| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
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| Outline of Final Research Achievements |
Several techniques for high-sensitive vibrational sum-frequency generation (SFG) spectroscopy to investigate biological molecular layers in aqueous conditions have been developed or examined. The techniques include (1) high-sensitive heterodyne-detected vibrational SFG spectrometer with a very-thin quartz crystal as the local oscillator, (2) nano-particle enhanced vibrational SFG, and (3) prism-parallel substrate coupled systems for vibrational SFG at total reflection geometries. As applications, surface-pressure dependent structures of lipid layers on water and silica substrates (Langmuir and Langmuir-Blodgett monolayers) and molecular orientations and structures of proteins and water at surface of proteins aqueous solutions have been investigated.
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