hawaii Convention Center、Honolulu
    • Year and Date
      2015-12-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Observation of molecular orientation of human hair α-keratin fibers by a VSFG detected IR super-resolution microscopy2015

    • Author(s)
      Makoto Sakai, Kohei Ushio, Yukihisa Watase, Masaaki Fujii
    • Organizer
      International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem 2015)
    • Place of Presentation
      Hawaii Convention Center、Honolulu
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 赤外超解像顕微鏡法による羽毛β-ケラチンの分子配向イメージング2015

    • Author(s)
      渡瀬五常、牛尾公平、藤井正明、酒井誠
    • Organizer
      第9回分子科学討論会
    • Place of Presentation
      京工業大学大岡山キャンパス
    • Year and Date
      2015-09-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] VSFG検出赤外超解像顕微鏡法による毛髪α-ケラチンの分子配向イメージング -各振動モードにおける偏光依存性の測定-2015

    • Author(s)
      牛尾公平、渡瀬五常、藤井正明、酒井誠
    • Organizer
      第9回分子科学討論会
    • Place of Presentation
      東京工業大学大岡山キャンパス
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] VSFG検出赤外超解像顕微鏡法を用いた羽毛β-ケラチンの分子配向イメージング2015

    • Author(s)
      渡瀬五常、牛尾公平、藤井正明、酒井誠
    • Organizer
      第53回生物物理学会年会
    • Place of Presentation
      金沢大学角間キャンパス
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 赤外超解像顕微鏡法による毛髪α-ケラチンの分子配向イメージング -振動モード毎の偏光依存性測定-2015

    • Author(s)
      牛尾公平、渡瀬五常、藤井正明、酒井誠
    • Organizer
      第53回生物物理学会年会
    • Place of Presentation
      金沢大学角間キャンパス
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] VSFG検出赤外超解像顕微鏡法を用いた羽毛β-ケラチンの赤外分光イメージング~Amide I バンドの偏光依存性測定~2015

    • Author(s)
      渡瀬五常、牛尾公平、藤井正明、酒井誠
    • Organizer
      平成27年度日本分光学会年次講演会
    • Place of Presentation
      東京工業大学大岡山キャンパス
    • Year and Date
      2015-06-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Observation of molecular orientation of human hair α-keratins by a VSFG detected IR super-resolution microspectroscopy2015

    • Author(s)
      Makoto Sakai
    • Organizer
      Kick-off symposium on International Collaboration in Chemistry Project on excited state dynamics of molecules in close proximity to nanostructured metal surfaces
    • Place of Presentation
      New Jersey, USA
    • Year and Date
      2015-01-15 – 2015-01-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Super-resolving IR imaging of biological samples by using vibrational sum-frequency generation detection2015

    • Author(s)
      Makoto Sakai
    • Organizer
      Department lecture of Brookhaven National Laboratory
    • Place of Presentation
      New York, USA
    • Year and Date
      2015-01-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 赤外超解像イメージング法による生体分子のナノ空間機能解析2014

    • Author(s)
      酒井誠
    • Organizer
      ナノマクロ物質・デバイス・システム創製アライアンスG3分科会
    • Place of Presentation
      九州大学西新プラザ,福岡
    • Year and Date
      2014-11-21 – 2014-11-22
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 振動和周波検出赤外超解像顕微鏡法による毛髪試料の分子構造解析2014

    • Author(s)
      酒井誠、渡瀬五常、牛尾公平、石川春樹、藤井正明、長瀬忍、伊藤隆司
    • Organizer
      第52回生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター、札幌
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 赤外超解像顕微鏡法による毛髪α-ケラチンの分子配向イメージング2014

    • Author(s)
      牛尾公平、渡瀬五常、石川春樹、藤井正明、酒井誠
    • Organizer
      第8回分子科学討論会
    • Place of Presentation
      広島大学、東広島
    • Year and Date
      2014-09-21 – 2014-09-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] VSFG検出赤外超解像顕微鏡法による毛髪α-ケラチンの分子配向イメージ ング2014

    • Author(s)
      酒井誠
    • Organizer
      第6回SFG研究会シンポジウム
    • Place of Presentation
      筑波大学,筑波
    • Year and Date
      2014-08-02 – 2014-08-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 赤外超解像顕微鏡を使って生体試料を観察する2014

    • Author(s)
      酒井誠
    • Organizer
      第48 回量子物理化学セミナー兼理数GPセミナー
    • Place of Presentation
      横浜市立大学,横浜
    • Year and Date
      2014-07-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 赤外超解像顕微鏡で生体試料を観察する2014

    • Author(s)
      酒井誠
    • Organizer
      分光イノベーション研究会第3回シンポジウム
    • Place of Presentation
      理化学研究所,和光
    • Year and Date
      2014-05-28 – 2014-05-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 振動和周波検出赤外超解像顕微鏡法による毛髪α‐ケラチンの分子配向イメージング2014

    • Author(s)
      牛尾公平、長瀬忍、平野祐司、伊藤隆司、藤井正明、酒井誠
    • Organizer
      平成 26 年度日本分光学会年次講演会
    • Place of Presentation
      理化学研究所,和光
    • Year and Date
      2014-05-26 – 2014-05-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 赤外超解像顕微鏡を使って生体試料を観察する:原理から実測まで2014

    • Author(s)
      酒井誠
    • Organizer
      平成25年度日本分光学会生細胞分光部会シンポジウム
    • Place of Presentation
      松山
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 赤外超解像顕微鏡による生体試料観察2013

    • Author(s)
      酒井誠
    • Organizer
      東京工業大学キャンパス交流フォーラム・プラスワン
    • Place of Presentation
      東京工業大学, 東京
    • Year and Date
      2013-03-01
    • Related Report
      2012 Annual Research Report
  • [Presentation] Secondary and quaternary structural imaging of human hairs by using VSFG-detected IR super-resolution microscope2013

    • Author(s)
      Makoto SAKAI and Masaaki FUJII
    • Organizer
      The 16th International Conference on Time-resolved Vibrational Spectroscopy
    • Place of Presentation
      Beppu, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] Observation of human hair samples by a VSFG detected IR super-resolution microscope2013

    • Author(s)
      Makoto Sakai
    • Organizer
      The 7th International Conference on Advanced Vibrational Spectroscopy
    • Place of Presentation
      Kobe, Japan
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] VSFG 検出赤外超解像顕微鏡による毛髪α-ケラチンの分子配向観察2013

    • Author(s)
      酒井誠、牛尾公平、長瀬忍、平野祐司、伊藤隆司、石川春樹、藤井正明
    • Organizer
      第7回分子科学討論会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] 振動和周波検出赤外超解像顕微鏡による毛髪α-ケラチンの分子配向観察2013

    • Author(s)
      酒井誠、牛尾公平、長瀬忍、平野祐司、伊藤隆司、石川春樹、藤井正明
    • Organizer
      第51回日本生物物理学会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] Observation of biological samples using an infrared super-resolution microscope2013

    • Author(s)
      Makoto Sakai
    • Organizer
      The 17th East Asian Workshop on Chemical Dynamies
    • Place of Presentation
      Kyusyu Univ., Fukuoka(招待講演)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of a mid-IR super-resolution microscope and its application tobiological samples2012

    • Author(s)
      Makoto Sakai
    • Organizer
      The 50th Annual Meeting of the Biophysical Society of Japan, Symposium on "Star of life shined by frontier microscopies"
    • Place of Presentation
      Nagoya Univ., Nagoya
    • Year and Date
      2012-09-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] 振動和周波発生法を利用した赤外超解像顕微鏡の開発と生細胞への応用2012

    • Author(s)
      酒井誠、藤井正明
    • Organizer
      日本膜学会第34年会境界領域膜シンポジウム
    • Place of Presentation
      早稲田大学, 東京(招待講演)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 2波長レーザー分光法を利用した超解像赤外分光イメージングシステムの開発2012

    • Author(s)
      酒井誠
    • Organizer
      応用物理学会第49回光波センシング技術研究会
    • Place of Presentation
      東京理科大学, 東京(招待講演)
    • Related Report
      2012 Annual Research Report
  • [Remarks] 藤井・酒井研究室 東京工業大学資源化学研究所分光化学部門

    • URL

      http://www.csd.res.titech.ac.jp/indexj.html

    • Related Report
      2015 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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